Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Anti-influenza Compounds Isolated from Descurainia sophia Seeds

  • Woo Seung Yang (Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University) ;
  • Choong Je Ma (Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University)
  • Received : 2023.03.02
  • Accepted : 2023.06.16
  • Published : 2023.06.30
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Abstract

Descurainia sophia seeds methanol extract showed significant anti-influenza activity and we tried to isolate anti-influenza compounds from the D. sophia extract. D. sophia seeds were extracted with 80% methanol and fractionated with n-hexane, ethyl acetate, CHCl3 and n-butanol. The anti-influenza activity of each fraction was assessed using sulforhodamine B (SRB) method in A549 cells, human-derived lung cancer cells. The ethyl acetate and CHCl3 fractions showed the most potent anti-influenza activity. Seven compounds were isolated from CHCl3 fraction and identified 1-decanol (1), 2-(3,4-dihydroxy-2-methylenebutoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (2), daucosterol (3), isorhamnetin (4), quercetin (5), sinapic acid (6), and helveticoside (7) by spectroscopic data such as UV, IR, 1H-NMR, 13C-NMR and mass spectroscopy. Anti-influenza activities of isolated compounds were evaluated using SRB method in A549 cells. Compounds 3, 4 and 7 had significant anti-influenza activity in a dose-dependent manner.

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References

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