해양환경안전학회지 (Journal of the Korean Society of Marine Environment & Safety)

  • 제29권1호
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  • Pages.36-43
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  • 2023
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  • 1229-3431(pISSN)
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  • 2287-3341(eISSN)
해양환경안전학회 (The Korean Society of Marine Environment and safety)
권호 표지
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발광다이오드(LED) 파장에 따른 Tetraselmis suecica와 T. tetrathele의 탄수화물, 단백질 및 지질 함량에 미치는 영향

Effects of Light Quality of a Light-Emitting Diode (LED) on Carbohydrate, Protein, and Lipid Contents of Tetraselmis suecica and T. tetrathele

  • 한경하 (한국해양과학기술원 해양시료도서관) ;
  • 오석진 (부경대학교 지구환경시스템과학부)
  • Kyong Ha Han (Library of Marine Samples, Korea Institute of Ocean Science & Technology) ;
  • Seok Jin Oh (Division of Earth and Environmental System Science Pukyong National University)
  • 투고 : 2023.01.20
  • 심사 : 2023.02.24
  • 발행 : 2023.02.28
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초록

본 연구는 Tetraselmis suecica와 T. tetrathele의 영양물질 증진효과를 가져올 수 있는 배양시스템 구축을 위하여, 발광다이오드(LED)의 파장별(청색; 450 nm, 황색; 590 nm, 적색; 630 nm) 탄수화물, 단백질, 지질 함량을 측정하였다. 두 종 모두 단백질 비율(42~69%)이 가장 높았으며, 생장속도가 낮았던 황색파장에서 탄수화물, 단백질, 지질의 높은 함량을 보였고, 생장속도가 가장 높았던 적색파장에서는 낮은 함량을 보였다. 이러한 결과는 세포 분열 속도의 감소로 인해 단백질 합성과 함께 세포의 화학적 조성과 효소 활동에 변화를 주어 지질과 탄수화물 함량이 증가한 것으로 생각된다. 따라서, T. suecica와 T. tetrathele의 유용한 생화학적 물질의 증대를 위해 대수생장기 초기와 중기는 적색 LED 그리고 대수생장기 후기에는 황색 LED를 주사하는 2단계 LED 배양을 제안하였다.

To establish a culture system with enhanced cellular nutrition, we investigated the effects of light quality (blue, 450 nm; yellow, 590 nm; and red, 630 nm) of a light-emitting diode (LED) on the biochemical composition of Tetraselmis suecica and T. tetrathele. The protein content of both species was higher (42-69%) than the content of other biochemical substances under all wavelengths. Carbohydrate, protein, and lipid contents were higher under the yellow wavelength, which showed a low growth rate, than those under other wavelengths. The contents of all biochemical substances were low under the red wavelength, which showed a high growth rate. These results indicated that protein synthesis occurs in response to decreased cell division rate, while lipid and carbohydrate synthesis occurs owing to altered chemical composition and enzymatic activity. Therefore, we suggested a two-phase LED culture system, which emitted red LED during the early-middle exponential phase and yellow LED during the late exponential and stationary phases, to increase the yield of useful biochemical substances of T. suecica and T. tetrathele.

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과제정보

이 논문은 부경대학교 자율창의학술연구비(2021년)에 의하여 연구되었습니다.

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