한국균학회지 (The Korean Journal of Mycology)

  • 제51권1호
  • /
  • Pages.63-68
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  • 2023
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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Incidence of Beet Leaf Spot Caused by Neocamarosporium betae in Korea

  • 투고 : 2023.01.03
  • 심사 : 2023.03.09
  • 발행 : 2023.03.31
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초록

From June to August 2021, we surveyed diseases affecting beet (Beta vulgaris subsp. vulgaris) plants in Cheolwon, Hoengseong, and Pyeongchang regions in Gangwon Province, Korea. We observed severe leaf spot symptoms, such as brown to dark circular or irregular spots on the leaves, in plants. Disease incidence in the plant leaves in the fields investigated at the three locations ranged from 1 to 80%. Five single-spore isolates of Phoma sp. were obtained from the diseased leaves and identified as Neocamarosporium betae based on their cultural, morphological, and molecular characteristics. Three isolates of N. betae were subsequently tested to confirm their pathogenicity in beet plants via artificial inoculation. The tested isolates caused leaf spot symptoms in the inoculated plants, similar to those observed in the plants in the investigated fields. Therefore, our findings revealed N. betae as the pathogen causing beet leaf spot in Korea.

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과제정보

This study was supported by a research grant (PJ014507012021) from the Rural Development Administration, Korea.

참고문헌

  1. Plants of the World Online. Beta vulgaris subsp. vulgaris. [Internet]. Kew: Royal Botanic Garden; 2022 [cited 2022 December 28]. Available from https://powo.science.kew.org/. 
  2. Lee GB, Mo YM, Cho WD, Kim WG. Leaf spot of rhubarb caused by Didymella rhei in Korea. Kor J Mycol 2021;49:555-60. 
  3. Boerema GH, de Gruyter J, Noordeloos ME, Hamers MEC. Phoma identification manual. Differentiation of Specific and Infra-specific Taxa in Culture. Oxfordshire: CABI;2004. 
  4. Chen Q, Jiang, JR, Zhang GZ, Cai L, Crous PW. 2015. Resolving the Phoma enigma. Stud Mycol 2015;82:137-217.  https://doi.org/10.1016/j.simyco.2015.10.003
  5. Ariyawansa HA, Thambugala KM, Manamgoda DS, Jayawardena RS, Camporesi E, Boonmee S, Wanasinghe DN, Phookamsak R, Hongsanan S, Singtripop C, et al. Towards a natural classification and backbone tree for Pleosporaceae. Fungal Divers 2015;71:85-139.  https://doi.org/10.1007/s13225-015-0323-z
  6. Rehner SA, Samuels GJ. Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycol Res 1994;98:625-34.  https://doi.org/10.1016/S0953-7562(09)80409-7
  7. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990;172:4238-46.  https://doi.org/10.1128/jb.172.8.4238-4246.1990
  8. Sung G-H, Sung J-M, Hywel-Jones NL. A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): Identification of localized incongruence using a combinational bootstrap approach. Mol Phylogenet Evol 2007;44:1204-23.  https://doi.org/10.1016/j.ympev.2007.03.011
  9. Liu YJ, Whelen S, Hall BD. Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Mol Phylogenet Evol 1999;16:1799-808.  https://doi.org/10.1093/oxfordjournals.molbev.a026092
  10. Park M-J, Lee H, Ryoo R, Jang Y, Ka K-H. A rapid and universal direct PCR method for macrofungi. Kor J Mycol 2021;49:455-67. 
  11. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870-4.  https://doi.org/10.1093/molbev/msw054
  12. Bassimba DDM, Mira JL, Vicent A. First report of leaf spot of spinach caused by Pleospora betae in Spain. Plant Dis 2014;98:1583. 
  13. Chand N, Jones EE, Casonato S. Pathogenicity of Phoma betae isolates from red beet (Beta vulgaris) at seed farms in Canterbury, New Zealand. N Z Plant Prot 2019;72:21-6.  https://doi.org/10.30843/nzpp.2019.72.272
  14. Garibaldi A, Gilardi G, Bertetti D, Gullino ML. First report of leaf spot and root rot caused by Phoma betae on Beta vulgaris subsp. vulgaris (garden beet group) in Italy. Plant Dis 2007;91:1515. 
  15. Pool VW, McKay MB. Phoma betae on the leaves of the sugar beet. J Agric Res 1915;4:169-77. 
  16. List of Plant Diseases in Korea. [Internet]. Seoul: Korean Society of Plant Pathology; 2022. [cited 2022 December 28]. Available from http://genebank.rda.go.kr/kplantdisease.do.