한국식품저장유통학회지 (Food Science and Preservation)

  • 제30권1호
  • /
  • Pages.65-77
  • /
  • 2023
  • /
  • 3022-5477(pISSN)
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  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
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Immunomodulatory effects of six Acetobacter pasteurianus strains in RAW-Blue macrophage

  • Sun Hee Kim (Fermented and Processed Food Science Division, Department of Agrofood Resource, NIAS, RDA) ;
  • Woo Soo Jeong (Fermented and Processed Food Science Division, Department of Agrofood Resource, NIAS, RDA) ;
  • So-Young Kim (Fermented and Processed Food Science Division, Department of Agrofood Resource, NIAS, RDA) ;
  • Soo-Hwan Yeo (Fermented and Processed Food Science Division, Department of Agrofood Resource, NIAS, RDA)
  • 투고 : 2022.10.24
  • 심사 : 2022.12.02
  • 발행 : 2023.02.28
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초록

In this study, we investigated the immunological properties of six strains of Acetobacter pasteurianus through nuclear factor-kappa B/activator protein-1 (NF-κB/AP-1) transcription factor activation and nitric oxide (NO) and cytokine production in macrophages. We found that the six A. pasteurianus strains had no significant inhibitory effect on the cell viability of RAW-BlueTM cells at the concentration of (25, 50, 100 CFU/macrophage). The production of NO and cytokines (TNF-α, IL-1β, and IL-6) showed different abilities of immune activation for each strain, and it was 0.7 to 0.9 times higher than that of the LPS (100 ng/mL, v/v) positive control and 7 to 8 times superior to that of the negative control group. To explore the underlying mechanism, we evaluated the mRNA expression of pro-inflammatory genes. Consequently, we found that inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expression including genes expression of cytokines were elevated by the six A. pasteurianus treatment. These results suggested that the six strains of A. pasteurianus have an excellent industrial application value as a functional material for the purpose of enhancing immune function.

키워드

과제정보

The project was supported by the Research Program for Agricultural Science & Technology Development (Project No. PJ014161032022), the National Institute of Agricultural Sciences, Rural Development Administration, Korea.

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