Journal of Microbiology and Biotechnology

  • 제33권12호
  • /
  • Pages.1543-1551
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  • 2023
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Comparative Analysis of T4SS Molecular Architectures

  • Mishghan Zehra (Department of Biochemistry, University of Missouri) ;
  • Jiwon Heo (Department of Biotechnology, The Catholic University of Korea) ;
  • Jeong Min Chung (Department of Biotechnology, The Catholic University of Korea) ;
  • Clarissa L Durie (Department of Biochemistry, University of Missouri)
  • 투고 : 2023.07.06
  • 심사 : 2023.07.24
  • 발행 : 2023.12.28
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초록

The recently published high-resolution R388 T4SS structure provides exciting new details about the complete complex of T4SS, including the components making up the stalk and arches, numerous symmetry mismatches between regions of the complex, and an intriguing interpretation of the closed stalk and radial symmetry of the inner membrane complex, which is related to pilus biogenesis assembly. However, there are a few unidentified densities in the electron microscopy map and portions of the identified component sequences for which the structure is not yet known. It is also unclear how well this minimized DNA-transporting T4SS predicts the structure of other T4SSs, such as expanded systems and those that transport proteins rather than DNA. In this review, we evaluate what can be inferred from the recent high-resolution structure of the R388 T4SS with respect to the Cag and Dot/Icm systems. These systems were selected because, given what is currently known about these systems, we expect them to present most structural differences compared to the R388 T4SS structure. Furthermore, we discuss bacterial physiology and diversity, the T4SS structures and their variations between different bacterial species. These insights may prove beneficial for researchers who elucidate the structure and functions of T4SS in different bacterial species.

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과제정보

This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2022R1C1C1003352) and the Catholic University of Korea, Research Fund, 2022.

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