Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Draft Genome Sequence of the Yeast Strain Hormonema macrosporum POB-4, which Produces the Biosurfactant Glycocholic Acid

  • Parthiban Subramanian (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jeong-Seon Kim (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jun Heo (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Yiseul Kim (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2023.10.05
  • Accepted : 2023.11.07
  • Published : 2023.12.28
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Abstract

We report the draft genome sequence of the yeast strain Hormonema macrosporum POB-4, capable of producing the biosurfactant glycocholic acid, one of the bile acids. A majority of genes with known function were associated with metabolism and transport of amino acid and carbohydrate as well as secondary metabolites biosynthesis, transport, and catabolism. We observed genes of eleven C-N hydrolases and two CoA transferases which have been reported to be involved in the biosynthesis of glycocholic acid. Further experimental studies can help to elucidate the specific genes responsible for biosurfactant production in strain POB-4.

Keywords

Acknowledgement

This study (Project No. 015675) was carried out with the support of National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

References

  1. Ernst C, Rahmann S. 2013. PanCake: A Data Structure for Pangenomes. German Conference on Bioinformatics.
  2. Vaser R, Sovic I, Nagarajan N, Sikic M. 2017. Fast and accurate de novo genome assembly from long uncorrected reads. Genome Res. 27: 737-746. https://doi.org/10.1101/gr.214270.116
  3. Manni M, Berkeley MR, Seppey M, Simao FA, Zdobnov EM. 2021. BUSCO Update: novel and streamlined workflows along with broader and deeper phylogenetic coverage for scoring of eukaryotic, prokaryotic, and viral genomes. Mol. Biol. Evol. 38: 4647-4654. https://doi.org/10.1093/molbev/msab199
  4. Stanke M, Diekhans M, Baertsch R, Haussler D. 2008. Using native and syntenically mapped cDNA alignments to improve de novo gene finding. Bioinformatics 24: 637-644. https://doi.org/10.1093/bioinformatics/btn013
  5. Cantalapiedra CP, Hernandez-Plaza A, Letunic I, Bork P, Huerta-Cepas J. 2021. eggNOG-mapper v2: Functional annotation, orthology assignments, and domain prediction at the metagenomic scale. Mol. Biol. Evol. 38: 5825-5829. https://doi.org/10.1093/molbev/msab293
  6. Xie C, Mao X, Huang J, Ding Y, Wu J, Dong S, et al. 2011. KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases. Nucleic Acids Res. 39: W316-W322. https://doi.org/10.1093/nar/gkr483
  7. Blin K, Shaw S, Augustijn HE, Reitz ZL, Biermann F, Alanjary M, et al. 2023. antiSMASH 7.0: new and improved predictions for detection, regulation, chemical structures and visualisation. Nucleic Acids Res. 51: W46-W50.
  8. Pil GB, Won HS, Shin HJ. 2016. Bile acids from a marine sponge-associated fungus Penicillium sp. J. Korean Magnetic Reson. Soc. 20: 41-45. https://doi.org/10.6564/JKMRS.2016.20.2.041
  9. Ohashi K, Miyagawa Y, Nakamura Y, Shibuya H. 2008. Bioproduction of bile acids and the glycine conjugates by Penicillium fungus. J. Nat. Med. 62: 83-86. https://doi.org/10.1007/s11418-007-0190-3
  10. Maneerat S, Nitoda T, Kanzaki H, Kawai F. 2005. Bile acids are new products of a marine bacterium, Myroides sp. strain SM1. Appl. Microbiol. Biotechnol. 67: 679-683. https://doi.org/10.1007/s00253-004-1777-1
  11. Kim D, Lee JS, Kim J, Kang SJ, Yoon JH, Kim WG, et al. 2007. Biosynthesis of bile acids in a variety of marine bacterial taxa. J. Microbiol. Biotechnol. 17: 403-407.
  12. Garcia CJ, Kosek V, Beltran D, Tomas-Barberan FA, Hajslova J. 2022. Production of new microbially conjugated bile acids by human gut microbiota. Biomolecules 12: 687.