Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Screening and functional validation of lipid metabolism-related lncRNA-46546 based on the transcriptome analysis of early embryonic muscle tissue in chicken

  • Ruonan, Chen (College of Animal Science and Technology, Shihezi University) ;
  • Kai, Liao (College of Pharmacy, Shihezi University) ;
  • Herong, Liao (College of Animal Science and Technology, Shihezi University) ;
  • Li, Zhang (College of Animal Science and Technology, Shihezi University) ;
  • Haixuan, Zhao (College of Medical, Shihezi University) ;
  • Jie, Sun (College of Animal Science and Technology, Shihezi University)
  • Received : 2021.09.20
  • Accepted : 2022.01.07
  • Published : 2023.02.01
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Abstract

Objective: The study was conducted to screen differentially expressed long noncoding RNA (lncRNA) in chickens by high-throughput sequencing and explore its mechanism of action on intramuscular fat deposition. Methods: Herein, Rose crown and Cbb broiler chicken embryo breast and leg muscle lncRNA and mRNA expression profiles were constructed by RNA sequencing. A total of 96 and 42 differentially expressed lncRNAs were obtained in Rose crown vs Cobb broiler chicken breast and leg muscle, respectively. lncRNA-ENSGALT00000046546, with high interspecific variability and a potential regulatory role in lipid metabolism, and its predicted downstream target gene 1-acylglycerol-3-phosphate-O-acyltransferase 2 (AGPAT2), were selected for further study on the preadipocytes. Results: lncRNA-46546 overexpression in chicken preadipocyte 2 cells significantly increased (p<0.01) the expression levels of AGPAT2 and its downstream genes diacylglycerol acyltransferase 1 and diacylglycerol acyltransferase 2 and those of the fat metabolism-related genes peroxisome proliferator-activated receptor γ, CCAAT/enhancer binding protein α, fatty acid synthase, sterol regulatory element-binding transcription factor 1, and fatty acid binding protein 4. The lipid droplet concentration was higher in the overexpression group than in the control cells, and the triglyceride content in cells and medium was also significantly increased (p<0.01). Conclusion: This study preliminarily concludes that lncRNA-46546 may promote intramuscular fat deposition in chickens, laying a foundation for the study of lncRNAs in chicken early embryonic development and fat deposition.

Keywords

Acknowledgement

This research was funded by the National Natural Science Foundation of China (No. 31660654 & No.31860641).

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