Abstract
In this study, we aimed to compare the mycelial growth of Pleurotus ostreatus after medium supplementation with various amino acids at different concentrations to select the optimal medium nutrient composition for mycelial growth. The mycelial growth of P. ostreatus was investigated after adding four amino acids (tryptophan, threonine, methionine, and lysine) at 0.5% or 1% to the medium.The rate of P. ostreatus mycelial growthwas faster in the potato dextrose agar (PDA) medium supplemented with threonine at 0.5% or 1% than that of the control, whereas it was inhibited by tryptophan treatment. Supplementation of sawdust mediumwith all amino acids, except tryptophan, at 0.5% did not alter the mycelial growth, compared to the controls. However, addition of any amino acid to sawdust medium at a higher concentration (1%) inhibited the mycelial growth. The laccase acitivity of P. ostreatus mycelium cultured in PDA medium was the highest when threonine was added, and the lowest when tryptophane was added, consistent with the results of the mycelial growth. Therefore, the addition of threonine, methionine, or lysine to PDA medium at a concentration of 0.5-1%was effective for increasing the mycelial growth of P. ostreatus; however, it inhibited mycelial growth insawdust medium, suggesting that the effects of amino acids dependedon the medium nutrient composition.
아미노산 4종류(threonine, methionine, lysine, tryptophan)를 0.5%와 1.0%를 배지에 첨가하여 느타리 균사생장을 분석하였다. PDA 배지의 느타리 균사체 생장은 무첨가 대비 threonine 0.5, 1.0%에서 생육이 우수하였으며, tryptophan 0.5%, 1.0% 첨가 배지는 생장이 억제되었다. 톱밥 배지에서 아미노산 0.5% 첨가는 tryptophan을 제외하고 균사체 생장이 동등하였고, 4종류 아미노산 1.0% 첨가시 균사생장이 억제되었다. PDA 배지에서 배양한 균사체 laccase 활성은 threonine 첨가에서 가장 높았으며, tryptophan 첨가는 가장 낮아 균사체 배양 결과와 일치하였다. PDA 배지에 tryptophan을 제외한 threonine, methionine, lysine 0.5%와 1.0% 첨가는 느타리 균사체 생장에 효과적이었고, 톱밥 배지는 아미노산 1.0% 첨가가 억제효과를 나타낸 것은, 배지의 영양조건에 따라 아미노산 첨가 효과가 다르게 나타난 것으로 판단된다.