Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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The Effect of Scalp Acupuncture and rTMS on Neuromotor Function in Photothrombotic Stroke Rat Model

  • Jong-Seong Park (Department of Physiology, Research Institute of Medical Sciences, Chonnam National University Medical School) ;
  • Eun-Jong Kim (Department of Physical & Rehabilitation Medicine, Research Institute of Medical Sciences, Chonnam National University Medical School) ;
  • Min-Keun Song (Department of Physical & Rehabilitation Medicine, Research Institute of Medical Sciences, Chonnam National University Medical School) ;
  • Jung-Kook Kim (Department of Physical & Rehabilitation Medicine, Research Institute of Medical Sciences, Chonnam National University Medical School) ;
  • Ganbold Selenge (Department of Physical & Rehabilitation Medicine, Research Institute of Medical Sciences, Chonnam National University Medical School) ;
  • Sam-Gyu Lee (Department of Physical & Rehabilitation Medicine, Research Institute of Medical Sciences, Chonnam National University Medical School)
  • Received : 2023.11.22
  • Accepted : 2023.12.01
  • Published : 2023.12.31
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Abstract

This study aimed to investigate effect of scalp acupuncture (SA) and repetitive transcranial magnetic stimulation (rTMS) intervention on neuromotor function in photothrombotic cerebral infarction (PCI) rat model. Sixty male SD rats were used. PCI was induced on M1 cortex of right frontal lobe. SA was performed at the Qianding (GV21), Xuanli (GB6) acupoints of ipsilesional M1. Low-frequency rTMS was delivered to contralesional M1. All rats were randomly divided into 4 groups: group A, normal (n, 15); group B, PCI without any stimulation intervention (n, 15); group C, PCI with SA (n, 15); group D, PCI with rTMS (n, 15). Rota-rod test and Ladder rung walking test (LWT) were done weekly for 8 weeks after PCI. SA or rTMS was started from post-PCI 4th day as protocol for 8 weeks. H/E stain and IHC were done. Western blot and qRT-PCR study were performed for MAP2 and BDNF from ipsilesional M1 peri-infarction tissue. Brain MRI study was conducted to quantify the volume of cerebral infarction. As a result, left forelimb and hindlimb function significantly improved more in group C and D than control group, with expressed more BDNF and MAP2. And brain MRI showed focal infarction of right M1 after PCI, and infarction volume progressively decreased in group C and D than group B from post-PCI 5th to 8th week. SA or rTMS was more effective than no intervention group on neuromotor function of PCI rat model. The functional recovery was associated with stimulation intervention-related neurogenesis.

Keywords

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (NRF-2017R1D1A1A02019434).

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