환경생물 (Korean Journal of Environmental Biology)

  • 제41권4호
  • /
  • Pages.345-363
  • /
  • 2023
  • /
  • 1226-9999(pISSN)
  • /
  • 2287-7851(eISSN)
한국환경생물학회 (Korean Society of Environmental Biology)
권호 표지
DOI QR코드

DOI QR Code

다유전자 분석을 통한 한국산 녹조류 Desmodesmus속의 계통

Phylogeny of Desmodesmus (Scenedesmaceae, Chlorophyceae) in Korea based on multigene data analysis

  • 유영채 (한국해양과학기술원 해양생태연구센터) ;
  • 이남주 (경기대학교 생명과학과) ;
  • 전가영 (한국해양과학기술원 해양생태연구센터) ;
  • 이옥민 (경기대학교 생명과학과) ;
  • 양은찬 (한국해양과학기술원 해양생태연구센터)
  • Yeong Chae Yoo (Marine Ecosystem Research Center, Korea Institute of Ocean Science & Technology) ;
  • Nam-Ju Lee (Department of Life Science, College of Natural Science, Kyonggi University) ;
  • Ga Yeong Jeon (Marine Ecosystem Research Center, Korea Institute of Ocean Science & Technology) ;
  • Ok-Min Lee (Department of Life Science, College of Natural Science, Kyonggi University) ;
  • Eun Chan Yang (Marine Ecosystem Research Center, Korea Institute of Ocean Science & Technology)
  • 투고 : 2023.08.08
  • 심사 : 2023.10.30
  • 발행 : 2023.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

뗏목말과 녹조류는 담수생태계 주요 우점생물군으로, 형태적 가변성과 종 구분 기준의 복잡성이 증가하고 있어 전 세계 40속 약 380종의 계통분류체계는 명확하지 않다(Comas and Komárek 1984; Kim 2015a). 우리나라의 뗏목말과는 17속 119종이 알려져 있으나, Desmodesmus 속 38종의 계통관계에 대한 연구는 전무하다. 본 연구 결과는 우리나라 뗏목말과 Desmodesmus속 종간 계통관계를 다유전자 분석을 기반으로 제시한다. 본 연구는 국립낙동강생물자원관 담수생물자원은행 (FBCC)이 보유하고 있는 Desmodesmus속 19종(unidentifies spp 제외) 70개 배양주의 총 299개 유전자(핵 18S rRNA, 색소체 atpB, petA, rbcL, 및 tufA) 서열을 분석하였다. 본 연구에서 사용한 Desmodesmus속 종의 생태 및 형태적 특징으로 유연관계를 추정하기는 매우 어려웠다. 그러나 색소체 유전자(atpB, petA, rbcL 및 tufA)는 핵 18S rRNA보다 높은 변이율(P-distance)을 보여주며 종간 계통관계 구축의 유용성을 보여주고 있다. 다유전자 유합계통수는 Desmodesmus속 내 6개 이상의 clades 구분을 제안하고 있다. Clade-1에는 D. serratus, unidentified Desmodesmus sp. 1 FBCC-A515, 및 unidentified Desmodesmus sp. 2 FBCC-A1236를 포함하며, Clade-2는 D. communis, D. dispar, D. maximus, D. pannonicus 및 unidentified Desmodesmus sp. 3 (FBCC-A724 와 FBCC-A725)를 포함한다. Clade-3는 D. bicaudatus와 D. intermedius의 2종을, Clade-4는 D. microspina, D. pleiomorphus, D. subspicatus, 및 unidentified Desmodesmus sp. 10 (FBCC-A1279 및 FBCC-A1332)의 4종을, Clade-5는 D. abundans, D. spinosus, unidentified Desmodesmus sp. 4 FBCC-A1293, 및 unidentified Desmodesmus sp. 5 (FBCC-A1277 등)의 4종을, Clade-6는 D. armatus, D. armatus var. longispina, D. opoliensis, unidentified Desmodesmus spp 6~9를 포함한다. 담수생물자원은행(FBCC) 배양주의 새로운 유전자 정보는 뗏목말과 녹조류의 종동정 및 분자생태학적 연구에 활용할 수 있다. 또한 분자계통 결과는 우리나라 Desmodesmus속 녹조류의 종다양성 정보 확대에 기여할 수 있다.

The genus Desmodesmus (Chodat) S.S. An, T. Friedl & E. Hegewald is ubiquitous in freshwater ecosystems, such as rivers, ponds, and wetlands. The actual species diversity and distribution of the genus is unknown because of morphological plasticity affected by habitats. Currently, 38 Desmodesmus species have been reported in Korea most of which transferred from the genus Scenedesmus recently, however, no phylogenetic relationships have been studied yet. Despite the challenges in analyzing relationships among Desmodesmus species through the morphology, ecology, and original description, this study focused on examining species-level relationships using the FBCC culture strains isolated from Korea. A total of 299 sequences (66 of 18S rRNA, 47 of atpB, 67 of petA, 52 of rbcL, and 67 of tufA) were newly determined and used for phylogenetic analysis. Four plastid genes tend to have higher variation than 18S rRNA in the variable sites and P-distance. From the combined phylogeny, the Desmodesmus included six clades such as Clade-1: D. pseudoserratus and D. serratus, Clade-2: D. communis, D. dispar, D. maximus, D. pannonicus, unidentified Desmodesmus sp., Clade-3: D. bicaudatus and D. intermedius, Clade-4: D. microspina, D. multivariablis, D. pleiomorphus, D. subspicatus, Clade-5: D. abundans, D. kissii, and D. spinosus, and Clade-6: D. armatus, D. armatus var. longispina, D. opoliensis, unidentified Desmodesmus spp. The new sequence data from FBCC strains will be used to identify species and study the molecular ecology of scenedesmacean green algae in freshwater ecosystems. The phylogenetic information from this study will expand our understanding of Desmodesmus species diversity in Korea.

키워드

과제정보

본 연구는 환경부의 재원으로, 한국환경산업기술원(KEITI) 다부처 국가생명연구자원 선진화 사업(MOE_No.20180430)의 지원으로 수행하였습니다.

참고문헌

  1. An SS, T Friedl and E Hegewald. 1999. Phylogenetic relationships of Scenedesmus and Scenedesmus-like coccoid green algae as inferred from ITS-2 rDNA sequence comparison. Plant Biol. 1:418-428. https://doi.org/10.1111/j.1438-8677.1999.tb00724.x
  2. Bang SD, JH Kim, BC Yim and OM Lee. 2018. Five taxa of newly recorded species of Scenedesmaceae(Sphaeropleales, Chlorophyceae, Chlorophyta) in Korea. Korean J. Environ. Biol. 36:271-276. https://doi.org/10.11626/KJEB.2018.36.3.271
  3. Comas A and J Komarek. 1984. Taxonomy and nomenclature of several species of Scenedesmus (Chlorellales). Arch. Hydrobiol.(Suppl. 67), Algol. Stud. 35:135-157.
  4. Guiry MD and GM Guiry. 2023. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. https://www.algaebase.org. Accessed July 10, 2023.
  5. Hegewald E. 1978. Eine neue Unterteilung der Gattung Scenedesmus Meyen. Nova Hedwigia 30:343-376. https://doi.org/10.1127/nova.hedwigia/30/1979/343
  6. Hegewald E. 2000. New combinations in the genus Desmodesmus (Chlorophyceae, Scenedesmaceae). Algol. Stud. 96:1-18. https://doi.org/10.1127/algol_stud/96/2000/1
  7. Hegewald E and P Silva. 1988. Annotated catalogue of Scenedesmus and nomenclaturally related genera including original descriptions and figures. Bibl. Phycol. 80:1-587.
  8. Hegewald E, M Wolf, A Keller, T Friedl and L Krienitz. 2010. ITS2 sequence-structure phylogeny in the Scenedesmaceae with special reference to Coelastrum (Chlorophyta, Chlorophyceae), including the new genera Comasiella and Pectinodesmus. Phycologia 49:325-335. https://doi.org/10.2216/09-61.1
  9. Hirose HM, T Akiyama, H Imahori, H Kasaki, S Kumano, H Kobayasi, E Takahashi, T Tsumura, M Hirano and T Yamagishi. 1977. Illustrations of the Japanese Freshwater Algae. Uchidarokakuhe. Tokyo, Japan.
  10. Jeon SL and E Hegewald. 2006. A revision of the species Desmodesmus perforatus and D. tropicus (Scenedesmaceae, Chlorophyceae, Chlorophyta). Phycologia 45:567-584. https://doi.org/10.2216/05-63.1
  11. John DM, BA Whitton and AJ Brook. 2011. The Freshwater Algal Flora of the British Isles. An Identification Guide to Freshwater and Terrestrial Algae. Second ed. Cambridge University Press. Cambirdge, United Kingdom.
  12. Johnson JL, MW Fawley and KP Fawley. 2007. The diversity of Scenedesmus and Desmodesmus (Chlorophyceae) in Itasca State Park, Minnesota, USA. Phycologia 46:214-229. https://doi.org/10.2216/05-69.1
  13. Kaufnerova V and M Elias. 2013. The demise of the genus Scotiellopsis Vinatzer (Chlorophyta). Nova Hedwigia 97:415-428. https://doi.org/10.1127/0029-5035/2013/0116
  14. Kim YJ. 2015a. Algal Flora of Korea. Freshwater Green Algae, Vol. 6. No. 8. Chlorophyta: Chlorophyceae: Chlorococcales III: Scenedesmaceae. National Institute of Biological Resources. Incheon, Korea.
  15. Kim YJ. 2015b. New records of genus Scenedesmus (Chlorophyceae) found in Korea. J. Ecol. Environ. 38:213-227. https://doi.org/10.5141/ecoenv.2015.022
  16. Komarek J and B Fott. 1983. Das phytoplankton des Susswassers. Chlorophyceae - Chlorococcales. In: Die Binnengewasser 16(7/1). Schweizerbart, Stuttgart.
  17. Lee OM and JH Kim. 2015. National List of Species of Korea 「Green Algae」. National Institute of Biological Resources. Incheon, Korea.
  18. Lewis LA. 1997. Diversity and phylogenetic placement of Bracteacoccus Tereg (Chlorophyceae, Chiorophyta) based on 18S ribosomal RNA gene sequence data. J. Phycol. 33:279-285. https://doi.org/10.1111/j.0022-3646.1997.00279.x
  19. Mai XC, CR Shen, CL Liu, DM Trinh and ML Nguyen. 2023. "DNA signaturing" database construction for Tetradesmus species identification and phylogenetic relationships of Scenedesmus-like green microalgae (Scenedesmaceae, Chlorophyta). J. Phycol. 59:775-784. https://doi.org/10.1111/jpy.13354
  20. Prescott GW. 1962. Algae of the Western Great Lakes Area. WM. C. Brown Co. Publisher. Dubuque, Iowa. https://doi.org/10.5962/bhl.title.4650
  21. Rambaut A. 2010. FigTree. V.1.4.4. Institute of Evolutionary Biology, University of Edinburgh. Edinburgh, Scotland. http://tree.bio.ed.ac.uk/software/figtree. Accessed October 27, 2023
  22. Shin HJ, MA Song and OM Lee. 2013. A study of nine newly reported species of the order Chlorococcales (Chlorophyta) in Hongcheon River, Korea. J. Ecol. Environ. 36:315-325. https://doi.org/10.5141/ecoenv.2013.315
  23. Stamatakis A. 2014. RaxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312-1313. https://doi.org/10.1093/bioinformatics/btu033
  24. Trainor FR. 1998. Biological aspects of Scenedesmus (Chlorophyceae) phenotypic plasticity. Nova Hedwigia 117:1-367.
  25. Trainor FR and PF Egan. 1990. Phenotypic plasticity in Scenedesmus (Chlorophyta) with special reference to S. armatus uniucells. Phycologia 29:461-469. https://doi.org/10.2216/i0031-8884-29-4-461.1
  26. Van Hannen E, P FinkGodhe and M Lurling. 2002. A revised secondary structure model for the internal transcribed spacer 2 of the green algae Scenedesmus and Desmodesmus and its implication for the phylogeny of these algae. Eur. J. Phycol. 37:203-208. https://doi.org/10.1017/S096702620200361X
  27. Zou S, C Fei, C Wang, Z Gao, Y Bao, M He and C Wang. 2016. How DNA barcoding can be more effective in microalgae identification: a case of cryptic diversity revelation in Scenedesmus (Chlorophyceae). Sci. Rep. 6:36822. https://doi.org/10.1038/srep36822