Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Cultivation Characteristics and Optimal Conditions for Mycelial Growth of Volvariella bombycina

흰비단털버섯(Volvariella bombycina)의 배양적 특성 및 균사 배양 조건 설정

  • 민경진 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 박혜성 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 하태문 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 이은지 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과)
  • Received : 2022.11.09
  • Accepted : 2023.03.20
  • Published : 2023.03.31
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Abstract

Volvariella bombycinais an edible mushroom commercially cultivated in Korea and other countries. In this study, the culture characteristics and optimal growth conditions of milky mushroom strains were determined. The growth temperature and pH range of V. bombycinawas extensively investigated between 15 and 35℃ and pH 3-11. For efficient cultivation, 20 types of nutrient sources were selected, specifically, 21 carbon sources, 6 organic nitrogen sources, 7 inorganic nitrogen sources, 13 amino acids, 9 organic acids, and 13 inorganic salts. The impact of each of the selected nutrition sources and their concentration on growth was investigated. The optimum temperature and pH were determined to be 30℃ and pH 8.0, respectively. The optimum concentration of medium elements required for mycelial growth of V. bombycinawas determined to be as follows: carbon source, 0.7% Soluble starch; organic nitrogen source, 0.7% Soytone; inorganic nitrogen source, 1.0% (NH4)2C4H4O6; amino acid, 0.1% cysteine; organic acid, 0.07% lactic acid; and inorganic salt, 0.07 mM CaCl2.

본 연구에서는 V. bombycina 자실체의 안정적인 대량생산에 기반되는 필수적인 균사체 생산을 위한 최적 배양조건을 구명하고자 하였다. V. bombycina 균사체의 효율적 배양을 위한 조건은 최적 배양온도는 30℃, pH 8.0, 탄소원 0.7% Soluble starch, 유기질소원 0.7% Soytone, 무기질소원 1.0% (NH4)2C4H4O6, 아미노산 0.1% Cysteine, 유기산은 0.07% Lactic acid, 그리고 무기염류는 7 mM CaCl2 조건이었다. 새로이 명명한 SSNA V. bombycina의 최적 배지는 추후 V. bombycina의 재배에 널리 활용될 수 있을 것으로 판단된다. 본 연구 결과에 따라 선발된 배양조건으로 흰비단털버섯의 반경을 측정한 결과 37 mm/7days로 24 mm/7days로 측정된 대조군인 PDA 배지보다 균사생육이 빨랐다(Fig. 16, 17). 흰비단털버섯의 기본적인 적정 생육환경을 조사하여 확인된 실험 데이터는 흰비단털버섯 자실체 발생을 위한 흰비단털버섯의 역할을 규명하는데 기여할 것으로 판단된다.

Keywords

Acknowledgement

본 결과물은 농촌진흥청 고유연구사업 원예특작시험연구 '버섯 품목 개발을 위한 유전자원 활용체계 구축(PJ01437002)' 과제의 예산을 지원받아 수행되었습니다.

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