Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Sleep deprivation induces structural changes in the adult rat testis: The protective effects of olive oil

  • Fatemeh Karimi (Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences) ;
  • Ali Noorafshan (Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences) ;
  • Saied Karbalay-Doust (Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences) ;
  • Maryam Naseh (Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences)
  • Received : 2022.08.21
  • Accepted : 2022.11.29
  • Published : 2023.03.31
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Abstract

Objective: Sleep deprivation (SD) is a common problem in today's stressful lifestyle and have physiological consequences, including reproductive dysfunction and infertility. As an antioxidant, olive oil may be effective in reducing testicular and spermatological damage by decreasing the production of free radicals. Methods: This study investigated the effects of olive oil on sperm quality and testicular structure using stereological methods to assess rats with SD. Results: When comparing SD group to grid floor+distilled water (GR) group, we found that the sperm count and motility, as well as the percentage of slow progressive sperm was significantly lower in SD group (p<0.05), but the percentage of immotile sperm was higher (p<0.01). However, no improvement was observed in sperm count or motility after concomitant treatment of SD group with olive oil. Stereological examinations revealed no significant change in the total volumes of the seminiferous tubules, interstitial tissue, and germinal epithelium in the study groups. Conversely, the total number of testicular cell types was significantly lower in SD group than in GR group. Although the total number of Sertoli and Leydig cells was significantly higher in the S +olive oil group than in the untreated SD group, no significant difference in the total number of other testicular cell types was observed between the two groups. Conclusion: SD potentially induced structural changes in testis that affected sperm count and motility. However, olive oil only improved the total number of Sertoli and Leydig cells in the animals with SD and did not improve sperm count and motility.

Keywords

Acknowledgement

The authors would like to thank Ms. A. Keivanshekouh at the Research Consultation Center of Shiraz University of Medical Sciences for improving the use of English in the manuscript.

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