International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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The complete mitochondrial genome of the blue-tailed damselfly Ischnura elegans (Odonata: Coenagrionidae)-a climate-sensitive indicator species in South Korea

  • Seung Hyun Lee (Department of Applied Biology, College of Agriculture & Life Sciences, Chonnam National University) ;
  • Jeong Sun Park (Department of Applied Biology, College of Agriculture & Life Sciences, Chonnam National University) ;
  • Jee-Young Pyo (Department of Applied Biology, College of Agriculture & Life Sciences, Chonnam National University) ;
  • Sung-Soo Kim (Research Institute for East Asian Environment and Biology) ;
  • Iksoo Kim (Department of Applied Biology, College of Agriculture & Life Sciences, Chonnam National University)
  • Received : 2023.04.03
  • Accepted : 2023.05.31
  • Published : 2023.06.30
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Abstract

The blue-tailed damselfly, Ischnura elegans Van der Linden, 1820 (Odonata: Coenagrionidae), is a climate-sensitive indicator species in South Korea. In this study, we sequenced the complete mitochondrial genome (mitogenome) of I. elegans collected from South Korea for subsequent population genetic analysis, particularly to trace population movements in response to climate change. The 15,963 base pair (bp)-long complete mitogenome of I. elegans has typical sets of genes including a major non-coding region (the A+T-rich region), and an arrangement identical to that observed in ancestral insect species. The ATP6, ND3 and ND1 genes have the TTG start codon, which, although rare, is the canonical start codon for animal mitochondrial tRNA. The A/T content was 71.4% in protein-coding genes, 72.1% in tRNAs, 72.9% in the whole genome, 74.7% in srRNA, 75.3% in lrRNA, and 83.8% in the A+T-rich region. The A+T-rich region is unusually long (1,196 bp) and contains two subunits (192 bp and 176-165 bp), each of which is tandemly triplicated and surrounded by non-repeat sequences. Comparison of the sequence divergence among available mitogenomes of I. elegans, including the one from the current study, revealed ND2 as the most variable gene, followed by COII and COI, suggesting that ND2 should be targeted first in subsequent population-level studies. Phylogenetic reconstruction based on all available mitogenome sequences of Coenagrionidae showed a strong sister relationship between I. elegans and I. senegalensis.

Keywords

Acknowledgement

This study was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry through the Agriculture, Food, and Rural Affairs Convergence Technologies Program and Educating Creative Global Leader Program, funded by the Ministry of Agriculture, Food, and Rural Affairs (Grant no. 321001-03).

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