Korean Journal of Clinical Pharmacy (한국임상약학회지)

Korean College Of Clinical Pharmacy (한국임상약학회)
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Hematocrit Determination using a Volumetric Absorptive Microsampling Technique in Patients with Pancreatic Cancer

  • Yeolmae Jung (Department of Pharmacy, College of Pharmacy, Seoul National University) ;
  • Seunghyun Yoo (Department of Pharmacy, College of Pharmacy, Seoul National University) ;
  • Minseo Kang (Department of Pharmacy, College of Pharmacy, Seoul National University) ;
  • Hayun Lim (Department of Pharmacy, College of Pharmacy, Seoul National University) ;
  • Myeong Hwan Lee (Department of Internal Medicine and Liver Research Institute, Seoul National University Hospital, Seoul National University College of Medicine) ;
  • Ji Kon Ryu (Department of Internal Medicine and Liver Research Institute, Seoul National University Hospital, Seoul National University College of Medicine) ;
  • Jangik Lee (Department of Pharmacy, College of Pharmacy, Seoul National University)
  • Received : 2023.08.28
  • Accepted : 2023.09.19
  • Published : 2023.09.30
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Abstract

Background: Hematocrit is usually measured from venous blood collected by invasive venipuncture. This study was performed to determine hematocrit accurately and precisely using minimally invasive volumetric absorptive microsampling (VAMS) technique. Such technique is to be applied to determining hematocrit in various clinical settings for the care, including therapeutic drug monitoring, of neonatal or epileptic patients, or patients with high risk of infection or bleeding. Methods: The study was performed using 31 VAMS samples obtained from 21 pancreatic cancer patients. Hematocrit was determined using the values of potassium concentrations obtained from blood in VAMS tips (HctVAMS). HctVAMS was compared with hematocrit measured from blood collected by venipuncture (HctVP). The accuracy and precision of HctVAMS in comparison to HctVP were evaluated using Bland-Altman plot, Deming regression and mountain plot. Results: Bland-Altman plot displayed a random scattering pattern of the differences between HctVAMS and HctVP with the mean bias of -0.010 and the 95% limit of agreement ranging from -0.063 to 0.044. Deming regression for HctVAMS and HctVP line demonstrated very small proportional and constant biases of 1.04 and -0.003, respectively. Mountain plot exhibited a narrow and symmetrical distribution of the differences with their median of -0.011 and central 95% range from -0.049 to 0.033. Conclusion: Hematocrit was accurately and precisely determined using less invasive VAMS technique. Such technique appears to be applicable to determining hematocrit in situations that venipuncture is not favorable or possible.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2021R1F1A104996113). This work was also supported by the four phases of the Brain Korea 21 Program in 2023. The authors appreciate the patients who participated in this study. The authors also thank Hyeongseok Lee who conducted the bioanalytical part of this study.

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