식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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콩균핵마름병균에 대한 병원성 검정법 확립

Establishment of Pathogenicity Test Method for Macrophomina phaseolina Causing Soybean Charcoal Rot

  • 안소현 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김흥태 (충북대학교 농업생명환경대학 식물의학과)
  • So Hyeon An (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Heung Tae Kim (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
  • Received : 2023.01.12
  • Accepted : 2023.02.10
  • Published : 2023.03.31
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Abstract

콩 균핵마름병을 일으키는 Macrophomina phaseolina의 병원성 평가를 위하여 실험실과 온실 검정법을 확립하였다. 실험실 검정에서는 소립균핵과 균사를 접종원으로 사용하였다. 소립균핵을 접종원으로 사용한 실험실 검정에서 M. phaseolina NSW17-108과 HSM17-034의 발병도는 25℃보다 35℃에서 더 높았다. NSW17-108과 HSM17-034 중에서 참깨에서 분리된 HSM17-034의 발병도가 콩에서 분리된 NSW17-108보다 높았다. M. phaseolina의 균사를 접종원으로 사용한 경우, NSW17-108과 HSM17-034는 35℃에서의 발병도가 접종 5일 만에 80%를 상회하였다. HSM17-034는 25℃에서의 발병도가 접종 5일 후에 80%를 상회하였다. 온실의 병원성 검정에는 소립균핵이 형성된 이쑤시개 또는 potato dextrose agar 배지에서 수확한 소립균핵 자체를 접종원으로 사용하였다. 모든 온실 검정에서 M. phaseolina NSW17-10과 HSM17-034는 접종 방법에 따라 병원균을 접종하고 35-65일 후에 40-60%의 발병도를 보였다. 두 균주 중에서 HSM17-034의 병원성이 NSW17-108보다 강했으며, 이 결과는 실험실 검정 결과와 일치하였다. 본 연구에서 확립한 실험실 및 온실 검정법은 각 방법에 따라 장단점이 있기 때문에, 연구의 목적에 부합할 수 있는 시험법을 선택하여 사용하는 것이 필요하다.

The establishment of a laboratory assay and a greenhouse assay was conducted for evaluating the pathogenicity of Macrophomina phaseolina causing soybean charcoal rot established. In the laboratory assay, microsclerotia and hyphae were used as inoculum. In the laboratory assays using microsclerotia as an inoculum, disease incidences of M. phaseolina NSW17-108 and HSM17-034 were higher at 35℃ than 25℃. Of the two isolates NSW17-108 and HSM17-034, the disease incidence of HSM17-034 isolated from diseased sesame is higher than that of NSW17-108 isolated from diseased soybean. When the mycelia of M. phaseolina were used as an inoculum, the disease incidence of NSW17-108 and HSM17-034 at 35℃ exceeded 80% even after only 5 days of inoculation. Even at 25℃, furthermore, that of HSM17-034 exceeded 80% 5 days later. In the pathogenicity assays at a greenhouse, toothpicks where microsclerotia were produced or microsclerotia harvested from potato dextrose agar medium were used as an inoculum. In all greenhouse assays, M. phaseolina NSW17-108 and HSM17-034 showed 40-60% of disease incidences 35-65 days after inoculation with the pathogen, depending on the inoculation method. Between the two isolates, the pathogenicity of HSM17-034 was stronger than that of NSW17-108, and this result was consistent with laboratory assay results. Since the laboratory and greenhouse test methods tested in this study have different advantages and disadvantages depending on each test method, it is thought that the test method that can meet the purpose of the study should be selected and used.

Keywords

Acknowledgement

This study was carried out with the support of the Rural Development Administration's joint research project (Project No. PJ014956022022).

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