Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Valorization of Pineapple Peel Waste for Sustainable Polyhydroxyalkanoates Production

  • Kannika Bunkaew (Microbial Technology for Agriculture, Food and Environment Research Center, Faculty of Science, Thaksin University) ;
  • Kittiya Khongkool (Microbial Technology for Agriculture, Food and Environment Research Center, Faculty of Science, Thaksin University) ;
  • Monthon Lertworapreecha (Microbial Technology for Agriculture, Food and Environment Research Center, Faculty of Science, Thaksin University) ;
  • Kamontam Umsakul (Division of Biological Sciences, Faculty of Science, Prince of Songkla University) ;
  • Kumar Sudesh (Ecobiomaterial Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia) ;
  • Wankuson Chanasit (Microbial Technology for Agriculture, Food and Environment Research Center, Faculty of Science, Thaksin University)
  • Received : 2023.05.30
  • Accepted : 2023.07.31
  • Published : 2023.09.28
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Abstract

The potential polyhydroxyalkanoates (PHA)-producing bacteria, Bacillus megaterium PP-10, was successfully isolated and studied its feasibility for utilization of pineapple peel waste (PPW) as a cheap carbon substrate. The PPW was pretreated with 1% (v/v) H2SO4 under steam sterilization and about 26.4 g/l of total reducing sugar (TRS) in pineapple peel hydrolysate (PPH) was generated and main fermentable sugars were glucose and fructose. A maximum cell growth and PHA concentration of 3.63 ± 0.07 g/l and 1.98 ± 0.09 g/l (about 54.58 ± 2.39%DCW) were received in only 12 h when grown in PPH. Interestingly, PHA productivity and biomass yield (Yx/s) in PPH was about 4 times and 1.5 times higher than in glucose. To achieve the highest DCW and PHA production, the optimal culture conditions e.g. carbon to nitrogen ratios of 40 mole/mole, incubation temperature at 35℃ and shaking speed of 200 rpm were performed and a maximum DCW up to 4.24 ± 0.04 g/l and PHA concentration of 2.68 ± 0.02 g/l (61% DCW) were obtained. The produced PHA was further examined its monomer composition and found to contain only 3-hydroxybutyrate (3HB). This finding corresponded with the presence of class IV PHA synthase gene. Finally, certain thermal properties of the produced PHA i.e. the melting temperature (Tm) and the glass transition temperature (Tg) were about 176℃ and -4℃, respectively whereas the Mw was about 1.07 KDa ; therefore, the newly isolated B. megaterium PP-10 is a promising bacterial candidate for the efficient conversion of low-cost PPH to PHA.

Keywords

Acknowledgement

This work was financially supported by National Higher Education, Science, Research and Innovation Policy Council, Thaksin University (Research project grant no. TSU-65A105000021) Fiscal Year 2022.

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