The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Anti-inflammatory Effects of Goihwa-san Water Extract via NF-κB Inhibition

괴화산(槐花散)의 NF-κB 기전을 통한 항염증 효과 연구

  • Hyun Hee, Cho (Ga-won Korean Medicine Clinic) ;
  • Ji Young, Choi (Dep. of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, Daegu Hanny University) ;
  • Min, Hwangbo (Dep. of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, Daegu Hanny University) ;
  • Seon Young, Jee (Dep. of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, Daegu Hanny University)
  • 조현희 (가원한의원) ;
  • 최지영 (대구한의대 부속 대구한방병원 안이비인후피부과) ;
  • 황보민 (대구한의대 부속 대구한방병원 안이비인후피부과) ;
  • 지선영 (대구한의대 부속 대구한방병원 안이비인후피부과)
  • Received : 2023.01.16
  • Accepted : 2023.02.11
  • Published : 2023.02.25
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Abstract

Objectives : The purpose of this study was to investigate the anti-inflammatory effect of Goihwa-san water extract(GHS) in vitro & in vivo. Methods : In vitro, we evaluated the anti-inflammatory effect of GHS by comparing the Raw 264.7 cells with 10, 30, 100, 300㎍/㎖ of GHS for 1 hour before Lipopolysaccharide(LPS) to the single LPS treated group. We examined the relative cell viability by MTT assay and the relative level of LPS, Loxoribine(LOX), Peptidoglycan(PGN), Flagellin(FLA)-induced NO production by using Griess reagent and measured relative iNOS protein level and COX-2 protein level by using western blot and Image analyzing system. We measured the production of TNF-α, IL-1β, and IL-6 by each ELISA kits and then measured the relative levels of IκBα, p-IκBα in whole-cell lysate fraction and NF-κB in nuclear fraction by using western blot and Image analyzing system. In vivo, we induced the paw edema by subcutaneous injection of 100㎕/rat CA and measured the swelling volume of paw by using a plethysmometer and then measured the relative iNOS protein level by using western blot. Results : As a result, in vitro, LPS, PGN-induced NO production was significantly inhibited by pretreatment with GHS. GHS reduced LPS, PGN-induced iNOS expression, PGN-induced COX-2 expression and LPS-induced production of cytokine(TNF-α, IL-1β, IL-6). Expression of IκBα was increased by pretreatment with GHS 100㎍/㎖. And the expression of p-IκBα and NF-κB were decreased by pretreatment with GHS 100㎍/㎖. In vivo, CA-induced inflammation rat model was used for the evaluation of the anti-inflammatory effect of GHS. 0.3 or 1.0g/kg of GHS significantly reduced the increases of paw swelling and iNOS expression in paw tissues. Conclusions : These results show that GHS can decrease inflammatory response via inhibition of the NF-κB pathway in vitro. And in vivo, the anti-inflammatory effect suggest the clinical basis of GHS for the treatment of inflammatory diseases.

Keywords

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