Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Effects of CTHRC1 on odontogenic differentiation and angiogenesis in human dental pulp stem cells

  • Jong-soon Kim (Department of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • Bin-Na Lee (Department of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • Hoon-Sang Chang (Department of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • In-Nam Hwang (Department of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • Won-Mann Oh (Department of Conservative Dentistry, School of Dentistry, Chonnam National University) ;
  • Yun-Chan Hwang (Department of Conservative Dentistry, School of Dentistry, Chonnam National University)
  • Received : 2023.01.04
  • Accepted : 2023.02.08
  • Published : 2023.05.31
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Abstract

Objectives: This study aimed to determine whether collagen triple helix repeat containing-1 (CTHRC1), which is involved in vascular remodeling and bone formation, can stimulate odontogenic differentiation and angiogenesis when administered to human dental pulp stem cells (hDPSCs). Materials and Methods: The viability of hDPSCs upon exposure to CTHRC1 was assessed with the WST-1 assay. CTHRC1 doses of 5, 10, and 20 ㎍/mL were administered to hDPSCs. Reverse-transcription polymerase reaction was used to detect dentin sialophosphoprotein, dentin matrix protein 1, vascular endothelial growth factor, and fibroblast growth factor 2. The formation of mineralization nodules was evaluated using Alizarin red. A scratch wound assay was conducted to evaluate the effect of CTHRC1 on cell migration. Data were analyzed using 1-way analysis of variance followed by the Tukey post hoc test. The threshold for statistical significance was set at p < 0.05. Results: CTHRC1 doses of 5, 10, and 20 ㎍/mL had no significant effect on the viability of hDPSCs. Mineralized nodules were formed and odontogenic markers were upregulated, indicating that CTHRC1 promoted odontogenic differentiation. Scratch wound assays demonstrated that CTHRC1 significantly enhanced the migration of hDPSCs. Conclusions: CTHRC1 promoted odontogenic differentiation and mineralization in hDPSCs.

Keywords

Acknowledgement

This research was funded by a Chonnam National University (GN: 2020-1833) and National Research Foundation of Korea grant funded by the Korean government (NRF-2020R1F1A1048271).

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