Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Immune enhancing activity of Sargassum horneri extracts via MAPK pathway in macrophages

대식세포에서 괭생이모자반 추출물의 MAPKs 기전 통한 면역활성 증가 효과

  • 김동섭 (한국프라임제약(주)) ;
  • 김민지 (한국프라임제약(주)) ;
  • 성낙윤 (한국프라임제약(주)) ;
  • 한인준 (한국프라임제약(주)) ;
  • 김건 (한국프라임제약(주)) ;
  • 김춘성 (조선대학교 치과대학 구강생화학) ;
  • 유영춘 (건양대학교 의과대학 미생물학교실) ;
  • 정윤우 (한국프라임제약(주))
  • Received : 2023.02.23
  • Accepted : 2023.05.22
  • Published : 2023.06.30
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Abstract

Sargassum horneri (SH), a brown macroalgae, has medicinal properties. The present study investigated the immune-enhancing effects of SH extract on peritoneal macrophages (PM). The SH significantly increased the production of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and nitric oxide (NO) in PM. It was confirmed that SH significantly increased NO expression through the increase of iNOS protein expression, which is the up-regulation pathway. Additionally, it was determined if SH activates the mitogen-activated protein kinase (MAPK) pathway, an upper regulatory mechanism that influences TNF-α, IL-6, and NO expression. Consequently, SH significantly increased the phosphorylation of p38, extracellular signal-regulated kinases (ERK), and c-Jun N-terminal kinase (JNK), all of which are MAPK pathway proteins. Moreover, the immune-enhancing effects of SH on another macrophage cell line, bone marrow-derived macrophages were investigated. It was observed that SH significantly enhanced TNF-α, IL-6, and NO production. Overall, this study demonstrates the immune-enhancing effects of SH on macrophages via activated MAPK pathway. Therefore, it suggests that SH has the potential to improve immunological activity in various macrophage cell lines and can be useful as an immune-enhancing treatment.

Keywords

Acknowledgement

이 논문은 2021년도 해양수산부 재원으로 해양수산과학기술진흥원의 지원을 받아 수행된 연구임. (20210656, 빅데이터 기반 바이오헬스케어 유효성 소재 실증 지원)

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