한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제40권4호
  • /
  • Pages.856-867
  • /
  • 2023
  • /
  • 1225-9098(pISSN)
  • /
  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
권호 표지
DOI QR코드

DOI QR Code

미세먼지 PM10으로 손상을 유도한 피부각질형성세포에서 한약재 추출물의 피부 개선 효능 평가

Evaluation of skin improvement efficacy of herbal medicine extracts on skin keratinocytes stimulated with fine dust PM10

  • 김동희 (한국한의약진흥원) ;
  • 강윤환 (한국한의약진흥원) ;
  • 김보애 (목원대학교 화장품공학과)
  • Dong-Hee Kim (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Yun Hwan Kang (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Bo-Ae Kim (Department of Cosmetic Engineering, College of Technology Sciences, Mokwon University)
  • 투고 : 2023.08.04
  • 심사 : 2023.08.30
  • 발행 : 2023.08.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

환경오염에 의한 미세먼지의 증가로 피부는 산화적 손상과 노화가 가속화된다. 본 연구에서는 선발된 한약재 추출물의 항산화, hyaluronic acid, filaggrin, MMP-1, ROS 항목을 평가함으로써 PM10으로 부터의 각질형성세포 보호 효능을 확인하였다. 그 결과 1,1-diphenyl-2-picrylhydrazyl(DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid(ABTS), FRAP assay에서 농도의존적으로 항산화능이 증가하는 것을 확인하였다. 각질형성세포에 PM10 300 ㎍/㎖을 단독으로 처리한 군에서는 hyaluronic acid 및 filaggrin이 50% 이상 감소하였으며, 고량강, 유백피, 토복령 추출물을 처리한 군에서는 증가하였다. MMP-1의 경우 PM10 단독처리군에는 55% 이상 증가하였으나, 추출물을 처리한 경우 감소하여 콜라겐, 엘라스틴의 분해를 저해하는 것으로 평가된다. 또한 제브라피쉬 배아를 이용한 ROS 측정의 경우 추출물을 처리하였을 때 감소되는 것을 확인하였다. 특히 토복령 추출물의 25 ㎍/ml에서 음성대조군과 유사한 형광의 세기를 나타내어 ROS의 생성이 유의적으로 감소한 것을 확인하였다. 본 연구를 통하여 선별된 한약재 소재인 고량강, 유백피, 토복령은 미세먼지로부터 피부를 보호하거나 개선할 수 있는 소재로서 피부 개선을 위한 안티에이징 제품으로 활용될 수 있을 것으로 사료된다.

Due to the increase in fine dust caused by environmental pollution, oxidative damage and aging of the skin are accelerated. In this study, the antioxidant, hyaluronic acid, filaggrin, MMP-1, and ROS level of selected herbal extracts were evaluated to confirm the protective efficacy of keratinocytes treated PM10. As a result, the antioxidant capacity of 1,1-diphenyl-2-picrylhydrazyl(DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid(ABTS), and FRAP assay increased in a concentration-dependent manner. Keratinocytes the group treated with 300 ㎍/ml of PM10, hyaluronic acid and filaggrin decreased by more than 50%, and increased in the group treated with extracts of Alpinia officinarum, Ulmus macrocarpa, and Ulmus macrocarpa but decreased when the extract was treated, which is evaluated as inhibiting the degradation of collagen and elastin. In addition, in the case of ROS measurement using zebrafish embryos, it was confirmed that the extract was reduced when the extract was treated 25 ㎍/ml, the intensity of fluorescence similar to the negative control was shown, confirming that the production of ROS was significantly reduced. Through this study, the selected oriental medicinal materials, Alpinia officinarum, Ulmus macrocarpa, and Ulmus macrocarpa, protect the skin from fine dust. It is thought that it can be used as an anti-aging product for skin improvement as a material that can be improved or improved.

키워드

참고문헌

  1. Mukherjee A, "Agrawal M. World air particulate matter: Sources, distribution and health effects", Environ. Chem. Lett, Vol.15, pp. 283-309, (2017). 
  2. Ngoc LTN, Park D, Lee Y, Lee YC, "Systematic Review and Meta-Analysis of Human Skin Diseases Due to Particulate Matter", Int. J. Environ. Res. Public Health, Vol.14, pp. 1458-1469, (2017). 
  3. Kim KE, Cho D, Park HJ, "Air pollution and skin diseases: Adverse effects of airborne particulate matter on various skin diseases" Life Sci. Vol.152 pp. 126-34, (2016). 
  4. Hye Jin Kim, Mi Young Yoo, Hyun Ki Kim, Byung Ho Lee, Kwang-Suk Oh, Ho Won Seo, Gyu Hwan Yon, Odontuya Gendaram, Dae Young Kwon, Young Sup Kim and Shi Yong Ryu, "Vasorelaxation Effect of the Flavonoids from the Rhizome Extract of Alpinia officinarum on Isolated Rat Thoracic Aorta", Kor. J. Pharmacogn, Vol.37, No.1 pp. 55-58, (2006). 
  5. Myoung Lae Cho1, Yu-Na Oh, Jin-Gyeong Ma, Su-Jin Lee, Young-Hee Choi, Dong-Hwa Son, Jang Eun Hee, Jong-Yea Kim, "Effect of extract temperature and duration on antioxidant activity and sensory characteristics of Ulmus pumila bark extract", Korean J. Food Preserv, Vol.23M No.7 pp. 995-1003, (2016). 
  6. Tae-Won Jang, Chang-Gun Oh, Jae-Ho Park, "Antioxidant activity and protective effects on oxidative DNA damage of Smilax china root", J Appl Biol Chem, Vol.61, No.2 pp. 109-117, (2018). 
  7. Shu XS, Gao ZH, Yang, "XL Anti-inflammatory and antinociceptive activities of Smilax china L. aqueous extract", J Ethnopharmacol, Vol.103, pp. 327-332, (2006). 
  8. Cheng DS, Hua XL, "Today's research of Smilax china", J Chin Med Tradit Chin Med, Vol.29, pp. 90-93, (2006). 
  9. Li YL, Gan GP, Zhang HZ, Wu HZ, Li CL, Huang YP, Liu YW, Liu JW, "A flavonoid glycoside isolated from Smilax china L. rhizome invitro anticancer effects on human cancer cell lines", J Ethnopharmacol, Vol.113, pp. 115-124, (2007). 
  10. Myeong-Wook Kim, Eun-Ho Lee, Ye-Jin Kim, Tae-Soon Park, Young-Je Cho, "Beauty food activities of wild-cultivated Ginseng (Panax ginseng C.A. Meyer) ground par",, J Appl Biol Chem, Vol.61, No.1 pp. 33-38, (2018). 
  11. Jae-Bum Jo, Hye-Jin Park, Eun-Ho Lee, Jae-Eun Lee, Su-Bin Lim, Shin-Hyub Hong, Young-Je Cho, "Whitening and anti-wrinkle effect of Pinus koraiensis leaves extracts according to the drying technique" J Appl Biol Chem, Vol.60, No.1 pp. 73-78, (2017). 
  12. Eun-Young Hwang, Dong-Hee Kim, Hui-Jeong Kim, Jo-Young Hwang, Tae-Soon Park, In-Sun Lee, and Jun-Ho Son, "Antioxidant Activities and Nitric Oxide Production of Medicine Plants in Gyeongsangbukdo (Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluriflorum, Paeonia lactiflora)", J. Appl. Biol. Chem, Vol.54, No.3 pp. 171-177, (2011). 
  13. Min-Jung Kim and Eunju Park, "Feature Analysis of Different In Vitro Antioxidant Capacity Assaysand Their Application to Fruit and Vegetable Samples", J Korean Soc Food Sci Nutr, Vol.40, No.7 pp. 1053-1062, (2011). 
  14. Lee SE, Seong NS, and Bang JK, "Antioxidative activities of korean medicineal plants", Korean J Medicinemal Crop Sci Vo.11, pp. 127-134, (2003). 
  15. Benzie IFF and Strain JJ, "The ferric reducing ability of plasma (FRAP) asa measure of "antioxidant power" The FRAP assay", Anal Biochem, Vol.239, pp. 70-76, (1996). 
  16. Jung Im Lee, Jung Hwan Oh, So Young Park, Hye Ran Kim, Kyung Im Jung, Byung-Jin Jeon, "Dongmin Kim, Chang-Suk Kong, UV-induced Photodamage-attenuating Properties of Water Extract from Lentinuls edodes", Journal of Life Science, Vol.30. No.10 pp. 877-885, (2020). 
  17. Kim, G. Y., Lee, S. J., Jeon, M. J., Kim, B. M., Kim, G. T., Kang, S. M., Lee, K. Y., Shin, E. J., Kim, S. Y. and Kim, Y. M, "Anti-wrinkle and skin turnover improvement effects of niacinamide-dipeptide convergence", Asian J. Beauty Cosmetol. Vol.16 pp. 243-254, (2018). 
  18. Kim, J. and Lee, C. M. "Anti-inflammatory effects and influence on fibroblast growth of astaxanthin-cyclodextrin namoparticle",. J. Chitin Chitosan, Vol.23, pp. 170-175, (2018). 
  19. Hak Yin Yu, In Jun Yang, V.R Lincha, In Sik Park, Dong-Ung Lee, Heung Mook Shin, "The Effects of the Fruits of Foeniculum vulgare on Skin Barrier Function and Hyaluronic Acid Production in HaCaT Keratinocytes", Journal of Life Science, Vol.25, No.8 pp. 880-888, (2015). 
  20. Dong-Hee Kim, Tae-Soon Park, Jun-Ho Son, "Anti-inflammatory effects of the Rubus occidentalis seed extracts on UV-B induced inflammation in HaCat cells,", J Appl Biol Chem, Vol.59, No.3 pp. 247-253, (2016). 
  21. Elias PM, Wood LC, Feingold KR, "Epidermal pathogenesis of inflammatory dermatoses", Am J Contact Dermat, Vol.10, pp. 119-126, (1999). 
  22. Fuchs E, "Epidermal differntiation and keratin gene expression". J Cell Sci, Vol.17, pp. 197-208, (1993). 
  23. Steinert PM, Marekov LN, "The poteins elatin, filaggrin, keratin intermediate filaments, loricrin, and small proline-rich proteins 1 and 2 are isodipeptide cross-linked components of the human epidermal cornified cell envelope", J Bio Chem, Vol.270, pp. 17702-17711, (1995). 
  24. Taiieb, "A Hypothesis: from epidermal barrier dysfunction to atopic disorders", Contact Dermatitis, Vol.41, pp. 177-180, (1999). 
  25. Vera Mugoni, Annalisa Camporeale, Massimo M. Santoro, "Analysis of Oxidative Stress in Zebrafish Embryos", J Vis Exp. Vol.89 pp. 513-528. (2014).