Journal of Applied Biological Chemistry

  • 제66권
  • /
  • Pages.436-446
  • /
  • 2023
  • /
  • 1976-0442(pISSN)
  • /
  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지
DOI QR코드

DOI QR Code

Quantitative analysis of hyperoside and isoquercitrin in methanolic extract of Stewartia koreana leaves using HPLC-DAD

  • Ju-Yeong Kang (Division of Environmental and Forest Science, Gyeongsang National University) ;
  • Yu Hwa Kim (Division of Environmental and Forest Science, Gyeongsang National University) ;
  • Youngdae Yoon (Department of Environmental Health Science, Konkuk University) ;
  • Bong-Gyu Kim (Division of Environmental and Forest Science, Gyeongsang National University)
  • 투고 : 2023.07.24
  • 심사 : 2023.10.20
  • 발행 : 2023.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Since Stewartia koreana leaves are registered with the Food and Drug Administration as edible herbal materials, they are used in the development of functional foods, cosmetics, and medicines. In this study, we established an analysis method that can simultaneously analyze two indicators, hyperoside (quercetin 3-O-galactoside) and isoquercitrin (quercetin 3-O-glucoside) contained in the leaves of S. koreana using HPLC-DAD. In accordance with the Ministry of Food and Drug Safety's health functional food guidelines, the analysis method was verified for specificity, accuracy, precision, limit of quantification, and linearity. The analysis method established in this study showed more than 0.9989 of the correlation coefficient values (r2) for the calibration. The total recovery rates of isoquercitrin and hyperoside were 100.55 and 98.87% with 0.14-0.78 and 0.47-0.67% of the relative standard deviation, respectively. Therefore, it was suggested that the new analytical method would be applied to standardize raw materials and high value-added products originated from the leaves of the S. koreana in the future.

키워드

과제정보

This study was carried out with the support of R&D Program for Forest Science Technology (Project No. "FTIS 2023478B10-2325BC036 ")' provided by Korea Forest Service (Korea Forestry Promotion Institute)

참고문헌

  1. Kwon HJ, Song HK, Kim MY (2008) Taxonomic review of the Stewartia korearia Nakai ex Rehder (Theaceae). Korean J Pl Taxon 38(3): 233-250. doi: 10.11110/kjpt.2008.38.3.233
  2. Yoon YH (2003) A study of woodenware design in the traditional lacquer/lathing technique. Journal of the Korea furniture Society 14(3): 60-69
  3. Ashaolu TJ (2020) Immune boosting functional foods and their mechanisms: A critical evaluation of probiotics and prebiotics. Biomed Pharmacother 130: 110625. doi: 10.1016/j.biopha.2020.110625
  4. Sharifi-Rad M, Berkay Yilmaz Y, Antika G, Salehi B, Tumer TB, Kulandaisamy Venil C, Das G, Patra JK, Karazhan N, Akram M, Iqbal M, Imran M, Sen S, Acharya K, Dey A, Sharifi-Rad J (2021) Phytochemical constituents, biological activities, and health-promoting effects of the genus Origanum. Phytother Res 35(1): 95-121. doi: 10.1002/ptr.6785
  5. Zeng X, Li H, Jiang W, Li Q, Xi Y, Wang X, Li J (2022) Phytochemical compositions, health-promoting properties and food applications of crabapples: A review. Food Chem 386: 132789. doi: 10.1016/j.foodchem.2022.132789
  6. Bae JJ, Kwak JH (2015) Phenolic compounds from the twigs of Stewartia pseudocamellia. Kor J Pharmacogn 46(4): 303-308
  7. Lee TH, Kwak HB, Kim HH, Lee ZH, Chung DK, Baek NI, Kim J (2007) Methanol extracts of Stewartia koreana inhibit cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression by blocking NF-kappaB transactivation in LPS-activated RAW 264.7 cells. Mol Cells 23(3): 398-404
  8. Lee SI, Yang JH, Kim DK (2010) Antioxidant flavonoids from the twigs of Stewartia koreana. Biomolecules & Therapeutics 18(2): 191-196
  9. Ahn D, Lee SI, Yang JH, Kim YH, Lee JH, Park JS, Kim DK (2011) Dihydrochalcone components from the twigs of Stewartia koreana. Kor J Pharmacogn 42(3): 229-232
  10. Roh HJ, Noh HJ, Na CS, Kim CS, Kim KH, Hong CY, Lee KR (2014) Phenolic compounds from the leaves of Stewartia pseudocamellia Maxim. and their Whitening Activities. Biomol Ther (Seoul). 23(3): 283-289. doi: 10.4062/biomolther.2014.140
  11. Lee SS, Bang MH, Park SH, Chung DK, Yang SA (2018) Inhibitory effects of flavonoids isolated from the Leaves of Stewartia koreana on nitric-oxide production in LPS-stimulated RAW 264.7 cells. J Life Sci 28(5): 509-516
  12. Lee TH, Lee GW, Park KH, Mohamed MA, Bang MH, Baek YS, Son Y, Chung DK, Baek NI, Kim J (2014) The stimulatory effects of Stewartia koreana extract on the proliferation and migration of fibroblasts and the wound healing activity of the extract in mice. Int J Mol Med 34(1): 145-152. doi: 10.3892/ijmm.2014.1753
  13. Wang Q, Wei HC, Zhou SJ, Li Y, Zheng TT, Zhou CZ, Wan XH (2022) Hyperoside: A review on its sources, biological activities, and molecular mechanisms. Phytother Res 36(7): 2779-2802. doi: 10.1002/ptr.7478
  14. Kim CH, Kim J-E, Song Y-J (2020) Antiviral activities of quercetin and isoquercitrin against human herpesviruses. Molecules 25(10): 2379. doi: 10.3390/molecules25102379
  15. Taniguchi M, LaRocca CA, Bernat JD, Lindsey JS (2023) Digital database of absorption spectra of diverse flavonoids enables structural comparisons and quantitative evaluations. J Nat Prod 86(4): 1087-1119. doi: 10.1021/acs.jnatprod.2c00720
  16. Kim MH, Jang JH, Oh MH, Heo JH, Lee MW (2014) The comparison of DPPH-scavenging capacity and anti-inflammatory effects of phenolic compounds isolated from the stems of Stewartia koreana Nakai. Nat Prod Res 28(17): 1409-1412. doi: 10.1080/14786419.2014.905560