대한한의학방제학회지 (Herbal Formula Science)

  • 제31권4호
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  • Pages.315-325
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  • 2023
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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RBL-2H3 세포에서 당귀(當歸) 및 산수유(山茱萸) 복합추출물의 알레르기 개선에 대한 효과

Anti-Allergic Effects of Angelica gigas Nakai and Corni fructus extract (AC) on degranulation and production of cytokine in RBL-2H3 mast Cells

  • 오태우 (한국한의학연구원 한의기술응용센터)
  • Tae Woo Oh (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM))
  • 투고 : 2023.11.03
  • 심사 : 2023.11.13
  • 발행 : 2023.11.30
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초록

Objectives : Recently, research has been actively conducted on the efficacy of complexes based on oriental medicine prescriptions for improving immune activity and allergies. In this study, In this study, we aimed to examine the effect of Angelica gigas Nakai and Corni fructus extract (AC), medicinal herbs, among candidate drugs derived through preliminary experiments with various components of oriental medicine prescriptions for allergies, on allergies in RBL-2H3 cells. Methods : We evaluated the effect of the ethanol extract of Ulmus on the allergic inflammatory response in anti-DNP-IgE activated DNP-HSA in RBL-2H3 cells. Cell toxicity was determined by WST-1 assay and the markers of degranulation such as beta-hexosaminidase, histamine, TNF-α and IL-6 production of inflammatory mediators and FcεRI-mediated expression. Results : The results showed that treatment with AC extract (20, 40 and 80㎍/㎖) noncytotoxic levels and significantly inhibited the release of β-hexosaminidase, histamine and the production of TNF-α and IL-6 in RBL-2H3 by the antigen stimulation. Conclusions : These results indicate that AC extract exhibits anti-allergic activity through inhibition of degranulation and inhibition of inflammatory mediators and cytokine release. These findings suggest that AC extract may have potential as a prophylactic and therapeutic agent for the treatment of various allergic diseases.

키워드

과제정보

본 연구는 한국한의학연구원 주요사업을 통해 연구비의 지원을 받아 수행되었습니다. (과제고유번호: KSN1823231) 이에 감사드립니다.

참고문헌

  1. Amin K. The role of mast cells in allergic inflammation. Respiratory Medicine. 2012;106(1):9-14.  https://doi.org/10.1016/j.rmed.2011.09.007
  2. Marquardt DL, Walker LL. Dependence of mast cell IgE-mediated cytokine production on nuclear factor-κB activity. Journal of Allergy and Clinical Immunology. 2000;105(3):500-505.  https://doi.org/10.1067/mai.2000.104942
  3. Galli SJ. New concepts about the mast cell. N Engl J Med. 1993;328(4):257-265.  https://doi.org/10.1056/NEJM199301283280408
  4. KM Ahn. Role of mast cell in allergic inflammation and innate immunity. Korean Journal of Pediatrics. 2004;47:11-15. 
  5. Kim SA, Oh HK, Kim JY, Hong JW, Cho SI. A Review of Pharmacological Effects of Angelica gigas, Angelica sinensis, Angelica acutiloba and their Bioactive Compounds. J Korean Oriental Med. 2011;32(4):1-24. 
  6. Professors of herbology in colleges of oriental medicine. Herbology. Yeonglimsa:Seoul. 2004:632-634. 
  7. Kim SA, Oh HK, Kim JY, Hong JW, Cho SI. A Review of Pharmacological Effects of Angelica gigas, Angelica sinensis, Angelica acutiloba and their Bioactive Compounds. J Korean Oriental Med. 2011;32(4):1-24. 
  8. Lee YG, Kim JK. The study of the oriental medicine extract on the hair growth effect : I. The effect of the mixture extract of Polygoni multiflori Radix, Angelicae gigantis Radix and Lycii Fructus on the hair growth. Kor. J. Herbology. 2004;19(2):83-90. 
  9. Kang SY, Kim YC. Decursinol and decursin protect primary cultured rat cortical cells from glutamate-induced neurotoxicity. J Pharm Pharmacol. 2007;59(6):863-870.  https://doi.org/10.1211/jpp.59.6.0013
  10. Kang SY, Lee KY, Sung SH, Kim YC. Four new neuroprotective dihydropyranocoumarins from Angelica gigas. J Nat Prod. 2005;68(1):56-59.  https://doi.org/10.1021/np049705v
  11. Jeon YI, Park CS, Park CG. An Experimental Study of Effect on Brain Damage and Neuroprotective Effect of Angelicae Gigantis Radix extract against Cerebral Ischemia in Rats. Kor. J. Herbology 2003;18(4):25-35. 
  12. Chung SR, Jeune KH, Park SY, Jang SJ. Toxicity and lectins constituents from the seed of Cornus officinalis. Kor. J. Pharmacogn. 1993;24:177-182. 
  13. Park YK, Whang WK and Kim HI. The antidiabetic effects of extracts from Cornus officinalis seed. Chung-Ang J. Pharm. Sci. 1995;9:5-11. 
  14. Kim BR, Lee JS, Kim DY, Kim BK, Lee HS, Lee SY, Kwon HJ. Antioxidant and Antibacterial Activities of the bark of Fraxinus Rhynchophylla Hance. Kor. J. Aesthet. Cosmetol. 2015;13:339-344. 
  15. Seong JM, Park NY and Lee SH. Effect of Shizandra chinensis and pine needle on growth of pathogenes relate to acne. Kor. J. Microbiol. 2003;31:69-74. 
  16. Jeon YH, Park MH, Kim MR. Antibacterial Activity of the Ethanol Extract from Cornus officinalis against Some Bacteria Related to Foodborn Illness and Food Spoilage. J. East. Asian. Soc. Dietary Life. 2012;22:692-700. 
  17. Seo YB, Kil GJ, Lee YK, Lee YC. Study on the Corni Fructus about the ANti-allergic Action, Kor, J, Heroblogy. 2002:17(1);1-12. 
  18. Busse WWW, Lemanske RF Jr. Asthma. N Engl J. Med. 2001;44(5):350-62.  https://doi.org/10.1056/NEJM200102013440507
  19. Moon SH, Jang HJ, Park YS, Lee WY, Lim DH, Kim JH. Fractional exhaled nitric oxide in Korean children with allergic rhinitis. Allergy Asthma Respir Dis. 2015;3(6):439-445.  https://doi.org/10.4168/aard.2015.3.6.439
  20. Woo HS, Kim CH. The Review of Oriental Medical Therapy on Allergic Rhinitis. J Korean Oriental Med.2006;27(1):155-64. 
  21. DO HJ, TW Oh. Effect of Ullmus macrocarpa Hance Ethanol extract (Ulmus) on Improvement of allergic responses in RBL-2H3 mast Cells. Herbal Formula Science. 2021;29(4):191-203.  https://doi.org/10.14374/HFS.2021.29.4.191
  22. Lee E, Chol EJ, Cheong H, Kim YR, Ryu SY, Kim KM. Anti-allergic actions of the leaves of castanea crenata and isolation of an active component responsible for the inhibition of mast cell degranulation. Archives of Pharmacal Research. 1999;22:320-323.  https://doi.org/10.1007/BF02976372
  23. Kay AB. Allergy and allergic diseases. First of two parts. N Engl J Med 2001; 344(1): 30-37.  https://doi.org/10.1056/NEJM200101043440106
  24. Gurish MF, Austen KF. The diverse roles of mast cells. J Exp Med 2001; 194(1): F1-5.  https://doi.org/10.1084/jem.194.1.F1
  25. Boyce, J.A. Mast cells and eicosanoid mediators:a system of reciprocal paracrine and autocrine regulation. Immunol Rev. 2007;217(1):168-185.  https://doi.org/10.1111/j.1600-065X.2007.00512.x
  26. Mekori YA, Metcalfe DD. Mast cells in innate immunity. Immunol Rev. 2000;173: 131-140.  https://doi.org/10.1034/j.1600-065X.2000.917305.x
  27. Gilfillan AM, Tkaczyk C. Integrated signalling pathways for mast-cell activation. Nat. Rev. Immunol. 2006;6:218-230.  https://doi.org/10.1038/nri1782
  28. KAWASHIMA H, KIMURA Y, ITO.T. Cases of Allergic Rhinitis Successfully Treated with Tokishakuyakusan. Kampo Medicine. 2018;1:359-365. https://doi.org/10.3937/kampomed.69.359