Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Flowering, Fertilization Characteristics and Speedy Breeding in a Protandry Plant Peucedanum japonicum Thunb.

웅예선숙 식물인 갯기름나물(식방풍)의 개화 특성 및 세대촉진 방법

  • Jin Tae Kim (Interdisciplinary Program in Agricultural Genomics, College of Agriculture & Life Sciences, Seoul National University) ;
  • Ho Jun Joh (Interdisciplinary Program in Agricultural Genomics, College of Agriculture & Life Sciences, Seoul National University) ;
  • Hong Seob Yu (Plant Genomics & Breeding Institute, Research Institute of Agriculture and Life Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Young Sang Park (Department of Agriculture, Forestry and Bioresources, College of Agriculture & Life Sciences, Seoul National University) ;
  • Ji-Seok Kim (Department of Agriculture, Forestry and Bioresources, College of Agriculture & Life Sciences, Seoul National University) ;
  • Yong Hyeok Jeong (Department of Agriculture, Forestry and Bioresources, College of Agriculture & Life Sciences, Seoul National University) ;
  • Tae-Jin Yang (Department of Agriculture, Forestry and Bioresources, College of Agriculture & Life Sciences, Seoul National University)
  • 김진태 (서울대학교 농업생명과학대학 유전체협동과정) ;
  • 조호준 (서울대학교 농업생명과학대학 유전체협동과정) ;
  • 유홍섭 (서울대학교 유전체육종연구소) ;
  • 박영상 (서울대학교 농업생명과학대학 농림생물자원학부) ;
  • 김지석 (서울대학교 농업생명과학대학 농림생물자원학부) ;
  • 정용혁 (서울대학교 농업생명과학대학 농림생물자원학부) ;
  • 양태진 (서울대학교 농업생명과학대학 농림생물자원학부)
  • Received : 2023.02.21
  • Accepted : 2023.06.13
  • Published : 2023.08.01
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Abstract

Peucedanum japonicum Thunberg is a biennial plant that usually flowers and dies after seed setting at 2nd year. We inspected the flowering and fertilization characteristics to establish the breeding system. The plant forms complex umbel inflorescences consisting of multiple umbellets and numerous flowers. Each flower is bisexual but allogamous because of protandry. A flower bloomed for three days with matured pollen and no stigma appearance. Inspection of pollen tube growth indicates that the pollen viability can be maintained for up to 3~7 days after first flowering (DAF). In a umbellet, flowers bloomed during 1~5 DAF and the pollen maintained viability during 3~10 DAF, and the pistil developed and maintained fertility during 8~12 DAF. Theoretically, self-pollination can be possible during 8~10 DAF in the same umbellet. Approximately 4.4% of seeds were developed by self-pollination among flowers in an enveloping solitary umbel. There is at least a 9~10 days difference for the development of an umbel with another umbel at the next branch, which indicates pollination can be permitted between different branches in an individual plant. Finally, we have established a rapid breeding method to shorten the breeding cycle to one year from two years by integrating the flowering and pollination characteristics.

본 연구는 갯기름나물의 개화특성 및 수정능력을 파악하여 육종의 기초자료로 이용하고자 수행되었다. 개화기 관찰 결과, 소화서 내 소화들은 바깥쪽에서부터 중앙쪽 방향으로 5일의 시차를 두고 개화하였다. 단일 소화는 웅예선숙형 특성을 가지며 개화와 더불어 화분은 5일간 유지되었고, 자예는 개화후 8일차 부터 발달하여 13일까지 임성을 유지하였다. 화분은 웅예출현 직후 2일째에 활력이 대폭 증가하였지만 하루만에 급감하였다. 동일화서 내 자가수정능력을 확인한 결과, 일부에서 자식성종자를 결실하였고, DNA 마커에 의해 자가수정 개체임이 확인되었다. 개체내 분지는 주경을 기점으로 영양상태에 따라 최대 4차지경까지 분지되며, 지경 화서 간 평균 8일 동안 자예 활성, 화분 활력시기가 중복되어 다른 가지의 화서간 자가수정이 가능하다고 판단된다. 노지에서 생육 후 12월 말까지 춘화처리 된 개체들을 12월 말에 온실내에서 생식생장을 유도하고 5월에 채종함으로써 개화시기를 약 4개월정도 단축시킬 수 있었다. 채종한 종자는 5~6월에 파종하고 영양생장 후 12월 말까지 춘화처리 후 5월에 채종할 수 있어 기존2년 1세대 육종기간을 1년 1세대로 단축할 수 있었다.

Keywords

Acknowledgement

본 논문은 농촌진흥청 연구사업(과제번호: PJ017134)의 지원에 의해 이루어진 결과로 이에 감사드립니다.

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