Korean Journal of Biological Psychiatry (생물정신의학)

The Korean Society of Biological Psychiatry (대한생물정신의학회)
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Chronic Reserpine Administration for Depression Modeling in Zebrafish

레서핀 반복 투여를 통한 제브라피쉬 우울증 모델

  • Seyoung Kim (Department of Psychiatry, Korea University Ansan Hospital, Korea University College of Medicine) ;
  • Changsu Han (Department of Psychiatry, Korea University Guro Hospital, Korea University College of Medicine) ;
  • Young-Hoon Ko (Department of Psychiatry, Korea University Ansan Hospital, Korea University College of Medicine) ;
  • Yong-Ku Kim (Department of Psychiatry, Korea University Ansan Hospital, Korea University College of Medicine) ;
  • Ho-Kyoung Yoon (Department of Psychiatry, Korea University Ansan Hospital, Korea University College of Medicine) ;
  • Jongha Lee (Department of Psychiatry, Korea University Ansan Hospital, Korea University College of Medicine) ;
  • Suhyun Kim (Department of Biomedical Sciences, Korea University College of Medicine) ;
  • Chanhee Lee (Zebrafish Translational Medical Research Center, Korea University) ;
  • Cheolmin Shin (Department of Psychiatry, Korea University Ansan Hospital, Korea University College of Medicine)
  • 김세영 (고려대학교 안산병원 정신건강의학과) ;
  • 한창수 (고려대학교 구로병원 정신건강의학과) ;
  • 고영훈 (고려대학교 안산병원 정신건강의학과) ;
  • 김용구 (고려대학교 안산병원 정신건강의학과) ;
  • 윤호경 (고려대학교 안산병원 정신건강의학과) ;
  • 이종하 (고려대학교 안산병원 정신건강의학과) ;
  • 김수현 (고려대학교 의과대학 의과학과) ;
  • 이찬희 (고려대학교 안산병원 제브라피쉬 중개의학 연구소) ;
  • 신철민 (고려대학교 안산병원 정신건강의학과)
  • Received : 2022.11.14
  • Accepted : 2022.11.18
  • Published : 2023.04.30
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Abstract

Objectives This study aims to develop valid experimental models for depression through chronic reserpine exposure to zebrafish (Danio rerio). Methods The effect of chronic reserpine on zebrafish behavior in the novel tank was examined. Changes of gene expression on telencephalon were also investigated. Results Chronic reserpine (40 mg/L, 7 days) induced overt behavioral effects, but markedly reduced activity, resembling motor retardation in depression. In telencephalon of zebrafish, gene expression associated with monoamine oxidase and norepinephrine transporter was decreased. Expression of serotonin transporter gene was increased. Conclusions Our results show that the pharmacological model of depression in zebrafish can induce not only behavioral changes, but also monoamine changes in the homology of human mood regulation centers.

Keywords

Acknowledgement

This research was supported by a Korea University grant and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (grant number: 2019R1I1A1A01056594).

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