Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Applicability of Next-Generation Sequencing for Analysis of Stomach Contents in Fish

Next-Generation Sequencing을 활용한 어류 위 내용물 분석의 적용 가능성

  • Chae-Jin Park (Department of Integrated Biological Science, Pusan National University) ;
  • Seonbin Yun (Department of Integrated Biological Science, Pusan National University) ;
  • Hyeon-Sik Lee (Department of Integrated Biological Science, Pusan National University) ;
  • Seoyun Jang (Department of Integrated Biological Science, Pusan National University) ;
  • Kang-Hui Kim (Department of Integrated Biological Science, Pusan National University) ;
  • Donghyun Hong (Department of Integrated Biological Science, Pusan National University) ;
  • Gea-Jae Joo (Department of Integrated Biological Science, Pusan National University)
  • Received : 2023.03.22
  • Accepted : 2023.03.30
  • Published : 2023.03.31
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Abstract

The predator-prey interaction in freshwater ecosystems is a crucial area in the ecological study field and one of example to find such interaction is to investigate stomach contents. However, traditional method through visual inspection often induce misidentification, as it depends critically on intactness of physically visible data. In this study, we utilized Next-Generations Sequencing (NGS) technology to test the applicability stomach content analysis and overcome such limitation. NGS was applied to analyze the stomach contents of the Hemibarbus labeo, Tachysurus fulvidraco, and Plecoglossus altivelis collected in the lower part of Nakdong River. As a result, T. fulvidraco had a higher number of Animalia operational taxonomic units (OTUs) intake rate than H. labeo. At the same time, P. altivelis had higher number of Plantae OTUs intake rate than T. fulvidraco and higher Protozoa OTUs intake rate than H. labeo respectively. Therefore, NGS technology application enable to overcome traditional method's limitation and discover hidden interspecific interaction which can further be used in appropriate habitat assessment.

Keywords

Acknowledgement

이 과제는 부산대학교 기본연구지원사업(2년)에 의하여 연구되었음.

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