DOI QR코드

DOI QR Code

Effect of Lentinula edodes water extracts and Lentinan on proliferation of myosatellite cell of Bos taurus Hanwoo

표고 자실체 물 추출물과 베타글루칸이 한우 근육위성세포 증식에 미치는 영향

  • Sohee Kim (Department of Forest Science, Chungbuk National University) ;
  • Sehyuk Oh (Department of Animal Science, Chungbuk National University) ;
  • Sanghun Park (Department of Animal Science, Chungbuk National University) ;
  • Eunjin Kim (Department of Forest Therapy, Chungbuk National University) ;
  • Jungseok Choi (Department of Animal Science, Chungbuk National University) ;
  • Hwayong Lee (Department of Forest Science, Chungbuk National University)
  • Received : 2022.11.24
  • Accepted : 2023.02.02
  • Published : 2023.12.31

Abstract

Lentinula edodes is one of the most produced mushrooms in the world. In this study, the effects of L. edodes water extracts and lentinan, a beta-glucan from this mushroom, on the proliferation of Bos taurus Hanwoo myosatellite cells were studied. The betaglucan content of the L. edodes water extract was approximately 15.20% at 85 ℃ for 4 h, 13.64% at 100 ℃ for 4 h, 9.48% at 40 ℃ for 8 h and 8.21% at room temperature for 24 h. L. edodes water extract was added to the culture of Hanwoo myosatellite cells. The expression of the MyoD gene increased in the addition of the extract at 40 ℃ for 8 h and 100 ℃ for 4 h, and the expression of the Myogenin gene increased in the addition of the extract at 40 ℃ for 8 h, but proliferation and activity did not increase compared to no addition. However, the addition of lentinan to the culture of Hanwoo myosatellite cells increased the expression of Myogenin gene related to muscle formation increased and the proliferation and viability of the cells. This study proved that the components of L. edodes can affect the proliferation of Hanwoo myosatellite cells, and further research will help develop the mushroom industry and cultured meat industry in the future.

표고는 전세계적으로 많이 생산되는 버섯 중 하나이다. 베타글루칸은 근아세포의 증식과 분화를 증가시키며, 골격근의 기능을 향상시키는 것으로 알려져 있다. 본 연구에서는 표고 물 추출물과 표고의 베타글루칸인 렌티난이 한우 근육세포의 증식에 미치는 영향을 알아보고자 하였다. 표고 물 추출물의 베타글루칸의 함량은 85 ℃ 4시간 추출조건에서 약 15.20%, 100 ℃ 4시간 추출조건에서 약 13.64%, 40 ℃ 8시간 추출조건와 상온 24시간 추출조건에서 각각 약 9.48%와 약 8.21%였다. 표고 물 추출물을 한우 근육위성세포의 배양에 첨가하였을 때, MyoD 유전자는 40 ℃ 8시간, 그리고 100 ℃ 4시간 추출물에서 그리고 Myogenin 유전자는 40 ℃ 8시간 추출물에서 발현이 증가하였고, 증식과 활성은 무처리에 비하여 증가하지 않았다. 하지만, 근육위성세포의 배양에 렌티난의 첨가는 한우 근육위성세포의 세포분화와 근육형성에 관련된 Myogenin 유전자의 발현, 세포의 증식과 활성을 증가시켰다. 본 연구는 표고의 성분이 한우근육위성세포의 증식에 영향을 줄 수 있는 것을 확인하였으며, 추가연구를 통하여 차후 버섯 산업 및 배양육 산업의 발전에 도움을 줄 수 있을 것이다.

Keywords

Acknowledgement

이 논문은 충북대학교 국립대학육성사업(2020)지원을 받아 작성되었음.

References

  1. Mattila P, lampi R, Ronkainen R, Toivo J, Piironen V (2002) Basic composition and amino acid contents of mushrooms cultivated in Finland. J Agric Food Chem 50: 6419-6422. doi: 10.1021/jf020608m
  2. Rop O, Mlcek J, Jurikova T (2009) Beta-glucans in higher fungi and their health effects. Nutr Rev 67: 624-631. doi: 10.1111/j.1753-4887.2009.00230.x
  3. Royse DJ, Baars J, Tan Q (2017) Current overview of mushroom production in the world. In: Diego CZ, Pardo-Gimenez A (ed) Edible and medicinal mushrooms: technology and applications. John Wiley & Sons Ltd: Hoboken. pp 5-13
  4. Seok SJ, Kim JH (2019) Studies on the biological activity of water extract and solvent fractions of wild Grifola frondosa. J Mushroom 17: 241-246. doi: 10.14480/JM.2019.17.4.241
  5. Jang YA, Kim HN, Yang JC, Lee JW, Kim BA, Lee JT (2015) A study on the possibility of extracts from Sparassis crispa for cosmetic ingredients. J of Korean Oil Chemists' Soc 32: 731-739. doi: 10.12925/jkocs.2015.32.4.731
  6. Kim AR, Kim JE, Park SN (2011) Antioxidative activity and component analysis of Phellinus linteus Extracts. J Soc Cosmet Scientists Korea 37: 309-318. doi: 10.15230/SCSK.2011.37.4.309
  7. Cho JH, Lee JY, Lee MJ, Oh HN, Kang DH, Jhune CS (2013) Comparative analysis of useful β-glucan and polyphenol in the fruiting bodies of Ganoderma spp. J Mushroom Sci Prod 11: 164-170. doi: 10.14480/JM.2013.11.3.164
  8. Lee JS, Lee SH, Jang YM, Lee JD, Lee BH, Jung JY (2011) Macrophage and anticancer activities of feed additives on β-glucan from Schizophyllum commune in breast cancer cells. J Korean Soc Food Sci Nutr 40: 949-955. doi: 10.3746/jkfn.2011.40.7.949
  9. Hong KH, Kim HS, Jang KH, Kang SA (2015) The Improvement Effects of β-glucan on adiposity and serum lipids levels in high fat diet-Induced obese rats. Journal of the Korea Academia-Industrial cooperation Society 16: 3973-3981. doi: 10.5762/KAIS.2015.16.6.3973
  10. Tian J, Ma J, Ma K, Guo H, Baidoo SE, Zhang Y, Yan J, Lu L, Xu H, Wang S (2013) β-glucan enhances antitumor immune responses by regulating differentiation and function of monocytic myeloid-derived suppressor cells. Eur J Immunol 43: 1220-1230. doi: 10.1002/eji.201242841
  11. Li Y, Fan Y, Pan H, Qian H, Qi X, Wu G, Zhang H, Xu M, Rao Z, Wang L, Ying H (2018) Effects of functional β-glucan on proliferation, differentiation, metabolism and its anti-fibrosis properties in muscle cells. Int J Biol Macromol 117: 287-293. doi: 10.1016/j.ijbiomac.2018.05.188
  12. Lee H, Choi S, Lee B, Kim E, Lee W, Han H, Kim K (2020) Effect of Pu'er tea extract on C2C12 myoblast differentiation. Jou rnal of Digital Convergence 18: 585-594. doi: 10.14400/JDC.2020.18.12.585
  13. Heo JW, Song EJ, Kim MJ, Kim JH, Kim SE (2022) Effects of Camellia japonica roots extract on myoblast differentiation and muscle atrophy in association with mitochondrial function. Current Developments in Nutrition 6: 24. doi: 10.1093/cdn/nzac047.024
  14. Joglekar D, Warren R, Browe D, Ekwueme E, Dariani M, Padliya ND, Freeman JW (2020) Investigating the effects of fertilized egg yolk extract on myoblast proliferation and differentiation. Regen Eng Transl Med 6: 125-137. doi: 10.1007/s40883-019-00137-y
  15. Bhat ZF, Bhat HF, Pathak V (2014) Chapter 79 - Prospects for In Vitro Cultured Meat - A Future Harvest. In: Lanza R, Langer R, Vacanti J (ed). Principles of Tissue Engineering, 4th edn. Academic Press, Cambridge, pp 1663-1683
  16. Mourkioti F, Rosenthal N (2005) IGF-1, inflammation and stem cells: interactions during muscle regeneration. Trends Immunol 26: 535-542. doi: 10.1016/j.it.2005.08.002
  17. Moritz MSM, Verbruggen SEL, Post MJ (2015) Alternatives for largescale production of cultured beef: A review. J Integr Agric 14: 208-216. doi: 10.1016/S2095-3119(14)60889-3
  18. Benjaminson MA, Gilchriest JA, Lorenz M (2002) In vitro edible muscle protein production system (mpps): stage 1, fish. Acta Astronaut 51: 879-889. doi: 10.1016/s0094-5765(02)00033-4
  19. Okamoto Y, Haraguchi Y, Yoshida A, Takahashi H, Yamanaka K, Sawamura N, Asahi T, Shimizu T (2022) Proliferation and differentiation of primary bovine myoblasts u sing Chlorella vu lgaris extract for sustainable production of cultured meat. Biotechnol Prog 38: e3239. doi: 10.1002/btpr.3239
  20. Kim KH, Ka KH, Kang JH, Kim S, Lee JW, Jeon BK, Yun JK, Park SR, Lee HJ (2015) Identification of single nucleotide polymorphism markers in the laccase gene of shiitake mushrooms (Lentinula edodes). Mycobiology 43: 75-80. doi: 10.5941/MYCO.2015.43.1.75
  21. Bisen PS, Baghel RK, Sanodiya BS, Thakur GS, Prasad GB (2010) Lentinus edodes: a macrofungus with pharmacological activities. Curr Med Chem 17: 2419-2430. doi: 10.2174/092986710791698495
  22. Ng ML, Yap AT (2002) Inhibition of human colon carcinoma development by lentinan from shiitake mushrooms (Lentinus edodes). J Altern Complement Med 8: 581-589. doi: 10.1089/107555302320825093
  23. Seo S, Jang Y, Ryoo R, Ka KH (2018) Antioxidant properties of water extracts from Lentinula edodes cultivars grown on oak log. Kor J Mycol 46: 51-57. doi: 10.4489/KJM.20180007
  24. Jung KI, Choi YJ, Oh JH, Lee JI, Park SY, Kim HR, Jeon BJ, Kim D, KongCS (2019) Quality characteristics of cookies added with Lentinus edodes water extract. J Life Sci 29: 955-963. doi: 10.5352/JLS.2019.29.9.955
  25. Oh JH, Lee JI, Karadeniz F, Kim HR, Park SY, Jung KI, Jeon BJ, Kim D, Park JH, Kong CS (2021) Quality characteristics of grilled fish paste formulation added with hot water extract powder from Lentinus edodes using One-Way ANOVA. J Korean Soc Food Sci Nutr 50: 307-314. doi: 10.3746/jkfn.2021.50.3.307
  26. Choi MY, Jung TY, Hahm KJ (1995) Cytotoxic effects of hot water soluble polysaccharides from mushroom, Lentinus edodes and vitamin A & E supplementation against P388 cells. korean J Nutrient 28: 1091-1099.
  27. Hwang YJ, Nam NH, Chang MJ, Noh GW, Kim SH (2003) Effect of Lentinus edodes and Pleurotus eryngii extracts on proliferation and apoptosis in human colon cancer cell lines. J Korean Sco Food Sci nutr 32: 217-222. doi: 10.3746/jkfn.2003.32.2.217
  28. de las Heras-Saldana S, Chung KY, Lee SH, Gondro C (2019) Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus. BMC Genomics 20: 156. doi: 10.1186/s12864-019-5530-7
  29. Lee SH, Jang GY, Kim KJ, Lee MJ, Kim TJ, Lee J, Jeong HS (2012) Effect of temperature, solvent concentration, and pH on the β-glucan extraction. Korean J Food & Nutr 25: 871-877. doi: 10.9799/ksfan.2012.25.4.871
  30. Benito-Roman O, Alonso E, Cocero MJ, Goto M (2016) β-glucan recovery from Ganoderma lucidum by means of pressurized hot water and supercritical CO2. Food Bioprod Process 98: 21-28. doi: 10.1016/j.fbp.2015.12.007
  31. Smiderle FR, Morales D, Gil-Ramirez A, de Jesus LI, Gilbert-Lopez B, Iacomini M, Soler-Rivas C (2017) Evaluation of microwave-assisted and pressurized liquid extractions to obtain β-d-glucans from mushrooms. Carbohydr Polym 156: 165-174. doi: 10.1016/j.carbpol.2016.09.029
  32. Kim HJ, Kim HJ (2015) Physicochemical characteristics of Jeju barley and extracted β-glucan. Food Eng Prog 19: 132-138. doi: 10.13050/foodengprog.2015.19.2.132
  33. Braun T, Gautel M (2011) Transcriptional mechanisms regulating skeletal muscle differentiation, growth and homeostasis. Nat Rev Mol Cell Biol 12: 349-361. doi: 10.1038/nrm3118
  34. Luo W, Li E, Nie Q, Zhang X (2015) Myomaker, Regulated by MYOD, MYOG and miR-140-3p, promotes chicken myoblast fusion. Int J Mol Sci 16: 26186-26201. doi: 10.3390/ijms161125946
  35. Mu rphy EJ, Masterson C, Rezoagli E, O'Toole D, Major I, Stack GD, Lynch M, Laffey JG, Rowan NJ (2020) β-Glucan extracts from the same edible shiitake mushroom Lentinus edodes produce differential in-vitro immunomodulatory and pulmonary cytoprotective effects - Implications for coronavirus disease (COVID-19) immunotherapies. Sci Total Environ 732: 139330. doi: 10.1016/j.scitotenv.2020.139330
  36. Choi Y, Lee SM, Chun J, Lee HB, Lee J (2006) Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chemistry 99: 381-387. doi: 10.1016/j.foodchem.2005.08.004
  37. Hwang AY, Yang SC, Kim J, Lim T, Cho H, Hwang KT (2019) Effects of non-traditional extraction methods on extracting bioactive compounds from chaga mu shroom (Inonotus obliquus) compared with hot water extraction. LWT 110: 80-84. doi: 10.1016/j.lwt.2019.04.073
  38. Rosello-Soto E, Parniakov O, Deng Q, Patras A, Koubaa M, Grimi N, Boussetta N, Tiwari BK, Vorobiev E, Lebovka N, Barba FJ (2016) Application of Non-conventional Extraction Methods: Toward a Sustainable and Green Production of Valuable Compounds from Mushrooms. Food Eng Rev 8: 214-234. doi: 10.1007/s12393-015-9131-1
  39. Lau BF, Adbullah N, Aminudin N, Lee HB (2013) Chemical composition and cellular toxicity of ethnobotanical-based hot and cold aqueous preparations of the tiger's milk mushroom (Lignosus rhinocerotis). J Ethnopharmacol 150: 252-262. doi: 10.1016/j.jep.2013.08.034
  40. Sairi AMM, Ismail SI, Sukor A, Rashid NMN, Saad N, Jamian S, Abdullah (2020) Cytotoxicity and anticancer activity of Donkioporiella mellea on MRC5 (Normal human lung) and A549 (Human lung carcinoma) cells lines. Evid Based Complement Alternat Med 2020: 7415672. doi: 10.1155/2020/7415672
  41. Faralli H, Dilworth FJ (2012) Turning on myogenin in muscle: a paradigm for understanding mechanisms of tissue-specific gene expression. Comp Funct Genomics 2012: 836374. doi: 10.1155/2012/836374