Mycobiology

The Korean Society of Mycology (한국균학회)
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The Cell Wall Integrity MAP Kinase Signaling Pathway Is Required for Development, Pathogenicity, and Stress Adaption of the Pepper Anthracnose Fungus Colletotrichum scovillei

  • Teng Fu (Division of Bio-Resource Sciences and Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Sung Wook Kang (Division of Bio-Resource Sciences and Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Yong-Won Song (Division of Bio-Resource Sciences and Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Kyoung Su Kim (Division of Bio-Resource Sciences and Interdisciplinary Program in Smart Agriculture, Kangwon National University)
  • Received : 2023.02.08
  • Accepted : 2023.05.18
  • Published : 2023.06.30
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Abstract

The cell wall integrity (CWI) signaling pathway plays important roles in the dissemination and infection of several plant pathogenic fungi. However, its roles in the pepper fruit anthracnose fungus Colletotrichum scovillei remain uninvestigated. In this study, the major components of the CWI signaling pathway-CsMCK1 (MAPKKK), CsMKK1 (MAPKK), and CsMPS1 (MAPK)-were functionally characterized in C. scovillei via homology-dependent gene replacement. The ΔCsmck1, DCsmkk1, and ΔCsmps1 mutants showed impairments in fungal growth, conidiation, and tolerance to CWI and salt stresses. Moreover, ΔCsmck1, ΔCsmkk1, and ΔCsmps1 failed to develop anthracnose disease on pepper fruits due to defects in appressorium formation and invasive hyphae growth. These results suggest that CsMCK1, CsMKK1, and CsMPS1 play important roles in mycelial growth, conidiation, appressorium formation, plant infection, and stress adaption of C. scovillei. These findings will contribute to a better understanding of the roles of the CWI signaling pathway in the development of pepper fruit anthracnose disease.

Keywords

Acknowledgement

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea grant (NRF-2020R1A2C100550700) funded by the Ministry of Education, Science and Technology, Republic of Korea. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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