한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제36권4호
  • /
  • Pages.244-252
  • /
  • 2023
  • /
  • 1225-4339(pISSN)
  • /
  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

갓과 흑미강 혼합물의 항산화 활성 및 지방세포 억제 효과

Antioxidant Activity and Anti-Obesity Effects of Mixture of Brassica juncea and Black Rice Bran

  • 임지현 (강원대학교 식품환경융합학과 ) ;
  • 임준석 (강원대학교 바이오산업공학부 식품생명공학전공 ) ;
  • 한웅호 (강원대학교 식품환경융합학과 ) ;
  • 문효 (강원대학교 식품환경융합학과 ) ;
  • 오건 (강원대학교 식품환경융합학과 ) ;
  • 조근희 (강원대학교 바이오산업공학부 식품생명공학전공 ) ;
  • 최선일 (강원대학교 농업생명과학연구원) ;
  • 황운상 ((주)에스티알 바이오텍 ) ;
  • 이옥환 (강원대학교 식품환경융합학과)
  • Ji-Heon Im (Dept. of Food Biotechnology and Environmental Science, Kangwon National University) ;
  • June seok Lim (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Xionggao Han (Dept. of Food Biotechnology and Environmental Science, Kangwon National University) ;
  • Xiao Men (Dept. of Food Biotechnology and Environmental Science, Kangwon National University) ;
  • Geon Oh (Dept. of Food Biotechnology and Environmental Science, Kangwon National University) ;
  • Geun hee Cho (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Sun-Il Choi (Agricultural and Life Sciences Research Institute, Kangwon National University) ;
  • Woonsang Hwang (STR Biotech Co., Ltd.) ;
  • Ok-Hwan Lee (Dept. of Food Biotechnology and Environmental Science, Kangwon National University)
  • 투고 : 2023.07.10
  • 심사 : 2023.07.28
  • 발행 : 2023.08.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study was conducted to provide basic data on the antioxidant activity, inhibition of adipocyte differentiation and reactive oxygen species (ROS) production of a mixture of Brassica juncea extract (BJE) and fermented black rice fraction (BRF). We investigated the total phenol content, total flavonoid content, antioxidant effects (DPPH radical scavenging, ABTS radical scavenging, reducing power, FRAP and ORAC assay) and anti-obesity activity of the mixture in 3T3-L1 cells. Our results showed that the total phenol and flavonoid content increased with increasing BRF mixture ratio. The antioxidant activity increased as the BRF mixture ratio increased. In addition, BJE and BRF mixtures did not show any cytotoxicity during the 3T3-L1 differentiation period. During adipocyte differentiation, BJE and BRF mixtures significantly inhibited lipid accumulation and ROS production compared to the control group. These results warrant further experiments to develop an anti-obesity functional food using a mixture of BJE and BRF.

키워드

과제정보

본 논문은 과학기술정보통신부와 연구개발특구진흥재단의 "연구개발특구육성(R&D, 2022-IT-RD-0236-01)" 사업의 지원 받아 수행된 연구로 이에 감사드립니다.

참고문헌

  1. Adan A, Kiraz Y, Baran Y. 2016. Cell proliferation and cytotoxicity assays. Curr Pharm Biotechnol 17:1213-1221 https://doi.org/10.2174/1389201017666160808160513
  2. Aranaz P, Navarro-Herrera D, Zabala M, Migueliz I, Romo-Hualde A, Lopez-Yoldi M, Martinez JA, Vizmanos JL, Milagro FI, Gonzalez-Navarro CJ. 2019. Phenolic compounds inhibit 3T3-L1 adipogenesis depending on the stage of differentiation and their binding affinity to PPARγ. Molecules 24:1045
  3. Ballinger A, Peikin SR. 2002. Orlistat: Its current status as an anti-obesity drug. Eur J Pharmacol 440:109-117 https://doi.org/10.1016/S0014-2999(02)01422-X
  4. Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 239:70-76 https://doi.org/10.1006/abio.1996.0292
  5. Cheigh CI, Chung EY, Ko MJ, Cho SW, Chang PS, Park YS, Lee KA, Paik HD, Kim KT, Hong SI, Chung MS. 2010. Effect of subcritical water for the enhanced extraction efficiency of polyphenols and flavonoids from black rice bran. Food Eng Prog 14:335-341
  6. Chen HC, Farese RV Jr. 2005. Inhibition of triglyceride synthesis as a treatment strategy for obesity: Lessons from DGAT1-deficient mice. Arterioscler Thromb Vasc Biol 25:482-486 https://doi.org/10.1161/01.ATV.0000151874.81059.ad
  7. Cheung BMY, Cheung TT, Samaranayake NR. 2013. Safety of antiobesity drugs. Ther Adv Drug Saf 4:171-181 https://doi.org/10.1177/2042098613489721
  8. Cho ML, Lee HS, Kang IJ, Won MH, You SG. 2011. Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed. Food Chem 127:999-1006 https://doi.org/10.1016/j.foodchem.2011.01.072
  9. Cho YS, Ha BS, Park SK, Chun SS. 1993. Contents of carotenoids and chlorophylls in dolsan leaf mustard (Brassica juncea). Korean J Diet Cult 8:153-157
  10. Choi H, Lee M, Kwak S Choi D. 2022. Antioxidant effect of tea tree root extracts using various extraction methods. Korean J Food Nutr 35:313-323
  11. Choi Y, Jeong HS, Lee J. 2007. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 103:130-138 https://doi.org/10.1016/j.foodchem.2006.08.004
  12. Chumchoochart W, Sutthanut K. 2020. Anti-obesity potential of glutinous black rice bran extract: Anti-adipogenesis and lipolysis induction in 3T3-L1 adipocyte model. Songklanakarin J Sci Technol 42:284-291
  13. Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, Nakayama O, Makishima M, Matsuda M, Shimomura I. 2004. Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest 114:1752-1761 https://doi.org/10.1172/JCI21625
  14. Ghasemzadeh A, Karbalaii MT, Jaafar HZE, Rahmat A. 2018. Phytochemical constituents, antioxidant activity, and anti-proliferative properties of black, red, and brown rice bran. Chem Cent J 12:17
  15. Halliwell B. 1996. Antioxidants in human health and disease. Annu Rev Nutr 16:33-50 https://doi.org/10.1146/annurev.nu.16.070196.000341
  16. Jun HI, Song GS, Yang EI, Youn Y, Kim YS. 2012. Antioxidant activities and phenolic compounds of pigmented rice bran extracts. J Food Sci 77:C759-C764 https://doi.org/10.1111/j.1750-3841.2012.02763.x
  17. Kim SP, Park SO, Lee SJ, Nam SH, Friedman M. 2014. A polysaccharide isolated from the liquid culture of Lentinus edodes (Shiitake) mushroom mycelia containing black rice bran protects mice against salmonellosis through upregulation of the Th1 immune reaction. J Agric Food Chem 62:2384-2391 https://doi.org/10.1021/jf405223q
  18. Kim YH, Lee YJ, Park SO, Lee SJ, Lee OH. 2013. Antioxidant compounds and antioxidant activities of fermented black rice and its fractions. Korean J Food Sci Technol 45:262-266 https://doi.org/10.9721/KJFST.2013.45.2.262
  19. Kwon HY, Choi SI, Park HI, Choi SH, Sim WS, Yeo JH, Cho JH, Lee OH. 2020. Comparative analysis of the nutritional components and antioxidant activities of different Brassica juncea cultivars. Foods 9:840
  20. Kwon HY, Choi SI, Han X, Men X, Jang GW, Choi YE, Lee OH. 2021. Antiobesity effect of Brassica juncea cultivated in Jeongseon with optimized sinigrin content using 3T3-L1 adipocytes. J Food Biochem 45:e13650
  21. Lee H, Lee YJ, Choi H, Ko EH, Kim J. 2009b. Reactive oxygen species facilitate adipocyte differentiation by accelerating mitotic clonal expansion. J Biol Chem 284: 10601-10609 https://doi.org/10.1074/jbc.M808742200
  22. Lee OH, Kwon YI, Hong HD, Park CS, Lee BY, Kim YC. 2009a. Production of reactive oxygen species and changes in antioxidant enzyme activities during differentiation of 3T3-L1 adipocyte. J Korean Soc Appl Biol Chem 52:70-75 https://doi.org/10.3839/jksabc.2009.012
  23. Lee OH, Seo MJ, Choi HS, Lee BY. 2012a. Pycnogenol® inhibits lipid accumulation in 3T3-L1 adipocytes with the modulation of reactive oxygen species (ROS) production associated with antioxidant enzyme responses. Phytother Res 26:403-411 https://doi.org/10.1002/ptr.3568
  24. Lee S, Na UJ, Choi SI, Han X, Men X, Lee YH, Kim HD, Kim YJ, Lee OH. 2021. Anti-obesogenic effect of Brassica juncea extract on bisphenol-A induced adipogenesis of 3T3-L1 cells. J Food Hyg Saf 36:528-536 https://doi.org/10.13103/JFHS.2021.36.6.528
  25. Lee YJ, Yoon BR, Choi HS, Lee BY, Lee OH. 2012b. Effect of Sargassum micracanthum extract on lipid accumulation and reactive oxygen species (ROS) production during differentiation of 3T3-L1 preadipocytes. Korean J Food Preserv 19:455-461 https://doi.org/10.11002/kjfp.2012.19.3.455
  26. Mason EE. 1992. Methods for voluntary weight loss and control. Obes Surg 2:275-276 https://doi.org/10.1381/096089292765560187
  27. McMurray F, Patten DA, Harper ME. 2016. Reactive oxygen species and oxidative stress in obesity-Recent findings and empirical approaches. Obesity 24:2301-2310 https://doi.org/10.1002/oby.21654
  28. Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71:109-114 https://doi.org/10.1016/S0378-8741(99)00189-0
  29. Na Y, Song YJ, Lee JJ. 2022. Quality characteristics and antioxidant effects of rice cookies enriched with dried plum (Prunus domestica L.) powder. Korean J Food Nutr 35: 499-512
  30. Ou B, Hampsch-Woodill M, Prior RL. 2001. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Agric Food Chem 49:4619-4626 https://doi.org/10.1021/jf010586o
  31. Safrida, Budijanto S, Nuraida L, Priosoeryanto BP, Saepuloh U, Marya SS, Ardiansyah, Shirakawa H. 2022. Fermented black rice bran extract inhibit colon cancer proliferation in WiDr cell lines. Food Sci Technol 42:e14422
  32. Slavin JL, Martini MC, Jacobs DR Jr, Marquart L. 1999. Plausible mechanisms for the protectiveness of whole grains. Am J Clin Nutr 70:459S-463S https://doi.org/10.1093/ajcn/70.3.459s
  33. Spiegelman BM, Flier JS. 2001. Obesity and the regulation of energy balance. Cell 104:531-543 https://doi.org/10.1016/S0092-8674(01)00240-9
  34. Talalay P, Fahey JW. 2001. Phytochemicals from cruciferous plants protect against cancer by modulating carcinogen metabolism. J Nutr 131:3027S-3033S https://doi.org/10.1093/jn/131.11.3027S
  35. Visscher TLS, Seidell JC. 2001. The public health impact of obesity. Annu Rev Public Health 22:355-375 https://doi.org/10.1146/annurev.publhealth.22.1.355
  36. Yamashita A, Soga Y, Iwamoto Y, Asano T, Li Y, Abiko Y, Nishimura F. 2008. DNA microarray analyses of genes expressed differentially in 3T3-L1 adipocytes co-cultured with murine macrophage cell line RAW264.7 in the presence of the toll-like receptor 4 ligand bacterial endotoxin. Int J Obes 32:1725-1729 https://doi.org/10.1038/ijo.2008.153
  37. Yen GC, Duh PD. 1993. Antioxidative properties of methanolic extracts from peanut hulls. J Am Oil Chem Soc 70:383-386 https://doi.org/10.1007/BF02552711
  38. Yoon BR, Cho BJ, Lee HK, Kim DJ, Rhee SK, Hong HD, Kim KT, Cho CW, Choi HS, Lee BY, Lee OH. 2012. Antioxidant and anti-adipogenic effects of ethanolic extracts from tartary and common buckwheats. Korean J Food Preserv 19:123-130 https://doi.org/10.11002/kjfp.2012.19.1.123