Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Biological Control of White Rot in Apple Using Bacillus spp.

Bacillus spp.를 이용한 사과 겹무늬썩음병의 생물학적 방제

  • Ha-Kyoung Lee (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Jong-Hwan Shin (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Seong-Chan Lee (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • You-Kyoung Han (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 이하경 (국립원예특작과학원 원예특작환경과) ;
  • 신종환 (국립원예특작과학원 원예특작환경과) ;
  • 이성찬 (국립원예특작과학원 원예특작환경과) ;
  • 한유경 (국립원예특작과학원 원예특작환경과)
  • Received : 2023.11.23
  • Accepted : 2023.12.06
  • Published : 2023.12.31
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Abstract

Apple white rot, caused by Botryosphaeria dothidea, is one of the important diseases in Korea. B. dothidea can cause pre- and postharvest decay on apple fruit as well as canker and dieback of apple trees. In this study, we isolated bacteria from the trunk of apple trees and tested their antagonistic activity against B. dothidea. Five bacterial isolates (23-168, 23-169, 23-170, 23-172, and 23-173) were selected that were most effective at inhibiting the mycelial growth of the pathogens. The isolate 23-172 was identified as Bacillus amyloliquefaciens and four isolates 23-168, 23-169, 23-170, and 23-173 were identified as Bacillus velezensis by RNA polymerase beta subunit (rpoB) and DNA gyraseA subunit (gyrA) gene sequencing. All isolates showed strong antagonistic activity against B. dothidiea as well as Colletotrichum fructicola and Diaporthe eres. All isolates exhibited cellulolytic, proteolytic and phosphate solubilizing activities. In particular, two isolates 23-168, 23-169 were shown to significantly reduce the size of white rot lesions in pretreated apple fruits. These results will provide the basis for the development of a fungicide alternative for the control of white rot of apple.

본 연구에서는 사과 겹무늬썩음병을 유발하는 Botryosphaeria dothidea를 억제하는 길항미생물을 선발하였고, 선발한 길항미생물의 항균 활성과 길항 효소 생성 여부를 확인하였다. 선발된 길항미생물 중 4종은 Bacillus velezensis로 동정되었고, 1종은 B. amyloliquefaciens로 동정되었으며 이 길항미생물은 모두 겹무늬썩음병균에 강한 항균 활성을 가지고 있었다. 또한 선발된 5종의 길항미생물은 탄저병균인 Colletotrichum fructicola, 줄기마름병인 Diaporthe eres도 효과적으로 균사생장을 억제하였다. 선발된 길항미생물을 대상으로 cellulase, protease, phosphate solubilization 효소 활성을 분석한 결과 모두 효소를 분비하여 병원균의 생장을 억제 및 사멸시키고 식물 생장촉진 활성을 가지는 것으로 확인되었다. 사과 과실에서 겹무늬썩음병균과 선발된 길항미생물을 동시 접종하여 발병 억제 효과를 검정한 결과 B. velezensis 23-168 균주가 가장 효과적으로 발병을 억제하였다. 따라서 선발된 5종의 길항미생물은 사과 진균 방제를 위한 기초자료로 사용할 수 있을 것으로 생각된다.

Keywords

Acknowledgement

This work was carried out with the support of "Development of efficient detection and diagnosis technique for apple and peach latent pathogens in response to climate changes (Project No. RS-2020-RD009293)" Rural Development Administration, Republic of Korea.

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