Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Erysiphe lonicerigena sp. nov., a Powdery Mildew Species Found on Lonicera harae

  • In-Young Choi (Department of Agricultural Biology, Jeonbuk National University) ;
  • Lamiya Abasova (Department of Agricultural Biology, Jeonbuk National University) ;
  • Joon-Ho Choi (Department of Agricultural Biology, Jeonbuk National University) ;
  • Jung-Hee Park (Division of Biotechnology, College of Environmental & Bioresources Sciences, Jeonbuk National University) ;
  • Hyeon-Dong Shin (Division of Environmental Science and Ecological Engineering, Korea University)
  • Received : 2023.01.12
  • Accepted : 2023.01.23
  • Published : 2023.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

A powdery mildew (Erysiphaceae) has been continuously collected on the leaves of Lonicera harae in the southern part of the Korean Peninsula, where this shrub is indigenous. Microscopic examination of the asexual morphs revealed that the current collections are differentiated from the all known Erysiphe species on Lonicera spp. by its longer conidiophores and longer conidia. Although the morphology of the chasmothecia is reminiscent of Erysiphe ehrenbergii and E. lonicerae, the specimens on L. harae differ from them in having smaller ascospores. A phylogenetic tree generated from a combined dataset of the internal transcribed spacer region and 28S rDNA gene sequences demonstrates that sequences obtained from three powdery mildew collections on L. harae clustered together as an independent species clade with high bootstrap values distant from other Erysiphe species on Lonicera, representing a species of its own. Based on morphological differences and molecular-phylogenetic results, the powdery mildew on L. harae is proposed as a new species, Erysiphe lonicerigena, and the holomorph of the fungus is described and illustrated in this study.

Keywords

Acknowledgement

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Agriculture, Food and Rural Affairs Convergence Technologies Program for Educating Creative Global Leader Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) [Project no. 321001-03]. H.D. Shin was supported by a grant [K2123391] from Korea University, Korea.

References

  1. Mabberley DJ. Mabberley's plant-book: a portable dictionary of plants. 3rd ed. Avon: Cambridge University Press, 2008.
  2. Korean Society of Plant Pathology. List of plant diseases in Korea. 6th ed. Seoul, Korea: Korean Society of Plant Pathology; 2022.
  3. Bradshaw M, Braun U, Gotz M, et al. Taxonomy and phylogeny of the Erysiphe lonicerae complex (Helotiales, Erysiphaceae) on Lonicera spp. Fungal Syst Evol. 2021;7(1):49-65. https://doi.org/10.3114/fuse.2021.07.03
  4. Kim KH, Lee SK, Shin HD. First report of powdery mildew caused by Erysiphe lonicerae var. lonicerae on Lonicera sempervirens in Korea. Plant Pathol. 2008;57(2):374-374.
  5. Lee SH, Lee CK, Cho SE, et al. First report of powdery mildew caused by Erysiphe lonicerae var. lonicerae on Lonicera japonica in Korea. Plant Dis. 2016;100(4):856-856. https://doi.org/10.1094/PDIS-09-15-1031-PDN
  6. Choi IY, Abasova L, Choi JH, et al. First report of powdery mildew caused by Erysiphe lonicerae on Lonicera periclymenum in Korea. J Plant Pathol. 2022;104(3):1153-1153. https://doi.org/10.1007/s42161-022-01136-9
  7. Shin HD. Erysiphaceae of Korea. Suwon, Korea: National Institute of Agricultural Science and Technology; 2000.
  8. Son YH, Park SH, Jeong DH, et al. Growing environment characteristics and vegetation structure of Lonicera harae, medicinal plant. Korean J. Plant Res. 2021;34:297-310.
  9. Bradshaw M, Tobin PC. Sequencing herbarium specimens of a common detrimental plant disease (powdery mildew). Phytopathology. 2020;110(7):1248-1254. https://doi.org/10.1094/PHYTO-04-20-0139-PER
  10. Hall TA. BioEdit: a User-Friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95-98.
  11. Tamura K, Stecher G, Kumar S. MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol. 2021;38(7):3022-3027. https://doi.org/10.1093/molbev/msab120
  12. Swafford DL. PAUP: phylogenetic analyses using parsimony (and other methods) 4.0b10. Sunderland, MA: Sinauer; 2002.
  13. Braun U, Cook RTA. Taxonomic manual of the Erysiphales (powdery mildews). CBS biodiversity series. Utrecht, the Netherlands: CBS-KNAW Fungal Biodiversity Centre; 2012.
  14. Farr DF, Rossman AY. Fungal Databases, Systematic Mycology & Microbiology Laboratory, ARS, USDA. Available from: http://nt.ars-grin.gov/fungaldatabases/. Accessed 3 January 2023.
  15. Nakaji M, Tanaka N, Sugawara T. A molecular phylogenetic study of Lonicera L. (Caprifoliaceae) in Japan based on chloroplast DNA sequences. Acta Phytotaxonomica Geobotanica. 2015;6:137-151.