Journal of Applied Biological Chemistry

  • Volume 66
  • /
  • Pages.144-152
  • /
  • 2023
  • /
  • 1976-0442(pISSN)
  • /
  • 2234-7941(eISSN)
The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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GC/MS and HPLC/PDA characterization of essential oils and phenolic compounds from the aerial parts of common rue (Ruta graveolens)

  • Chang-Dae Lee (Department of Plant Science and Technology, Chung-Ang University) ;
  • Hak-Dong Lee (Department of Plant Science and Technology, Chung-Ang University) ;
  • Yunji Lee (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science) ;
  • Hwan Myung Lee (Division of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University) ;
  • Sanghyun Lee (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2023.03.02
  • Accepted : 2023.04.03
  • Published : 2023.12.31
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Abstract

Two different extraction methods were used to evaluate the medical value of common rue, Ruta graveolens L. (RGL). The results of our 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid assays indicated that the antioxidant activity of RGL essential oil extract obtained through steam distillation was very low, whereas ethanol (EtOH) extracts of RGL showed higher antioxidant activity. RGL essential oil was extracted by steam distillation and characterized by GC/MS analysis. Furthermore, EtOH extracts of RGL were obtained under reflux and analyzed by HPLC/PDA. The GC/MS results indicated that the ketone compounds 2-undecanol acetate, nonyl cyclopropanecarboxylate, and 2-nonanone accounted for more than 70% of the composition of RGL essential oil. The HPLC/PDA analyses indicated that the RGL extracts were rich in phenolic compounds such as protocatechuic acid, rutin, psoralen, xanthotoxin, and bergapten, among which rutin was the most abundant. Collectively, our results demonstrated that RGL contains high levels of phenolic compounds and could thus be commercialized as a valuable plant-derived antioxidant.

Keywords

Acknowledgement

This study was funded by a grant from the Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Eumseong 27709, Republic of Korea.

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