BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Quercetin induces dual specificity phosphatase 5 via serum response factor

  • Kanokkan Boonruang (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Ilju Kim (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Chaeyoung Kwag (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Junsun Ryu (Department of Otolaryngology-Head and Neck Surgery, Center for Thyroid Cancer, Research Institute and Hospital, National Cancer Center) ;
  • Seung Joon Baek (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • 투고 : 2023.04.05
  • 심사 : 2023.06.05
  • 발행 : 2023.09.30
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초록

The phytochemical quercetin has gained attention for its anti-inflammatory and anti-tumorigenic properties in various types of cancer. Tumorigenesis involves the aberrant regulation of kinase/phosphatase, highlighting the importance of maintaining homeostasis. Dual Specificity Phosphatase (DUSP) plays a crucial role in controlling the phosphorylation of ERK. The current study aimed to clone the DUSP5 promoter, and investigate its transcriptional activity in the presence of quercetin. The results revealed that quercetin-induced DUSP5 expression is associated with the serum response factor (SRF) binding site located in the DUSP5 promoter. The deletion of this site abolished the luciferase activity induced by quercetin, indicating its vital role in quercetin-induced DUSP5 expression. SRF protein is a transcription factor that potentially contributes to quercetin-induced DUSP5 expression at the transcriptional level. Additionally, quercetin enhanced SRF binding activity without changing its expression. These findings provide evidence of how quercetin affects anti-cancer activity in colorectal tumorigenesis by inducing SRF transcription factor activity, thereby increasing DUSP5 expression at the transcriptional level. This study highlights the importance of investigating the molecular mechanisms underlying the anti-cancer properties of quercetin, and suggests its potential use in cancer therapy.

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과제정보

This work was supported by the Research Institute for Veterinary Science and BK21 PLUS Program for Creative Veterinary Science Research Center, Seoul National University, and by a National Research Foundation of Korea (NRF) grant funded by the Korean government (2021R1A2B2002923). We are particularly grateful for the technical assistance of Mr. Jaehak Lee at the Seoul National University.

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