생물환경조절학회지 (Journal of Bio-Environment Control)

  • 제32권2호
  • /
  • Pages.165-171
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  • 2023
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  • 1229-4675(pISSN)
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  • 2765-3641(eISSN)
한국생물환경조절학회 (The Korean Society for Bio-Environment Control)
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감자 맹아억제제 Chlorpropham의 마른썩음병 억제 효과

Inhibitory Effect of Potato Sprouting Inhibitor Chlorpropham on Dry Rot

  • 한규석 (강원도농업기술원 감자연구소) ;
  • 김병섭 (강릉원주대학교 식물생명과학과) ;
  • 홍세진 (강릉원주대학교 식물생명과학과) ;
  • 김남숙 (강릉원주대학교 식물생명과학과)
  • Kyusuk Han (Potato Experiment Station, Gangwondo Agricultural Research and Extension Services) ;
  • Byung Sup Kim (Department of Plant Science, Gangneung-Wonju National University) ;
  • Sae Jin Hong (Department of Plant Science, Gangneung-Wonju National University) ;
  • Nam Sook Kim (Department of Plant Science, Gangneung-Wonju National University)
  • 투고 : 2023.03.14
  • 심사 : 2023.04.26
  • 발행 : 2023.04.30
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초록

감자 마른썩음병은 Fusarium 종에 의해 발병하는 감자 저장병 중 하나이며 저장중 수확후 손실을 일으키는 대표적인 병리장해이다. 맹아억제를 위해 허용된 chlorpropham 처리는 주로 가공용 감자의 상온저장 시 이용되고 있다. 본 연구에서는 감자 저장 중 맹아의 출현을 억제하기 위해 사용되는 chlorpropham 처리가 Fusarium에 의한 마른썩음병(Fusarium solani Appel & Wollenw) 발생의 억제 효과가 있는지 확인하기 위해 감자 '단오' 품종을 이용하여 수행되었다. 마른썩음병균의 균사 생장률을 조사하기 위해균사 생장은 F. oxysporum 균사를 치상한 chlorpropham(5.0, 50.4, 503.8, 5,038ppm)과 prochloraz(0.1, 1.0, 10.0, 100.0ppm) 약제배지를 이용하여 in vitro에서 조사하였다. 20℃에서 14일간 치상한 균사는 chlorpropham과 prochloraz 처리 시 처리 농도가 높을수록 생장이 더욱 억제되었으며, 억제율은 chlorpropham 503.8ppm과 prochloraz 10ppm 처리 시 각각98.2%와 100%를 나타냈다. 20℃ 상온과 4℃ 저온저장 중 마른썩음병 방제 효과는 F. oxysporum을 접종한 감자 괴경을 chlorpropham(5.0, 50.4, 503.8ppm)과 prochloraz(100ppm)에 침지처리하여 조사하였다. 상온 저장한 감자(20℃)의 병반 직경은 저장 70일 때에 무처리구 괴경의 13.7mm에 비해 chlorpropham 50.4ppm 처리구에서만10.7mm로 유의성이 있게 억제되었다. 저온에서는 모든 처리구에서 병반 진행은 통계적 유의차가 없이 유사하였다. 본 연구 결과에서 저장 감자의 마른썩음병을 억제하기 위해서는 수확 후 chlorpropham 50.4ppm 처리가 유용할 것으로 판단된다.

Potato dry rot is one of the potato storage diseases caused by Fusarium species and is a representative pathological disorder that induced post-harvest loss during storage. Chlorpropham treatment for sprouting inhibition is mainly used for room temperature storage of potatoes for processing. In this study, the inhibitory effect of chlorpropham on Fusarium-induced dry rot of potato 'Dano'. To investigate the mycelial growth rate of the dry rot fungus (Fusarium solani Appel & Wollenw), mycelial growth was investigated in a chlorpropham (5.0, 50.4, 503.8, and 5,038 ppm) and prochloraz (0.1, 1.0, 10.0, and 100.0 ppm) medium containing F. oxysporum mycelia. Mycelia were more inhibited as the concentration of chlorpropham and prochloraz increased during incubation at 20℃, and the inhibition rate was 98.2% and 100% when treated with 503.8 ppm of chlorpropham and 10ppm of prochloraz in 14 days, respectively. Potato Dano tubers inoculated with F. oxysporum were dipped in chlorpropham (5.0, 50.4, and 503.8 ppm) and prochloraz (100 ppm) to investigate the effect of preventing dry rot during cold storage at 20℃ and 4℃ in vivo. The disease diameter of potatoes stored at room temperature (about 20℃) was reduced to 13.0 mm in the prochloraz 100 ppm teatment, and 10.7 mm in the chlorpropham 50.4 ppm treatment compared to 13.7 mm in the control tuber at 70 days of storage. The disease progression in all treatments including control was similar with no statistically significant difference at 4℃ air temperature. From the results of this study, it is considered that treatment with 50.4 ppm of chlorpropham after harvest will be useful for suppressing dry rot of stored potatoes.

키워드

과제정보

본 연구는 2021년도 농촌진흥청 연구개발과제(과제번호: PJ01560603)의 지원에 의해 이루어진 '고위도 지역 적응 감자 수확 후 관리 및 이용성 증진 연구' 과제로 수행되었습니다.

참고문헌

  1. Bojanowski A., T.J. Avis, S. Pelletier, and R.J. Tweddell 2013, Management of potato dry rot. Postharvest Biol Technol 84:99-109. https://doi.org/10.1016/j.postharvbio.2013.04.008
  2. Chelkowski J. 1989, Toxigenicity of Fusarium species causing dry rot of potato tubers. In J Chelkowski, ed, Fusarium: Mycotoxins, Taxonomy and Pathogenicity. Elsevier Science Publishing, New York, USA, pp 435-440.
  3. Corsini D., G. Stallknecht, and W. Sparks 1979, Changes in chlorpropham residues in stored potatoes. Am Potato J 56:43-50. doi:10.1007/BF02851122
  4. Daami-Remadi M. 2012, Potato Fusarium dry rot in Tunisia: Current status and future prospects. Pest Technol 6:15-22.
  5. Daami-Remadi M., and M.E. Mahjoub 2006, Presence en Tunisie d'isolats de Fusarium sambucinum resistants aux benzimidazoles : developpement in vitro et agressivite sur tubercules de pomme de terre. Biotechnol Agron Soc Environ 10:7-16.
  6. Daniels-Lake B.J., K. Pruski, and R.K. Prange 2011, Using ethylene gas and chlorpropham potato sprout inhibitors together. Potato Res 54:223-236. https://doi.org/10.1007/s11540-011-9188-z
  7. Elsherbiny E.A., B.H. Amin, and Z.A. Baka 2016, Efficiency of pomegranate (Punica granatum L.) peels extract as a high potential natural tool towards Fusarium dry rot on potato tubers. Postharvest Biol Technol 111:256-263. https://doi.org/10.1016/j.postharvbio.2015.09.019
  8. Environment Protection Agency (EPA) 1996, R.E.D. Facts, Chlorpropham. United States Environment Protection Agency, Washington, D.C., USA.
  9. Hanson L.E., S.J. Schwager, and R. Loria 1996, Sensitivity to thiabendazole in Fusarium species associated with dry rot of potato. Phytopathology 86:378-384. https://doi.org/10.1094/Phyto-86-378
  10. Hide G.A., and G.R. Cayley 1985, Effects of delaying fungicide treatment of wounded potatoes on the incidence of Fusarium dry rot in store. Ann Appl Biol 107:429-438. doi:10.1111/j.1744-7348.1985.tb03159.x
  11. Hide G.A., P.J. Read, and S.M. Hall 1992, Resistance to thiabendazole in Fusarium species isolated from potato tubers affected by dry rot. Plant Pathol 41:745-748. doi:10.1111/j.1365-3059.1992.tb02558.x
  12. Kim J.W. 2000, Plant Pathology. Daegu University Press, Daegu, Korea. (in Korean)
  13. Lee Kim M.S., E.E. Ewing, and J.B. Sieczka 1972, Effects of chlorpropham (CIPC) on sprouting of individual potato eyes and on plant emergence. Am Potato J 49:420-431. https://doi.org/10.1007/BF02861645
  14. Lee M.S. 1970, Effects of isopropyl AT-(3-chlorophenyl) carbamate on sprouting and metabolism of potato tubers. PhD Dissertation, Cornell University, NY, USA.
  15. Mahajan B.V.C., A. Dhatt, K. Sandhu, and A. Garg 2008, Effect of CIPC (isopropyl-N (3-chlorophenyl) carbamate) on storage and processing quality of potato. J Food Agric Environ 6:34-38.
  16. National Crop Pest Management System (NCPMS) 2023, Dry rot of potato. Available via https://ncpms.rda.go.kr/npms/ImageSearchInfoR1.np?detailKey=D00000075 (in Korean)
  17. Olofsson B. 1970, Chemicals and methods to inhibit sprouting during storage of potatoes. Medd St VaxtskAnst 14:323-340.
  18. Olsen N., J. Miller, and P. Nolte 2006, Diagnosis & management of potato storage diseases. University of Idaho Extension, Idaho Agricultural Experiment Station, ID, USA.
  19. Park J.S. 1988, Plant Pathology. Hyangmoonsa, Seoul, Korea. (in Korean)
  20. Paul V., R. Ezekiel, and R. Pandey 2016, Sprout suppression on potato: need to look beyond CIPC for more effective and safer alternatives. J Food Sci Technol 53:1-18. doi:10.1007/s13197-015-1980-3
  21. Rural Development Administration (RDA) 2012, Agricultural science and technology research and analysis standards, RDA, Suwon, Korea, p 475.
  22. Rural Development Administration (RDA) 2022, Chlorpropham, Pesticides safety information system, Available via https://psis.rda.go.kr/psis/agc/res/agchmRegistStusLst.ps
  23. Theron D.J., and G. Holz 1991, Prediction of potato dry rot based on the presence of Fusarium in soil adhering to tubers at harvest. Plant Dis 75:126-130. https://doi.org/10.1094/PD-75-0126
  24. Tiwari R.K., R. Kumar, S. Sharma, V. Sagar, R. Aggarwal, K.C. Naga, M.K. Lal, K.N. Chourasia, D. Kumar, and M. Kumar 2020, Potato dry rot disease: current status, pathogen-omics and management. 3 Biotech 10:503 doi:10.1007/s13205-020-02496-8
  25. Vaughn K.C., and L.P. Lehnen Jr. 1991, Mitotic disruptor herbicides. Weed Sci 39:450-457. https://doi.org/10.1017/S0043174500073215
  26. Wharton P., R. Hammerschmidt, and W. Kirk 2007, Fusarium dry rot. Michigan potato diseases series. Michigan State University, MI, USA.