Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Transcriptional Interplay between Malassezia restricta and Staphylococcus Species Co-Existing in the Skin Environment

  • Hyun Oh Yang (Department of Systems Biotechnology, Chung-Ang University) ;
  • Yong-Joon Cho (Department of Molecular Bioscience, Kangwon National University) ;
  • Jae Min Lee (Department of Systems Biotechnology, Chung-Ang University) ;
  • Kyoung-Dong Kim (Department of Systems Biotechnology, Chung-Ang University)
  • Received : 2022.12.13
  • Accepted : 2023.01.02
  • Published : 2023.03.28
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Abstract

Malassezia and Staphylococcus are the most dominant genera in human skin microbiome. To explore the inter-kingdom interactions between the two genera, we examined the transcriptional changes in Malassezia and Staphylococcus species induced upon co-culturing. RNA-seq analyses revealed that genes encoding ribosomal proteins were upregulated, while those encoding aspartyl proteases were downregulated in M. restricta after co-culturing with Staphylococcus species. We identified MRET_3770 as a major secretory aspartyl protease coding gene in M. restricta through pepstatin-A affinity chromatography followed by mass spectrometry and found that the expression of MRET_3770 was significantly repressed upon co-culturing with Staphylococcus species or by incubation in media with reduced pH. Moreover, biofilm formation by Staphylococcus aureus was inhibited in the spent medium of M. restricta, suggesting that biomolecules secreted by M. restricta such as secretory aspartyl proteases may degrade the biofilm structure. We also examined the transcriptional changes in S. aureus co-cultured with M. restricta and found co-cultured S. aureus showed increased expression of genes encoding ribosomal proteins and downregulation of those involved in riboflavin metabolism. These transcriptome data of co-cultured fungal and bacterial species demonstrate a dynamic interplay between the two co-existing genera.

Keywords

Acknowledgement

This work was supported by grants from the National Research Foundation of Korea (NRF) to K.K. (2019R1F1A1061826, 2019R1A4A1024764, and 2022R1A2C1004423).

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