Journal of the Korean Wood Science and Technology

  • 제51권3호
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  • Pages.157-172
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  • 2023
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  • 1017-0715(pISSN)
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  • 2233-7180(eISSN)
한국목재공학회 (The Korean Society of Wood Science & Technology)
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Anti-Termite Activity of Azadirachta excelsa Seed Kernel and Its Isolated Compound against Coptotermes curvignathus

  • Morina ADFA (Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Bengkulu) ;
  • Khafit WIRADIMAFAN (Master of Chemistry Study Program, Faculty of Mathematics and Natural Sciences, University of Bengkulu) ;
  • Ricky Febri PRATAMA (Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Bengkulu) ;
  • Angga SANJAYA (Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University) ;
  • Deni Agus TRIAWAN (Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Bengkulu) ;
  • Salprima YUDHA S. (Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Bengkulu) ;
  • Masayuki NINOMIYA (Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University) ;
  • Mohamad RAFI (Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University) ;
  • Mamoru KOKETSU (Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University)
  • 투고 : 2022.12.18
  • 심사 : 2023.03.01
  • 발행 : 2023.05.25
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초록

Azadirachta excelsa, is a plant belonging to the same genus as Indian neem (Azadirachta indica), and its use as a pesticide is reported by few studies. Despite being a different species, it is expected to have the same biopesticide potential as A. indica. Therefore, this study aims to investigate the anti-termite activity of n-hexane and methanol extracts of A. excelsa seed kernel at various concentrations against Coptotermes curvignathus. The methanol extract demonstrated greater termicidal activity than n-hexane at doses test of 2%, 4%, and 8%. It also showed 100% termite mortality on the third day of administering the 8% dose. According to the gas chromatography with mass spectrometry data, the putative main components of the n-hexane extract were hexadecanoic acid, ethyl ester (18.99%), 9,12-octadecadienoic acid (Z,Z)- (16.31%), and 9-octadecenal (16.23%). In contrast, the principal constituents of methanol extract were patchouli alcohol (28.1%), delta-guaiene (15.15%), and alpha-guaiene (11.93%). Furthermore, limonoids profiling of A. excelsa methanol extract was determined using Ultrahigh-performance liquid chromatography coupled with quadrupole-Orbitrap high-resolution mass spectrometry. The number of limonoids identified tentatively was fifteen, such as 6-deacetylnimbin, nimbolidin C, nimbolide, 6-acetylnimbandiol, 6-deacetyl-nimbinene, salannol, 28-deoxonimbolide, gedunin, nimbandiol, epoxyazadiradione, azadirone, 2',3'-dihydrosalannin, marrangin, nimbocinol, and azadirachtin. They were the same as those reported in the seed and leaves of A. indica, but its largest component in A. excelsa was 6-deacetylnimbin. As a result, the presence of these compounds may be responsible for the anti-termite activity of A. excelsa seed kernel extract. Additionally, column chromatography of methanol extract yielded 6-deacetylnimbin, which was found to be antifeedant and termiticide against C. curvignathus.

키워드

과제정보

The authors are grateful to the Ministry of Education, Culture, Research, and Technology of the Republic of Indonesia (PDKN Grant No. 105/E5/PG.02.00.PT/2022), for financial and in-kind support from Koketsu Lab.

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