The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Anti-inflammatory Effects of Tanghwajitong-san through Inhibition of NF-κB and MAPK

탕화지통산(湯火止痛散)의 NF-κB 및 MAPK 억제를 통한 항염증 효과

  • Min Jung, Ko (Woobo Korean Medical Clinic Changwon Branch) ;
  • Seon Young, Jee (Dep. of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, Daegu Hanny University) ;
  • Min, Hwangbo (Dep. of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, Daegu Hanny University)
  • 고민정 (우보한의원 창원점) ;
  • 지선영 (대구한의대학교 한의과대학 안이비인후피부과학교실) ;
  • 황보민 (대구한의대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2023.01.16
  • Accepted : 2023.02.07
  • Published : 2023.02.25
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Abstract

Objectives : The purpose of this study was to evaluate the anti-inflammatory effect of Tanghwajitongsan(THJTS) through inhibition of NF-κB and MAPK. Methods : We evaluated cell survival rate by MTT assay, NO production by nitrite content in the culture medium. We quantified TNF-α, IL-1β, IL-6 and PGE2 of the cultured supernatant by ELISA. And we evaluated the effect of THJTS on protein expression of NF-κB, MAPK, iNOS and COX-2 by Western blot analysis. THJTS ameliorates LPS-activated alterations in protein expression of NF-κB, p-38, iNOS and COX-2 and production of NO, pro-inflammatory cytokines and PGE2. Also, THJTS ameliorates LOX, PGN and FLA-activated alterations in protein expression of NO, iNOS. THJTS ameliorates only PGN-activated alterations in protein expression of COX-2. Results : THJTS ameliorates LPS-activated alterations in protein expression of NF-κB, p-38, iNOS and COX-2 and production of NO, pro-inflammatory cytokines and PGE2. Also, THJTS ameliorates LOX, PGN and FLA-activated alterations in protein expression of NO, iNOS. THJTS ameliorates only PGN-activated alterations in protein expression of COX-2. Conclusions : This study can provide scientific evidence for the anti-inflammatory effects and underlying mechanisms of THJTS.

Keywords

References

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