Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Two Previously Unrecorded Fungal Species Isolated from Muui Island in Korea

  • Soobin Shin (Department of Agricultural Biotechnology, Seoul National University) ;
  • Hosung Jeon (Department of Agricultural Biotechnology, Seoul National University) ;
  • Sieun Kim (Department of Agricultural Biotechnology, Seoul National University) ;
  • Hyun-Ju Noh (Division of Microbiology, Honam National Institute of Biological Resources) ;
  • Jong Won Jo (Division of Microbiology, Honam National Institute of Biological Resources) ;
  • Kyunghun Min (Department of Agricultural Biotechnology, Seoul National University) ;
  • Hokyoung Son (Department of Agricultural Biotechnology, Seoul National University)
  • Received : 2023.08.08
  • Accepted : 2023.11.29
  • Published : 2023.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Fungi are cosmopolitan and they occupy diverse niches as consumers, producers, and decomposers. They play critical roles in the environment by enabling nutrient cycling and generating a plethora of secondary metabolites. This study aimed to identify and characterize fungal strains isolated from diverse sources on Muui Island, Republic of Korea. In 2023, a total of 86 fungal strains were collected and examined. Investigation of the morphological features and phylogenetic analyses of multiple barcode loci identified one putative novel species and two species previously unrecorded in the Republic of Korea: Colletotrichum sp., Colletotrichum guizhouense, and Fusarium brachygibbosum. This study provides a comprehensive description of their molecular phylogenies and morphological characteristics. These findings will contribute to the existing knowledge about fungal species in the Republic of Korea and future research on the fungal diversity on Muui Island.

Keywords

Acknowledgement

This study was supported by the research grant, "Survey of Indigenous Species in Korean Islands" (HNIBR202101111) from the Honam National Institute of Biological Resources of the Ministry of Environment in Korea.

References

  1. National Institute of Biological Resources. National list of species of Korea, 2022. [cited 2023 July 25]. Available from: https://kbr.go.kr/. 
  2. Kim H-J, Son DC, Lee D-H, et al. Flora of vascular plants in Mueuido (Incheon), Korea. Korean J Environ Biol. 2016;34(4):246-256. doi: 10.11626/KJEB.2016.34.4.246. 
  3. Leslie JF, et al. The Fusarium laboratory manual. Ames, Iowa: Blackwell Publishing. 2006. p. 57-77. 
  4. White TJ, Bruns TD, Lee SB, et al. Amplification and direct sequencing of fungal ribosomal RNA Genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York: Academic Press. 1990. p. 315-322. 
  5. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 1999;91(3):553-556. doi: 10.2307/3761358. 
  6. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol. 1995;61(4):1323-1330. doi: 10.1128/aem.61.4.1323-1330.1995. 
  7. O'Donnell K, Cigelnik E. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol. 1997;7(1):103-116. doi: 10.1006/mpev.1996.0376. 
  8. Crouch JA, Clarke BB, Hillman BI. Unraveling evolutionary relationships among the divergent lineages of Colletotrichum causing anthracnose disease in turfgrass and corn. Phytopathology. 2006;96(1):46-60. doi: 10.1094/PHYTO-96-0046. 
  9. Kumar S, Stecher G, Li M, et al. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35(6):1547-1549. doi: 10.1093/molbev/msy096. 
  10. Edler D, Klein J, Antonelli A, et al. raxmlGUI 2.0: a graphical interface and toolkit for phylogenetic analyses using RAxML. Methods Ecol Evol. 2021;12(2): 373-377. doi: 10.1111/2041-210X.13512. 
  11. Schneider CA, Rasband WS, Eliceiri KW. NIH image to ImageJ: 25 years of image analysis. Nat Methods. 2012;9(7):671-675. doi: 10.1038/nmeth.2089. 
  12. Tao G, Liu Z-Y, Liu F, et al. Endophytic Colletotrichum species from bletilla ochracea (Orchidaceae), with descriptions of seven new speices. Fungal Divers. 2013;61(1):139-164. doi: 10.1007/s13225-013-0254-5. 
  13. Liu F, Ma ZY, Hou LW, et al. Updating species diversity of Colletotrichum, with a phylogenomic overview. Stud Mycol. 2022;101(1):1-56. doi: 10.3114/sim.2022.101.01. 
  14. Jayawardena R.S, Hyde KD, Wang S, et al., Fungal diversity notes 1512-1610: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Divers, 2022. 117(1): p. 1-272. doi: 10.1007/s13225-022-00513-0. 
  15. EFSA Panel on Plant Health (PLH). Pest categorisation of Fusarium brachygibbosum. Efsa J. 2021;19(11):e06887. 
  16. Boutigny A-L, Gautier A, Basler R, et al. Metabarcoding targeting the EF1 alpha region to assess Fusarium diversity on cereals. PLoS One. 2019;14(1):e0207988. doi: 10.1371/journal.pone.0207988. 
  17. Paul NC, Park S, Liu H, et al. Fungi associated with postharvest diseases of sweet potato storage roots and in vitro antagonistic assay of Trichoderma harzianum against the diseases. JoF. 2021;7(11):927. doi: 10.3390/jof7110927. 
  18. Kim D-H, Kim S-H, Kwon S-W, et al. The mycobiota of air inside and outside the meju fermentation room and the origin of meju fungi. Mycobiology. 2015;43 (3):258-265. doi: 10.5941/MYCO.2015.43.3.258. 
  19. Al-Mahmooli IH, Al-Bahri YS, Al-Sadi AM, et al. First report of euphorbia larica dieback caused by Fusarium brachygibbosum in Oman. Plant Dis. 2013;97(5):687-687. doi: 10.1094/PDIS-09-12-0828-PDN. 
  20. Jasarevic M, Catalani A, Morales-Rodriguez C, et al. First report of Fusarium brachygibbosum causing rots on potato tubers in Italy. J Plant Pathol. 2022; 104(4):1591-1591. doi: 10.1007/s42161-022-01221-z.