Mycobiology

The Korean Society of Mycology (한국균학회)
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Neocucurbitaria chlamydospora sp. nov.: A Novel Species of the Family Cucurbitariaceae Isolated from a Stink Bug in Korea

  • Soo-Min Hong (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kallol Das (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Seong-Keun Lim (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Sang Jae Suh (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Seung-Yeol Lee (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Hee-Young Jung (College of Agriculture and Life Sciences, Kyungpook National University)
  • Received : 2023.01.30
  • Accepted : 2023.03.26
  • Published : 2023.06.30
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Abstract

The fungal strain KNUF-22-18B, belonging to Cucurbitariaceae, was discovered from a stink bug (Hygia lativentris) during the investigation of insect microbiota in Chungnam Province, South Korea. The colonies of the strain KNUF-22-18B were wooly floccose, white to brown in the center on oatmeal agar (OA), and the colonies were buff, margin even, and colorless, reverse white to yellowish toward the center on malt extract agar (MEA). The strain KNUF-22-18B produced pycnidia after 60 days of culturing on potato dextrose agar, but pycnidia were not observed on OA. On the contrary, N. keratinophila CBS 121759T abundantly formed superficial pycnidia on OA and MEA after a few days. The strain KNUF-22-18B produced chlamydospores subglobose to globose, mainly in the chain, with a small diameter of 4.4-8.8 ㎛. At the same time, N. keratinophila CBS 121759T displayed a globose terminal with a diameter of 8-10 ㎛. A multilocus phylogeny using the internal transcribed spacer regions, 28S rDNA large subunit, b-tubulin, and RNA polymerase II large subunit genes further validated the uniqueness of the strain. The detailed description and illustration of the proposed species as Neocucurbitaria chlamydospora sp. nov. from Korea was strongly supported by molecular phylogeny.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources, funded by the Ministry of Environment of the Republic of Korea [NIBR202231206].

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