The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of Jayangdaebo-tang before and after fermentation on antioxidant, anti-inflammatory and anticancer effects

자양대보탕(滋養大補湯)의 발효 전·후 항염증, 항암, 항산화 효과 비교 연구

  • Juyoung Kim (Jayang Hospital of Korean Medicine) ;
  • Eunseo Hong (College of Korean Medicine, Dongguk University) ;
  • Yong-Ki Park (College of Korean Medicine, Dongguk University) ;
  • Hyo Won Jung (College of Korean Medicine, Dongguk University)
  • Received : 2023.07.21
  • Accepted : 2023.09.25
  • Published : 2023.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : In this study, it was investigated the anti-inflammatory, anticancer, and antioxidant effects of Jayangdaebo-tang (JDT) consisting of twelve herbs before and after fermentation. Methods : JDT extract was fermented using the Lactoplantibacillus plantanum (JDT-L), Bacillus subtilis (JDT-B), and L. plantanum plus B. subtilis (JDT-L+B). The effects of each extract were measured in LPS-stimulated RAW264.7 cells, MCF-7 breast cancer and A549 lung cancer cells, and H2O2-stimulated HepG2 cells. Results : The extracts of JDT-L, JDT-B and JDT-L+B at 1 ㎎/㎖ decreased significantly the levels of nitric oxide (NO) in LPS-treated RAW264.7 cells and also inhibited the expression of iNOS and COX-2, and the phosphorylation of ERK and NF-κB. The JDT-L+B extract decreased significantly the expression of apoptotic proteins, Bax, cleaved caspase-3, and PARP in MCF-7 and A549 cancer cells. The JDT-L, JDT-B and JDT-L+B extracts increased significantly the cell viability in H2O2-stimulated HepG2 cells and the JDT-L+B extract decreased significantly the expression of SOD, catalase, HO-1, and NRF-2. Among fermented JDT extracts, JDT-L+B was the best effective on response of macrophage inflammation, cancer cell apoptosis, and liver cell damage. Conclusions : Our results were suggested that the fermentation can be used as a useful way to enhance the biological activity of JDT.

Keywords

References

  1. Kim SH. The study of the fermented herbal's usefulness for the improvement in efficiency of the oriental medicine. Graduate School of Alternative Medicine, Kyonggi University. 2008.
  2. Kim YM. A Study on the Trend of Researches in Fermented Herb Medicines. Graduate School of Kyungwon University. 2009.
  3. Yoon SD. A study on the drinking in Dong Ui Bo Gam. Graduate School of yunghee University. 2012.
  4. Lee H. A study on the Brewing method in Euibangyoochui. Graduate School of Kyunghee University. 2015.
  5. Jeong JW. The efficacy and safety of Sipjeondaebotang for cancer patients: A systematic review and meta-analysis. Graduate School of Kyung Hee University. 2023.
  6. Oh S, Cheon C, Park S, Jang BH, Park JS, Jang S, Shin YC, Ko SG. The Analysis of the Recent Research Trend of Sipjeondabo-tang in Korea. Journal of Society of Preventive Korean Medicine 2014;18(1): 113-123
  7. Ministry of Food and Drug Safety. URL : Http://ezdrug.mfds.go.kr
  8. Jeon WY, Shin IS, Shin HK, Lee MY. Gastroprotective effect of the traditional herbal medicine, Sipjeondaebo-tang water extract, against ethanol-induced gastric mucosal injury. BMC Complement Altern Med. 2014; 14: 373.
  9. Cheon C, Park S, Park YL, Huang CW, Ko Y, Jang BH, Shin YC, Ko SG. Sipjeondaebo-tang in patients with cancer with anorexia: a protocol for a pilot, randomised, controlled trial. BMJ Open. 2016; 6(5): e011212.
  10. Yang MC, Jeong SW, Ma JY. Analysis of Constituents in Sipjundaebo-tangs Fermented by Lactic Acid Bacteria. Korean J. Microbiol. Biotechnol. 2011; 39(4): 350-356.
  11. Oh YC, Cho WK, Jeong YH, Im GY, Yang MC, Ma JY. Fermentation improves anti-inflammatory effect of sipjeondaebotang on LPS-stimulated RAW 264.7 cells. Am J Chin Med. 2012; 40(4): 813-31.
  12. National Herbal Medicine Information. URL : https://nifds.go.kr/nhmi/hbdc/ofcmhbdc/list.do
  13. Lee JT. Research on intake of Chinese medicine by Korean. Ministry of Food and Drug Safety Report. 2006.
  14. Oh S, Cheon C, Park S, Jang BH, Park JS, Jang S, Shin YC, Ko SG. The Analysis of the Recent Research Trend of Sipjeondabo-tang in Korea. Journal of Society of Preventive Korean Medicine 2014;18(1):113-23.
  15. Textbook Compilation Committee of National Universityof Korean Medicine, Korea. Herbology 4th ed. Seoul : Younglimsa. 2020 : 217.
  16. Wang ZL, Wang S, Kuang Y, Hu ZM, Qiao X, Ye M. A comprehensive review on phytochemistry, pharmacology, and flavonoid biosynthesis of Scutellaria baicalensis. Pharm Biol. 2018 ; 56(1): 465-484. https://doi.org/10.1080/13880209.2018.1492620
  17. Textbook Compilation Committee of National Universityof Korean Medicine, Korea. Herbology 4th ed. Seoul : Younglimsa. 2020 : 345.
  18. Zhao YY. Traditional uses, phytochemistry, pharmacology, pharmacokinetics and quality control of Polyporus umbellatus (Pers.) Fries: a review. J Ethnopharmacol. 2013; 149(1): 35-48.
  19. Textbook Compilation Committee of National Universityof Korean Medicine, Korea. Herbology 4th ed. Seoul : Younglimsa. 2020 : 375.
  20. Sharma S, Shukla MK, Sharma KC, Tirath, Kumar L, Anal JMH, Upadhyay SK, Bhattacharyya S, Kumar D. Revisiting the therapeutic potential of gingerols against different pharmacological activities. Naunyn Schmiedebergs Arch Pharmacol. 2023; 396(4): 633-47. https://doi.org/10.1007/s00210-022-02372-7
  21. Textbook Compilation Committee of National Universityof Korean Medicine, Korea. Herbology 4th ed. Seoul : Younglimsa. 2020 : 645.
  22. Textbook Compilation Committee of National Universityof Korean Medicine, Korea. Herbology 4th ed. Seoul : Younglimsa. 2020 : 231.
  23. Shang ZP, Wang F, Zhang JY, Wang ZJ, Lu JQ, Wang HY, Li N. The genus Liriope: Phytochemistry and pharmacology. Chin J Nat Med. 2017; 15(11): 801-15. https://doi.org/10.1016/S1875-5364(18)30014-1
  24. Haixia Y, Meng Y, Xinmin H, Xinqiang N. The Therapeutic Effect of the Chinese Herbal Medicine, Rehmanniae Radix Preparata, in Attention Deficit Hyperactivity Disorder via Reversal of Structural Abnormalities in the Cortex. Evid Based Complement Alternat Med. 2018; 2018: 3052058.
  25. Zhu B, Qi F, Wu J, Yin G, Hua J, Zhang Q, Qin L.Red Yeast Rice: A Systematic Review of the Traditional Uses, Chemistry, Pharmacology, and Quality Control of an Important Chinese Folk Medicine. Front Pharmacol. 2019; 10: 1449.
  26. Huang L, Li HJ, Wu YC. Processing technologies, phytochemistry, bioactivities and applications of black ginseng-a novel manufactured ginseng product: A comprehensive review. Food Chem. 2023; 407: 134714.
  27. Yan JK, Wang WQ, Wu JY. Recent advances in Cordyceps sinensis polysaccharides: Mycelial fermentation, isolation, structure, and bioactivities: A review. J Funct Foods. 2014; 6: 33-47. https://doi.org/10.1016/j.jff.2013.11.024
  28. Wang N, Wu TX, Zhang Y, Xu XB, Tan S, Fu HW. Experimental analysis on the main contents of Rhizoma gastrodiae extract and inter-transformation throughout the fermentation process of Grifola frondosa. Arch Pharm Res. 2013; 36(3): 314-21. https://doi.org/10.1007/s12272-013-0029-2
  29. Kotas ME, Medzhitov R. Homeostasis, inflammation, and disease susceptibility. Cell. 2015; 160(5): 816-27. https://doi.org/10.1016/j.cell.2015.02.010
  30. Murakami A, Ohigashi H. Targeting NOX, INOS and COX-2 in inflammatory cells: chemoprevention using food phytochemicals. Int J Cancer. 2007; 121(11): 2357-63. https://doi.org/10.1002/ijc.23161
  31. Won S, Ko JH, Jeon H, Park SS, Kim SN. CoAdministration of Gagam-Sipjeondaebo-Tang and Ibuprofen Alleviates the Inflammatory Response in MPTP-Induced Parkinson's Disease Mouse Model and RAW264.7 Macrophages. Pathogens. 2021; 10(3): 268.
  32. Chae SW. Function and Activation of NF-kappa B in Immune System. Korean J Otolaryngol 2005; 48: 284-88.
  33. Park HS, Mo JS, CHoi EJ. Nitric oxide inhibits an interaction between JNK1 and c-Jun through nitrosylation. BBRC 2006; 1(8): 281-86. https://doi.org/10.1016/j.bbrc.2006.10.034
  34. Seo JH, Lim JW, Kim H. Differential Role of ERK and p38 on NF-κB Activation in Helicobacter pylori-Infected Gastric Epithelial Cells. J Cancer Prev. 2013; 18(4): 346-50. https://doi.org/10.15430/JCP.2013.18.4.346
  35. Naser R, Fakhoury I, El-Fouani A, Abi-Habib R, El-Sibai M. Role of the tumor microenvironment in cancer hallmarks and targeted therapy (Review). Int J Oncol. 2023; 62(2): 23.
  36. Hassan M, Watari H, AbuAlmaaty A, Ohba Y, Sakuragi N. Apoptosis and molecular targeting therapy in cancer. Biomed Res Int. 2014; 2014: 150845.
  37. Rezaei M, Heidari M, Peluso I, Ghorat F, Bishayee A, Kooti W. Medicinal Plants in the Prevention and Treatment of Colon Cancer. Aiello P, Sharghi M, Mansourkhani SM, Ardekan AP, Jouybari L, Daraei N, Peiro K, Mohamadian S, Oxid Med Cell Longev. 2019; 2019: 2075614.
  38. Husain S, Hillmann K, Hengst K, Englert H. Effects of a lifestyle intervention on the biomarkers of oxidative stress in non-communicable diseases: A systematic review. Front Aging. 2023; 4: 1085511.
  39. Alharbi KS, Almalki WH, Albratty M, Meraya AM, Najmi A, Vyas G, Singh SK, Dua K, Gupta G. The therapeutic role of nutraceuticals targeting the Nrf2/HO-1 signaling pathway in liver cancer. J Food Biochem. 2022; 46(10): e14357.
  40. Li B, Nasser MI, Masood M, Adlat S, Huang Y, Yang B, Luo C, Jiang N. Role of Nrf2/HO-1 system in development, oxidative stress response and diseases: an evolutionarily conserved mechanism. Biomed Pharmacother. 2020; 126: 110074.
  41. Tu M, Fan X, Shi J, Jing S, Xu X, Wang Y. 2-Fluorofucose Attenuates Hydrogen PeroxideInduced Oxidative Stress in HepG2 Cells via Nrf2/keap1 and NF-κB Signaling Pathways. Life (Basel). 2022; 12(3): 406.
  42. Xu J, Zhang Y, Ren G, Yang R, Chen J, Xiang X, Qin H, Chen J. Inhibitory Effect of Delphinidin on Oxidative Stress Induced by H2O2 in HepG2 Cells. Oxid Med Cell Longev. 2020; 2020: 4694760.
  43. Chen L, Li K, Liu Q, Quiles JL, Filosa R, Kamal MA, Wang F, Kai G, Zou X, Teng H, Xiao J. Protective effects of raspberry on the oxidative damage in HepG2 cells through Keap1/Nrf2-dependent signaling pathway. Food Chem Toxicol. 2019; 133: 110781.
  44. Park HR, Lee H, Park H, Cho WK, Ma JY Fermented Sipjeondaebo-tang Alleviates Memory Deficits and Loss of Hippocampal Neurogenesis in Scopolamine-induced Amnesia in Mice. Sci Rep. 2016; 6: 22405.