The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Defense Inducer Compounds Up-regulated the Peroxidase, Polyphenol Oxidase, and Total Phenol Activities against Spot Blotch Disease of Wheat

  • Puja Kumari (Department of Plant Pathology, Bihar Agricultural University) ;
  • Chandrashekhar Azad (Department of Plant Pathology, Bihar Agricultural University) ;
  • Ravi Ranjan Kumar (Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University) ;
  • Jyoti Kumari (Department of Plant Pathology, Bihar Agricultural University) ;
  • Kumar Aditya (Department of Plant Pathology, Bihar Agricultural University) ;
  • Amarendra Kumar (Department of Plant Pathology, Bihar Agricultural University)
  • Received : 2022.06.14
  • Accepted : 2022.11.24
  • Published : 2023.04.01
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Abstract

Spot blotch disease of wheat caused by Bipolaris sorokiniana (Sacc.) Shoem is considered as an economically important disease which affects all the growing stages of wheat crop. Therefore, it is important to search some effective management strategies against the spot blotch pathogen. Some synthetic elicitor compounds (salicylic acid, isonicotinic acid, and chitosan) and nano-particles (silver and aluminum) were tested against the pathogen to observe the change in biochemical activity and defense action of wheat plant against spot blotch disease. All the tested elicitor compounds and nano-particles showed a significant increase in activity of peroxidase, polyphenol oxidase (PPO), and total phenol over control. The highest increase in activity of peroxidase was recorded at 72 h from chitosan at 2 mM and 96 h from silver nano-particle at 100 ppm. Maximum PPO and total phenol activity were recorded from chitosan at 2 mM and silver nano-particle at 100 ppm as compared to pathogen-treated and healthy control. The lowest percent disease index, lowest no. of spots/leaf, and no. of infected leaves/plant were found in silver nano-particle at 100 ppm and chitosan at 2 mM, respectively. The use of defense inducer compounds results in significantly up-regulated enzymatic activity and reduced spot blotch disease. Therefore, chitosan and silver nano-particle could be used as alternative methods for the management of spot blotch disease.

Keywords

Acknowledgement

The authors are highly grateful to the Department of Plant Pathology, Bihar Agricultural University, Sabour, Bhagalpur for providing experimental facilities to carry out this work. This research work was financially supported by Directorate of Research, Bihar Agricultural University, Sabour, Bhagalpur.

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