Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Toxic effect in olive flounder Paralichthys olivaceus after intramuscular administration of amprolium hydrochloride

Amprolium hydrochloride 투여에 따른 넙치 (Paralichthys olivaceus)의 독성 영향

  • Sang Hyup Park (Department of Aquatic Life Medicine, Gangneung-Wonju National University) ;
  • Young Kwang Choi (Department of Aquatic Life Medicine, Gangneung-Wonju National University) ;
  • Jeong-wan Do (Pathology Division, National Institute of Fisheries Science) ;
  • Hye-Sung Choi (Pathology Division, National Institute of Fisheries Science) ;
  • Yi Kyung Kim (Department of Aquatic Life Medicine, Gangneung-Wonju National University)
  • 박상협 (강릉원주대학교 수산생명의학과) ;
  • 최영광 (강릉원주대학교 수산생명의학과) ;
  • 도정완 (국립수산과학원 병리연구과) ;
  • 최혜승 (국립수산과학원 병리연구과) ;
  • 김이경 (강릉원주대학교 수산생명의학과)
  • Received : 2023.04.26
  • Accepted : 2023.06.08
  • Published : 2023.06.30
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Abstract

The aim of this study was to examine the effect of amprolium hydrochloride on olive flounder Paralichthys olivaceus. Thirty flounder (average weight 230.27 g; average length 27.99 cm) was randomly allocated into six groups, namely, 50, 100, 150, 200, 400 and 600 mg/kg. As measure of acute toxicity the LD50 value of 148.18 mg/kg was obtained. Hematologic analysis was performed on days 1, 3, 7, 14, and 28 after the intramuscular injection of the drug. Among the plasma biochemistry profiles, glutamic oxalate transaminase (GOT) activity and blood urea nitrogen (BUN) were found significantly increased. In addition, histopathology analysis revealed frequent appearance of melanomacrophage center (MMC) and edema in the renal tissues after 20, 40 and 80 mg/kg on day 7, 14, 28. In summary, amprolium exposure seem to cause adverse effects of flounders.

본 연구에서는 넙치(Paralichthys olivaceus, average weight 230.27 g; average length 27.99 cm)에서 amprolium hydrochloride의 주사 투여에 따른 급성독성을 평가하기 위해 50, 100, 150, 200, 400, 600 mg/kg 용량으로 설정하고 단회주사투여한 결과 LD50 값은 148.18 mg/kg이었다. 또한 약물을 주사투여한 후 1, 3, 7, 14, 28일째에 혈액학적 분석을 실시한 결과 GOT 수치는 80 mg/kg 용량구간에서만 대조구에 비해 1일차 및 3일차까지 최고수치를 나타내었으며, BUN 수치는 1일과 3일차 80 mg/kg 용량구간과 7일차에 40 및 80 mg/kg 구간에서 대조구에 비해 유의적인 증가를 나타내었다. 추가적으로 조직학적 변화를 확인한 결과 7, 14, 28일차, 20, 40, 80 mg/kg 용량 구간에서 신장 조직에서 melanomacrophage center와 부종이 관찰되었다. 결과적으로 amprolium hydrochloride 주사 투여는 넙치에서 독성을 나타내기 때문에 넙치의 생체 내 실험에서 여러가지 악영향을 미치는 것으로 보아 상당한 독성이 있는 것으로 판단된다.

Keywords

Acknowledgement

이 논문은 국립수산과학원(P2023202, 넙치쿠도아충 저감화 연구)의 지원에 의해 진행되었음.

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