Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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A Study on the Effectiveness of In Vitro as a Cosmetic Material of Sericin Originated from Silkworm Kochi

누에고치에서 유래된 세리신(Sericin)의 화장품 소재로서 In Vitro 효능 연구

  • Hyun-Ji Kwon (Advanced Translational Medicine, Konkuk University) ;
  • Jihye Lee (Daegu Haany University Industry Academic Cooperation Foundation) ;
  • Eun-mi Jung (Department of Pharmaceutical Engineering, Daegu haany University) ;
  • Eunhong Lee (Department of Pharmaceutical Engineering, Daegu haany University) ;
  • Jinhan Park (Division of Biotechnology and Convergence, College of Cosmetics and Pharm, Daegu Haany University) ;
  • Sung-Eum Oh (Garam of Nature) ;
  • Sanghyeok Park (Garam of Nature) ;
  • Ji Wook Jung (Division of Biotechnology and Convergence, College of Cosmetics and Pharm, Daegu Haany University)
  • 권현지 (건국대학교 일반대학교원 첨단중개의학과) ;
  • 이지혜 (대구한의대학교 산학협력단) ;
  • 정은미 (대구한의대학교 일반대학원 제약공학과) ;
  • 이은홍 (대구한의대학교 일반대학원 제약공학과) ;
  • 박진한 (대구한의대학교 화장품제약대학 바이오산업융합학부) ;
  • 오성음 (가람오브네이쳐) ;
  • 박상혁 (가람오브네이쳐) ;
  • 정지욱 (대구한의대학교 화장품제약대학 바이오산업융합학부)
  • Received : 2023.01.12
  • Accepted : 2023.02.26
  • Published : 2023.02.28
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Abstract

Sericin is a protein extracted from the cocoon and has many health benefits. This study was conducted to evaluate the anti-wrinkle and anti-inflammatory activity of sericin derived from cocoon as a cosmetic material. The antioxidant activity of sericin was measured by DPPH and ABTS assays. In addition, cell viability was confirmed in Raw 264.7 cells, which are macrophages, and the anti-inflammatory effect of sericin was investigated using the inflammatory response induced using lipopolysaccharide (LPS). It shows that sericin has antioxidant activity and can be an excellent material for anti-aging and anti-inflammatory cosmetics.

세리신은 누에고치에서 추출한 단백질로 많은 건강상의 이점을 가지고 있다. 본 연구는 화장품 소재로서 누에고치에서 유래된 세리신 표준품의 항주름 활성 및 항염증 활성을 평가하기 위해 수행되었다. 세리신의 항산화 효과는 DPPH 및 ABTS 측정법에 의해 측정되었다. 또한 대식세포인 Raw 264.7 cell에서의 세포 생존율을 확인하였으며, lipoplyscaccharide를 이용하여 유도된 염증반응을 이용하여 세리신의 항염증 효과를 조사하였다. 그 결과 세리신은 DPPH, ABTS에서 항산화 활성을 보였으며 세포 독성을 가지지 않은 1,000 ㎍/mL의 농도에서 NO를 억제하였다. 종합하여 세리신은 항산화 활성을 가지며 항노화 및 항염증 화장품의 우수한 소재가 될 수 있음을 보여준다.

Keywords

Acknowledgement

본 연구는 2022년도 경상북도 및 경북테크노파크의 바이오 코스메틱 생태계 조성사업의 지원을 받아 수행한 연구입니다(과제번호-코스메틱22-01).

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