DOI QR코드

DOI QR Code

계절 변화에 따른 한국산 가시오가피 줄기의 페놀성 성분 함량 분석

Seasonal Variation of Phenolic Component Contents in the Stems of Korean Acanthopanax senticosus

  • 김도형 (세명대학교 한방식품영양학과) ;
  • 고성권 (세명대학교 한방식품영양학과) ;
  • 양병욱 (세명대학교 바이오제약산업학부)
  • Do Hyeong Kim (Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Sung Kwon Ko (Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Byung Wook Yang (School of Industrial Bio-Pharmaceutical Science, Semyung University)
  • 투고 : 2023.05.05
  • 심사 : 2023.06.21
  • 발행 : 2023.06.30

초록

Objectives: The aim of this study was to compare the total phenolic content of Korean Acanthopanax senticosus stems harvested in different seasons and provide basic data for developing functional reinforcement products based on the optimal harvesting time. Methods: Each sample harvested in different seasons was extracted and concentrated twice for 2 hours using 70% ethyl alcohol. Phenolic compounds were analyzed using high-performance liquid chromatography for simultaneous multi-component analysis of 14 compounds, including syringaresinol and so on. Results: The results showed that the stem of Korean Acanthopanax senticosus harvested in winter (November 29th) (EAS-5) had the highest phenolic content of 1.038%. The stem of Korean Acanthopanax senticosus harvested in autumn (October 1st) (EAS-4) showed the second-highest phenolic content of 0.764%, followed by the stem of Korean Acanthopanax senticosus harvested in spring (February 2nd) (EAS-1) with a content of 0.390%. On the other hand, the stem of Korean Acanthopanax senticosus harvested in the summer (June 2nd) (EAS-3) showed the lowest content at 0.342%. In conclusion, the stem of Korean Acanthopanax senticosus harvested in winter (EAS-5) showed the highest phenolic compound content. Conclusions: Considering the extraction yield and the total phenolic content, as well as the concentrations of key functional components such as eleutheroside B, chlorogenic acid, and syringaresinol in the 70% ethyl alcohol extract of Korean Acanthopanax senticosus, it is suggested that the stems of Korean Acanthopanax senticosus harvested during the winter season are suitable for the development of novel materials with enhanced anti-obesity functionality.

키워드

과제정보

본 연구는 농림축산식품부 미래형혁신식품기술개발사업(No. 119023-3)의 지원에 의해 이루어진 것임.

참고문헌

  1. Jo BS, Cho YJ. Biological activity of extracts from Acanthopanax sessiliflorum fruit. Korean J Food Preserv. 2012 ; 19(4) : 586-93. https://doi.org/10.11002/kjfp.2012.19.4.586
  2. Lee CB. A primary color Korea plant picture. Hymoonsa. 2003 : 812.
  3. Kim YM. Pharmaceutical comparison of Ginseng with Acanthopanax from the aspect of oriental medicine. Kor J Pharmacog. 1977 ; 8( 3) : 131-8.
  4. Kim CW, Lee HY. Studies on the constituents of seeds of Acanthopanax senticosus for inermis harms. Kor J Pharmacog. 1990 ; 21( 3) : 235-8.
  5. Heo SJ, Ahn HY, Kang MJ, Lee JH, Cha JY, Cho YS. Antioxidative activity and chemical characteristics of leaves, roots, stems and fruits extracts from Acanthopanax senticosus. J Life Sci. 2011 ; 21(7) : 1052-9. https://doi.org/10.5352/JLS.2011.21.7.1052
  6. Kim GY, Kim KY, Jeong HW. Immunostimulating effects of Acanthopanax in mice following Gamma-ray irradiation. J Physiol & Pathol Korean Med. 2006 ; 20(3) : 670-4.
  7. Wagner H, Heur YH, Obermeier A, Tittel G, Bladt S. Die DC-and HPLC-analyse der Eleutherococcus droge. J Med Plant Res. 1982 ; 44(4) : 193-8. https://doi.org/10.1055/s-2007-971445
  8. Nishibe S, Kinoshita H, Takeda H, Okano G. Phenolic compounds from stem bark of Acanthopanax senticosus and their pharmacological effect in chronic swimming stressed rats. Chem Pharm Bull. 1990 ; 38(6) : 1763-5. https://doi.org/10.1248/cpb.38.1763
  9. Fujikawa T, Yamaguchi A, Morita I, Takeda H, Nishibe S. Protective effects of Acanthopanax senticosus harms from Hokkaido and Its components on gastric ulcer in restrained cold water stressed rats. Biol Pharm Bull. 1996 ; 19(9) : 1227-30. https://doi.org/10.1248/bpb.19.1227
  10. Han SB, Yoon YD, Ahn HJ, Lee HS, Lee CW, Yoon WK, et al. Toll-like receptor-mediated activation of B cells and macrophages by polysaccharide isolated from cell culture of Acanthopanax senticosus. Int Immunopharmacol. 2003 ; 3( 9) : 1301-12. https://doi.org/10.1016/S1567-5769(03)00118-8
  11. Fujikawa T, Soya H, Hibasami H, Kawashima H, Takeda H, Nishibe S, et al. Effect of Acanthopanax senticosus harms on niogenic monoamine levels in the rat brain. Phytother Res. 2002 ; 16(5) : 474-8. https://doi.org/10.1002/ptr.1024
  12. Liu SM, LI XZ, Huo Y, Lu F. Protective effect of extract of Acanthopanax senticosus harms on dopaminergic neurons in Parkinson's disease mice. Phytomedicine. 2012 ; 19( 7) : 631-8. https://doi.org/10.1016/j.phymed.2012.02.006
  13. Kim SK, Kim YG, Lee MK, Han JS, Lee JH, Lee HY. Comparison of biological activity according to extracting solvents of four Acanthopanax root bark. Korean J Med Crop Sci. 2000 ; 8(1) : 21-8.
  14. Sung MS, Jung HY, Choi JH, Lee SC, Choi BH, Park SS. Preparation of functional healthy drinks by Acanthopanax senticosus extracts. J Life Sci. 2014 ; 24(9) : 959-66. https://doi.org/10.5352/JLS.2014.24.9.959
  15. Kuo J, Chen KW, Cheng IS, Tsai PH, Lu YJ, Lee NY. The effect of eight weeks of supplementation with Eleutherococcus senticosus on endurance capacity and metabolism in human. Chin J Physiol. 2010 ; 53(2) : 105-11. https://doi.org/10.4077/CJP.2010.AMK018
  16. Huang LZ, Huang BK, Ye Q, Qin LP. Bioactivity-guided fractionation for antifatigue property of Acanthopanax senticosus. J Ethnopharmacol. 2011 ; 133(1) : 213-9. https://doi.org/10.1016/j.jep.2010.09.032
  17. Zhang XL, Ren F, Huang W, Ding RT, Zhou QS, Liu XW. Anti-fatigue activity of extracts of stem bark from Acanthopanax senticosus. Molecules. 2011 ; 16(1) : 28-37. https://doi.org/10.3390/molecules16010028
  18. Jung HJ, Park HJ, Kim RG, Shin KM, Ha J, Choi JW, et al. In vivo anti-inflammatory and antinoceptive effects of lirodendrin isolated from the stem bark of Acanthopanax senticosus. Planta Med. 2003 ; 69(7) : 610-6. https://doi.org/10.1055/s-2003-41127
  19. Kim SD, Lee SI, Shin KO. Effect of Acanthopanax senticosus extracts of blood sugar and serum lipid profiles of streptozotocin-induced diabetic rats. J East Asian Soc Diet Life. 2005 ; 15( 5) : 549-57.
  20. Logan AC, Wong C. Chronic fatigue syndrome: oxidative stress and dietary modifications. Altern Med Rev. 2001 ; 6(5) : 450-9.
  21. Lee E, Choi MY, Park HJ, Cha BC, Cho SH. Chemical constituents and biological activity of kalopanacis cortex. Kor J Pharmacog. 1995 ; 26(2) : 122-9.
  22. Kim BB, Chang KH. Suppression of fat accumulation and improvement of glucose tolerance in high-fat diet-induced obese mice treated with an Acanthopanax senticosus extract. Kor J Pharmacog. 2015 ; 46(1) : 65-71.
  23. Park YH, Kim HY, Lim SH, Kim KH, Lee JH, Kim YG, et al. Effects of ethanol extract from leaves of Eleutherococcus senticosu on hyperlipidemia in rats. J Korean Soc Food Sci Nutr. 2012 ; 41( 3) : 333-8. https://doi.org/10.3746/jkfn.2012.41.3.333
  24. Park SH, Lee SG, Kang KS, Chung SH. Acanthopanax senticosus reverses fatty liver disease and hyperglycemia in ob/ob mice. Arch Pharm Res. 2006 ; 29(9) : 768-76. https://doi.org/10.1007/BF02974078
  25. Nishida M, Kondo M, Shimizu T, Saito T, Sato S, Hirayama M, et al. Antihyperlipidemic effect of Acanthopanax senticosus (Rupr. et Maxim) harms leaves in high-fat-diet fed mice. J Sci Food Agric. 2016 ; 96(11) : 3717-22. https://doi.org/10.1002/jsfa.7557
  26. Kim YH, Bae DB, Park SO, Lee SJ, Cho OH, Lee OH. Method validation for the determination of Eleutherosides and β-glucan in Acanthopanax koreanum. J Korean Soc Food Sci Nutr. 2013 ; 42(9) : 1419-25. https://doi.org/10.3746/jkfn.2013.42.9.1419
  27. Jeong JE, Baek HE, Oh DS, Wi AJ, Yoon BS. Functional properties and biological activity of breeding lines, parts, and various solvents from Acanthopanax. Korean J Pharmacogn. 2013 ; 44(3) : 242-52.
  28. Brekhman II, Dardymov IV. New substances of plant origin which increase nonspecific resistance. Annu Rev Pharmacol. 1969 ; 9 : 419-30.
  29. Hahn DR, Kim CJ, Kim JH. A study on the chemical constituents of Acanthopanax koreanum Nakai and its pharmaco biological activities. Yakhak Hoeji. 1985 ; 29( 6) : 357-61.
  30. Zhao L, An Q, Qin F, Xiong Z. Simultaneous determination of six constituents in the fruit of Acanthopanax sessiliflorus (Rupr. et Maxim.) seem by HPLC-UV. Nat Prod Res. 2014 ; 28( 7) : 500-2. https://doi.org/10.1080/14786419.2013.877904
  31. Choi SM, Park JB, Kim JM, In KM, Park HY. Acanthopanax senticosus extract acts as an important regulator for vascular functions. J Life Sci. 2008 ; 18(5) : 701-7. https://doi.org/10.5352/JLS.2008.18.5.701
  32. Lee KH, Yoon WH. Effects of protein-bound polysacharide isolated from Acanthopananx senticosus in reducing the toxic effects of cisplatin. Kor J Pharmacog. 2007 ; 38(2) : 152-6.