Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Draft Genome Sequence of Bacillus thuringiensis serovar aizawai AS23, Isolated from the Rhizosphere of Korean Melon (Cucumis melo L.)

  • Da-Ryung Jung (Department of Applied Biosciences, Kyungpook National University) ;
  • GyuDae Lee (Department of Applied Biosciences, Kyungpook National University) ;
  • Kyeongmo Lim (Department of Applied Biosciences, Kyungpook National University) ;
  • Yeonkyeong Lee (Department of Integrative Biology, Kyungpook National University) ;
  • Ga-Yeon Nam (Department of Applied Biosciences, Kyungpook National University) ;
  • Do-Yeun Won (Seongju Korean Melon Fruit and Vegetable Research Institute) ;
  • Na-Yun Park (Seongju Korean Melon Fruit and Vegetable Research Institute) ;
  • Young-Jin Seo (Seongju Korean Melon Fruit and Vegetable Research Institute) ;
  • Jae-Ho Shin (Department of Applied Biosciences, Kyungpook National University)
  • Received : 2023.10.05
  • Accepted : 2023.11.07
  • Published : 2023.12.28
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Abstract

We report the draft genome sequence of Bacillus thuringiensis serovar aizawai AS23, an insecticidal strain targeting lepidopteran pests, which was isolated from the rhizosphere of Korean melon (Cucumis melo L.). The genome of strain AS23 comprising 6,846,584 bp with a G + C content of 34.83% was assembled to 11 contigs obtained using hybrid assembly. Additionally, we mined the genome for pesticidal genes, identifying several insecticidal genes, including Cry1Aa3, Cry1Ca9, Cry1Da2, Cry1Ia44, Cry2Ab41, Cry9Ea9, Spp1Aa1, and Vip3Aa86.

Keywords

Acknowledgement

This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ017033)" Rural Development Administration, Republic of Korea.

References

  1. Sanahuja G, Banakar R, Twyman RM, Capell T, Christou P. 2011. Bacillus thuringiensis: a century of research, development and commercial applications. Plant Biotechnol. J. 9: 283-300. 
  2. Bravo A, Likitvivatanavong S, Gill SS, Soberon M. 2011. Bacillus thuringiensis: a story of a successful bioinsecticide. Insect Biochem. Mol. Biol. 41: 423-431. 
  3. Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, et al. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62: 775-806. 
  4. Bechtel Donald B, Lee A. Bulla Jr. 1976. Electron microscope study of sporulation and parasporal crystal formation in Bacillus thuringiensis. J. Bacteriol. 127: 1472-1481. 
  5. Hofte H, Whiteley HR. 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53: 242-255. 
  6. Zimin AV, Puiu D, Luo MC, Zhu T, Koren S, Marcais G, et al. 2017. Hybrid assembly of the large and highly repetitive genome of Aegilops tauschii, a progenitor of bread wheat, with the MaSuRCA mega-reads algorithm. Genome Res. 27: 787-792. 
  7. Chen KT, Liu CL, Huang SH, Shen HT, Shieh YK, Chiu HT, et al. 2018. CSAR: a contig scaffolding tool using algebraic rearrangements. Bioinformatics 34: 109-111. 
  8. Wick RR, Holt KE. 2022. Polypolish: short-read polishing of longread bacterial genome assemblies. PLoS Comput. Biol. 18: e1009802. 
  9. Hyatt D, Chen GL, LoCascio PF, Land ML, Larimer FW, Hauser LJ. 2010. Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinformatics 11: 119. 
  10. Crickmore N, Berry C, Panneerselvam S, Mishra R, Connor TR, Bonning BC. 2021. A structure-based nomenclature for Bacillus thuringiensis and other bacteria-derived pesticidal proteins. J. Invertebr. Pathol. 186: 107438.