한국환경과학회지 (Journal of Environmental Science International)

  • 제32권4호
  • /
  • Pages.221-232
  • /
  • 2023
  • /
  • 1225-4517(pISSN)
  • /
  • 2287-3503(eISSN)
한국환경과학회 (The Korean Environmental Sciences Society)
권호 표지
DOI QR코드

DOI QR Code

추출 온도 및 용매에 따른 아메리카동애등에(Hermetia illucens) 유충 추출물의 항산화 효과

Antioxidant Effects of Hermetia illucens Larvae Extracts Using Different Extraction Temperatures and Solvents

  • 박지영 (농촌진흥청 국립농업과학원 농업생물부 곤충양잠산업과) ;
  • 구본우 (농촌진흥청 국립농업과학원 농업생물부 곤충양잠산업과) ;
  • 김용순 (농촌진흥청 국립농업과학원 농업생물부 곤충양잠산업과) ;
  • 박관호 (농촌진흥청 국립농업과학원 농업생물부 곤충양잠산업과)
  • Ji Yeong Park (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Bonwoo Koo (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Yong-Soon Kim (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA) ;
  • Kwanho Park (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA)
  • 투고 : 2023.01.25
  • 심사 : 2023.03.15
  • 발행 : 2023.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study investigated the antioxidant effect of Hermetia illucens larvae using different extraction temperatures and solvents. We found significant differences in total phenolic content (TPC), total flavonoid content (TFC), an in three antioxidant indexes 2,2-diphenyl-1-picrylhydrazyl (DPPH), 1,1'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and ferric-reducing antioxidant power (FRAP) contents, among the samples depending on the extraction temperature and solvent used. The sample extracted with water at 45℃ (HIW-45℃) showed the highest TPC, DPPH, ABTS, and FRAP contents, while the sample extracted with water at 90℃ (HIW-90℃) showed the highest TFC. These differences can be due to the different chemical structures of the extracted components. Based on these results, HIW-45℃ was the optimal extraction method for Hermetia illucens. We intend to further investigate the availability of functional materials for Hermetia illucens using this method.

키워드

과제정보

본 연구는 농촌진흥청 연구사업(연구개발과제명: 동애등에를 이용한 과채류 부산물 분해 및 유충 사료화, PJ015960)의 지원에 의해 이루어진 것입니다.

참고문헌

  1. Alvarez, D., Wikinson, K. A., Treihou, M., Tene, N., Castillo, D., Sauvain, M., 2019, Prospecting peptides isolated from black soldier fly (Diptera: stratiomyidae) with antimicrobial activity against Helicobacter pylori (Campylobacterales: Helicobacteraceae), J. Insect Sci., 19, 1-5. https://doi.org/10.1093/jisesa/iez120
  2. Beecher, G. R., 2003, Overview of dietary flavonoids: nomenclature, occurrence and intake, J. Nutr., 133, 3248-3254. https://doi.org/10.1093/jn/133.10.3248S
  3. Benzie, I. F. F., Strain, J. J., 1996, The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay, Anal. Biochem., 239, 70-76. https://doi.org/10.1006/abio.1996.0292
  4. Blois, M. S., 1958, Antioxidant determinations by the use of a stable free radical, Nature, 181, 1199 -1200. https://doi.org/10.1038/1811199a0
  5. Branen, A. L., 1975., Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene, J. Am. Oil Chem. Soc., 52, 59-63. https://doi.org/10.1007/BF02901825
  6. Byun, E. B., Kim, M. J., Kim, S. J., Oh, N. S., Park, S. H., Kim, W. S., Byun, E. H., 2020, Antioxidant activity and neuroprotective effects of ethanol extracts from the core of Diospyros kaki, Korean J. Food Sci. Technol., 52, 60-66.
  7. Cho, H. E., Choi, Y. J., Cho, E. K., 2010., Antioxidant and nitrite scavening activity and α-glucosidase inhibitory effect of water extract from Schizandra chinensis Baillon, J. Korean Soc. Food Sci. Nutr., 39, 481-486. https://doi.org/10.3746/jkfn.2010.39.4.481
  8. Choi, I. H., Park, K. H., Shoi, S. U., Jung, Y. W., Kim, S., Park, C. Y., Chung, T. H., 2022, Effects of dietary Ptecticus tenebrifier on the fecal microbiomes of Bichon fries, J. Environ. Sci. Int., 31, 535-542. https://doi.org/10.5322/JESI.2022.31.6.535
  9. Choi, I. H., Shoi, S. U., Jeong, Y. W., Park, K. H., Kim, T. H., Park, K. W., Chung, T. H., 2022, Effects of dietary Ptecticus tenebrifer powder and canned mixtures on protein digestibility by different breeds of companion dogs, J. Environ. Sci. Int., 31, 285-289. https://doi.org/10.5322/JESI.2022.31.3.285
  10. Choi, J. H., Chung, S. K., 2019, Antioxidant and antimicrobial activities of polyphenols isolated from unripe apples (Malus pumila cv. Hongro), Korean J. Food Preserv., 26, 690-696. https://doi.org/10.11002/kjfp.2019.26.6.690
  11. Choi, Y. H., Yoon, S. Y., Jeon, S. M., Lee, J. Y. Oh, S. M., Lee, S. H., Kim, J. S., 2019, Effects of different levels of Hermetia Illucens on growth performance and nutrient digestibility in weaning pigs, Journal of the Korea Academia-Industrial cooperation Society, 20, 255-261.
  12. Firmansyah, M., Abduh, M. Y., 2019, Production of protein hydrolysate containing antioxidant activity from Hermetia illucens, Heliyon, e02005.
  13. Folin, D., Denis, W., 1912, On phosphotungstic phosphomolybdic compounds as color reagents, J. Biol. Chem.,12, 239-243. https://doi.org/10.1016/S0021-9258(18)88697-5
  14. Gheldof, N., Engeseth, N. J., 2002, Antioxidants capacity of honeys from various flora sources based on the determination of oxygen radical absorbance capacity and inhibition of vitro lipoprotein oxidation in human serum samples, J. Agric. Food Chem., 50, 3050-3055. https://doi.org/10.1021/jf0114637
  15. Hertog, M. G. L., Hollman, P. C. H., van de Putte, B., 1993, Content of potentially anticarcinogenic flavonoids of tea infusions, wines, and fruit juices, J. Agri. Food Chem., 41, 1242-1246. https://doi.org/10.1021/jf00032a015
  16. Jun, H. I., Kim, Y. A., Kim, Y. S., 2014, Antioxidant activities of Rubus coreanus Miquel and Morus alba L. fruits, J. Kor. Soc. Food Sci. Nutr., 43, 381-388. https://doi.org/10.3746/jkfn.2014.43.3.381
  17. Kim, D. H., Moon, Y. S., Son, J. H., 2015, Verification of anti-oxidative activity of Aruncusdioicus, a native plant of Ulleungdo, J. Plant Biol., 42, 55-59. https://doi.org/10.5010/JPB.2015.42.1.55
  18. Kim, D. O., Lee, K. W., Lee, H. J., Lee, C. Y., 2002, Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals, J. Agric. Food Chem., 50, 3713-3717. https://doi.org/10.1021/jf020071c
  19. Kim, J. G., Cho, Y. C., Choi, J. Y., Kim, W. T., Jeong, G. S., Park, K. H., Hwing, S. J., 2008, Ecology of the black soldier fly, Hermetia illucens (diptera: stratmyidae) in Korea. Korean J. Appl. Entomol., 47, 337-343. https://doi.org/10.5656/KSAE.2008.47.4.337
  20. Kim, J. H., Yoon, S. J., Lee, K. H., Kwon, H. J., Chun, S. S., Kim, T. W., Cho, Y. J., 2005, Screening of biological activities of the extracts from basil (Ocimum basilicum L.), Appl. Biol. Chem., 48, 173-177.
  21. Kim, K. C., Kim, J. S., 2020, Effect of varying ethanol concentrations on the extraction properties and physiological activity of Artemisia annua L., Korean J. Food Sci. Technol., 52, 130-137.
  22. Kim, M. W., 2013, Effect of sea buckthorn leaves on hepatic enzyme levels in streptozotocin induced diabetic rats, J. Korean Soc. Food Sci. Nutr., 42, 40-45. https://doi.org/10.3746/jkfn.2013.42.1.040
  23. Kim, S. C., Kwon, H. S., Kim, C. H., Kim, H. S., Lee, C. Y., Cho, S. J., 2016, Comparison of antioxidant activities of pileus and stipe from white beech mushroom (Hypsizygus marmoreus), J. Life Sci., 26, 928-935. https://doi.org/10.5352/JLS.2016.26.8.928
  24. Kim, S. C., Ryu, H. M., Jung, S. M., Lee, Y. H., Kim, H. S., Kim, J. O., Cho, Y. U., Cho, S. J., 2013, Antioxidant and tyrosinase inhibitory activity of Hypsizygus marmorus (brown cultivar) methanol extracts, J. Mushroom Sci. Prod., 11, 254- 260. https://doi.org/10.14480/JM.2013.11.4.254
  25. Kim, S. H., Bae, C. H., Yun, J. H., Sim, J., Han, H. S., 2019, Evaluation of black soldier fly meal as a dietary animal protein source replacing fish meal in Korean catfish Silurus asotus, J. Kor. Soc. Fish Mar. Edu., 31, 1495-1502. https://doi.org/10.13000/JFMSE.2019.12.31.6.1495
  26. Kim, S. K., Choi, Y. R., Park, P. J., Choi, J. H., Moon, S. H., 2000, Purification and characterization of antioxidative peptides from enzymatic hydrolysate of cod teiset protein, Korean J. Fish Aquat. Sci. 33, 198-204.
  27. Kim, T. H., Choi, I. H., Chung, T. H., 2019, Effect of Ptecticus tenebrifer extract on the growth of juvenile Litopenaeus vannamei and water quality, J. Environ. Sci. Int., 28, 1-6. https://doi.org/10.5322/JESI.2019.28.1.1
  28. Kim, Y. E., Yang, J. W., Lee, C. H., Kwon, E. K., 2009, ABTS radical scavenging and anti-tumor effects of Tricholoma matsutake sing. (Pine Mushroom), J. Kor. Soc. Food Sci. Nutr., 38, 555-560. https://doi.org/10.3746/jkfn.2009.38.5.555
  29. Kwon, Y. R., Lee, H. R., Hwang, S. H., Kwon, O. J., Youn, K. S., 2016, Antioxidant activities and physiological properties of Euphorbia humifusa extracts prepared using different solvents, Korean J. Food Preserv., 23, 252-258. https://doi.org/10.11002/kjfp.2016.23.2.252
  30. Lee, J. H., Lee, S. R., 1994, Some physiological activity of phenolic substances in plant foods, Korean J. Food Sci. Technol., 26, 317-323.
  31. Loizzo, M. R., Bonesi, M., Di Lecce, G., Boselli, E., Tundis, R., Pugliese, A., Menichini, F., Frega, N. G., 2013, Phenolics, aroma profile, and in vitro antioxidant activity of Italian dessert passito wine from Saracena (Italy), J. Food Sci., 78, C703-708. https://doi.org/10.1111/1750-3841.12110
  32. MAFRA (Ministry of Agriculture, Food and Rural Affairs), 2021, The investigation of insects industry 2021, Retrieved from, https://www.mafra.go.kr/bbs/mafra/65/330865/artclView. do on Aug 02.
  33. Middleton, Jr. E., Kandaswami, C., 1992, Effects of flavonoids on immune and inflammatory cell functions, Biochem. Pharmacol., 43, 1167-1179. https://doi.org/10.1016/0006-2952(92)90489-6
  34. Park, C. H., 2016, Studies on evaluation of functional feed for animal companions using extract or enzymatic hydrolysate of Tenebrio molitor larvae (Mealworm), M.D. Dissertation, Hanllym University, Gangwon-do, Republic of Korea.
  35. Park, J. Y., Heo, J. C., Woo, S. U., Yun, C. Y., Kang, S. W., Kwang, J. S., Lee, S. H., 2006, Anti-inflammatory and cellular protective effects on hydrogen peroxide-induced cytoxicity of grasshopper extracts., Korean J. Food Preserv., 13, 296-802.
  36. Park, J. Y., Kim, S. Y., Koo, B., Kim, E., Kim, Y. S., Park, K., 2022, Whitening improvement effect of Hermetia illucens larvae extracts., J. Environ. Sci. Int., 31, 883-890. https://doi.org/10.5322/JESI.2022.31.10.883
  37. Park, J. Y., Kwak, K. W., Choi, J. Y., Lee, S. E., Kim, Y. S., Koo, B., Kim, E., Park, K., Kim, S. Y., 2021, Ethanol Extract of Hermetia illucens larvae Inhibits adipogenesis in 3T3-L1 adipocytes, J. Life Sci., 31, 1094-1999.
  38. Park, K. H., Choi, J. Y., Nam, S. H., Kim, S. H., Kwak, K. W., Lee, S. H., Nho, S. K., 2014, Antioxidant activities of black soldier fly, Hermetia illucens, J. Seric. Entomol. Sci., 52, 142-146. https://doi.org/10.7852/jses.2014.52.2.142
  39. Osawa, T., 1994, Novel natural antioxidants for utilization in food and biological systems, In Postharvest Biochemistry of plant Food-Materials in the Tropics, Uritani, I., Garcia, V. V., Mendoza, E. M. Eds., Japan Scientific Societies Press, Tokyo, Japan, 241-251.
  40. Pyo, S. J., Won, J., Kang, D. G., Sohn, H. Y., 2020, Antithrombotic activity of Hermetia illucens (Black soldier fly), J. Life Sci., 30, 386-393.
  41. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., 1999, Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radical Bio Med., 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  42. Singleton, V. L., Orthofer, R., Lamuela-Raventos, R. M., 1999, Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol., 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
  43. Vargas-Abundez, A. J., Randazzo, B., Foddai, M., Sanchini, L., Truzzi, C., Giorgini, E., Olivotto, I., 2019, Insect meal based diets for clownfish: Biometric, histological, spectroscopic, biochemical and molecular implications, Aquaculture, 498, 1-11. https://doi.org/10.1016/j.aquaculture.2018.08.018
  44. Wang, Q., Kuang, H., Su, Y., Sun, Y., Feng, J., Guo, R., Chan, K., 2013, Naturally derived anti-inflammatory compounds from Chinese medicinal plants, J. Ethnopharmacol. 146, 9-39. https://doi.org/10.1016/j.jep.2012.12.013
  45. Yeo, J. S., Chun, S. S., Choi, J. H., 2014, Antioxidant activities of solvent extracts from Rosa multiflora, J. Life Sci., 24, 1217-1223. https://doi.org/10.5352/JLS.2014.24.11.1217
  46. Zhishen, J., Mengcheng, T., Jianming, W., 1999, The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals, Food Chem. 64, 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2