Ecology and Resilient Infrastructure

Korean Society of Ecology and Infrastructure Engineering (응용생태공학회)
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The Effect of Soil Amended with β-glucan under Drought Stress in Ipomoea batatas L.

𝛽-glucan 토양혼합에 따른 고구마의 가뭄피해 저감 효과

  • Jung-Ho Shin (Department of Integrated Food, Bioscience and Biotechnology) ;
  • Hyun-Sung Kim (Department of Bioenergy Science and Technology, Chonnam National University) ;
  • Gwan-Ju Seong (Department of Bioenergy Science and Technology, Chonnam National University) ;
  • Won Park (National Institute of Crop Science, Rural Development Administration) ;
  • Sung-Ju Ahn (Department of Bioenergy Science and Technology, Chonnam National University)
  • 신정호 (전남대학교 융합식품바이오공학과) ;
  • 김현성 (전남대학교 바이오에너지공학과) ;
  • 성관주 (전남대학교 바이오에너지공학과) ;
  • 박원 (농촌진흥청 국립식량과학원) ;
  • 안성주 (전남대학교 바이오에너지공학과)
  • Received : 2023.09.11
  • Accepted : 2023.09.24
  • Published : 2023.09.30
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Abstract

Biopolymer is a versatile material used in food processing, medicine, construction, and soil reinforcement. 𝛽-glucan is one of the biopolymers that improves the soil water content and ion adsorption in a drought or toxic metal contaminated land for plant survival. We analyzed drought stress damage reduction in sweet potatoes (Ipomoea batatas L. cv. Sodammi) by measuring the growth and major protein expression and activity under 𝛽-glucan soil amendment. The result showed that sweet potato leaf length and width were not affected by drought stress for 14 days, but sweet potatoes grown in 𝛽-glucan-amended soil showed an effect in preventing wilting caused by drought in phenotypic changes. Under drought stress, sweet potato leaves did not show any changes in electrolyte leakage, but the relative water content was higher in sweet potatoes grown in 𝛽-glucan-amended soil than in normal soil. 𝛽-glucan soil amendment increased the expression of plasma membrane (PM) H+-ATPase, but it decreased the aquaporin PIP2 (plasma membrane intrinsic protein 2) in sweet potatoes under drought stress. Moreover, water maintenance affected the PM H+-ATPase activity, which contributed to tolerance under drought stress. These results indicate that 𝛽-glucan soil amendment improves the soil water content during drought and affects the water supply in sweet potatoes. Consequently, 𝛽-glucan is a potential material for maintaining soil water contents, and analysis of the major PM proteins is one of the indicators for evaluating the biopolymer effect on plant survival under drought stress.

𝛽-glucan은 바이오폴리머 (biopolymer)의 한 종류로 식품 및 의약품 산업에 이용되고 있으며, 최근 친환경신소재로서 제방강화에 이용되거나 토양에 배합하여 식생을 보호하는 연구가 이루어지고 있다. 본 연구에서는 바이오폴리머 중 𝛽-glucan의 토양혼합 유무와 가뭄처리에 따른 괴근작물 고구마 (Ipomoea batatas L., 품종명 소담미)의 표현형, 생장, 그리고 주요 단백질의 발현 및 활성 변화를 분석하였다. 가뭄 스트레스 하에서 𝛽-glucan 토양혼합에 따른 고구마의 엽장 및 엽폭의 생장, 그리고 전해질유출도에서 큰 차이가 나타나지 않았으나. 상대수분함량은 통계적으로 유의성을 보여주었다. 가뭄스트레스 내성에 관여된 주요 원형질막 (plasma membrane, PM) 단백질의 발현과 활성을 분석하였을 때, 1차 능동수송체 PM H+-ATPase은 𝛽-glucan 토양혼합 조건과 가뭄스트레스에 하에서 상대적으로 높은 발현과 활성을 유지하였으나, 수분수송단백질 아쿠아포린 plasma membrane intrinsic protein 2 (PIP2)은 𝛽-glucan 토양혼합 조건과 가뭄스트레스에 의해 원형질막에서의 분포가 감소하였다. 이 결과는 𝛽-glucan의 토양혼합이 가뭄스트레스 하에서 토양수분 보유력을 향상시켜 고구마의 가뭄 스트레스와 관련된 원형질막 단백질들이 내성에 유리하게 발현됨을 보여준다. 결론적으로 이 연구는 바이오폴리머를 활용한 토양생태를 조절하는 기술로써 가뭄에 따른 식생의 생장 및 피해를 판단하는데 유효할 것이라 판단된다.

Keywords

Acknowledgement

본 연구는 국토교통부 물관리연구사업 (사업번호: 18AWMP-B114119-03)과 2023년 광주광역시 (광주테크노파크) 대학 혁신역량강화 기획 지원사업의 지원에 의해 이루어진 결과로 이에 감사드립니다.

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