Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of a Salt Inducible Promoter from Chlorella vulgaris PKVL7422

  • Min-Jeong Kim (School of Marine and Fisheries Sciences, Pukyong National University) ;
  • Su-Hyun Kim (School of Marine and Fisheries Sciences, Pukyong National University) ;
  • Najib Abdellaoui (School of Marine and Fisheries Sciences, Pukyong National University) ;
  • Tae-Jin Choi (School of Marine and Fisheries Sciences, Pukyong National University)
  • Received : 2023.04.04
  • Accepted : 2023.04.20
  • Published : 2023.07.28
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Abstract

Chlorella is a eukaryotic organism that can be used as an industrial host to produce recombinant proteins. In this study, a salt-inducible promoter (SIP) was isolated from the freshwater species Chlorella vulgaris PKVL7422 from the screening of genes that were upregulated after salt treatment. Several cis-acting elements, including stress response elements, were identified in the isolated SIP. Moreover, the Gaussia luciferase gene was cloned after the SIP and transformed into C. vulgaris to test the inducibility of this promoter. Reexamination of transcriptome of C. vulgaris revealed that genes involved in the synthesis of methyl jasmonic acid (MeJA), gibberellin (GA), and abscisic acid (ABA) were upregulated when C. vulgaris was treated with salt. Furthermore, the expression level of recombinant luciferase increased when the transformed C. vulgaris was treated with salt and MeJA, GA, and ABA. This study represents the first report of the C. vulgaris SIP and highlights how transformed microalgae could be used for robust expression of recombinant proteins.

Keywords

Acknowledgement

This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF)& funded by the Korean government (MSIT) (No. 2022M3E5E6079747). This research was supported by Korea Institute of Marine Science & Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (20210292).

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