Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Novel polymorphisms of dopa decarboxylase gene and their association with lamb quality traits in Indonesian sheep

  • Ratna Sholatia Harahap (Graduate School of Animal Production and Technology, Faculty of Animal Science, IPB University) ;
  • Ronny Rachman Noor (Department of Animal Production and Technology, Faculty of Animal Science, IPB University) ;
  • Yuni Cahya Endrawati (Department of Animal Production and Technology, Faculty of Animal Science, IPB University) ;
  • Huda Shalahudin Darusman (Department of Anatomy Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University) ;
  • Asep Gunawan (Department of Animal Production and Technology, Faculty of Animal Science, IPB University)
  • Received : 2022.06.09
  • Accepted : 2022.11.02
  • Published : 2023.06.01
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Abstract

Objective: This study aimed to investigate the polymorphisms of the dopa decarboxylase (DDC) gene and association analysis with lamb quality and expression quantification of the DDC gene in phenotypically divergent Indonesian sheep. Methods: The totals of 189 rams with an average body weight of 24.12 kg at 10 to 12 months were used to identify DDC gene polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 189 rams, several rams representing various sheep genotypes were used for an association study between genotypes and phenotypic traits with proc general linear model (GLM) analysis. In addition, the gene expression analysis of the DDC mRNA in the phenotypically divergent sheep population was analyzed using quantitative reverse-transcription PCR. Results: The DDC gene (g. 5377439 G>A) showed polymorphisms that indicated three genotypes: AA, AG, and GG. The DDC gene polymorphism was significantly associated (p≤0.05) with carcass characteristics including carcass percentage, carcass length, hot and cold carcass; physical properties of lamb quality including pH value; retail cut carcass; fatty acid composition such as fat content, pentadecanoic acid (C15:0), tricosylic acid (C23:0), lignoceric acid (C24:0), oleic acid (C18:1n9c), elaidic acid (C18:1n9t), nervonic acid (C24:1), linoleic acid (C18:2n6c), arachidonic acid (C20:4n6), cervonic acid (C22:6n3); and mineral content including potassium (K). The GG genotype of the DDC gene had the best association with lamb quality traits. The DDC gene expression analysis mRNA showed no significant difference (p≥0.05) between lamb quality traits. Conclusion: The DDC gene could be used as a potential candidate gene to improve lamb quality.

Keywords

Acknowledgement

This research was funded by the Directorate General of Resources for Science, Technology and Higher Education, Ministry of Research, Technology and Higher Education Contract. Number: 001/E5/PG.02.00PT/2022 date 16 March 2022. The funder had no role in carrying out the experiment, writing the manuscript, and the decision to submit the manuscript for publication.

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