DOI QR코드

DOI QR Code

과수화상병 매몰방재지 토양에서 분리된 8종의 국내 미기록 진균 보고

Report of Eight Unrecorded Fungi from the Fire Blight Burial Control Soil in Korea

  • 노형진 (단국대학교 생명과학부 미생물학전공) ;
  • 서희주 (단국대학교 생명과학부 미생물학전공) ;
  • 김성환 (단국대학교 생명과학부 미생물학전공)
  • Hyeongjin Noh (Department of Microbiology, Dankook University) ;
  • Huiju Seo (Department of Microbiology, Dankook University) ;
  • Seong Hwan Kim (Department of Microbiology, Dankook University)
  • 투고 : 2023.10.27
  • 심사 : 2023.12.26
  • 발행 : 2023.12.31

초록

식물병원 세균 Erwinia amylovora 의해 발생하는 과수화상병은 사과, 배 등 장미과에 속하는 식물에 심한 피해를 주고 있다. 병의 빠른 확산을 막기 위해 국내에서는 병이 발생한 사과나무와 배나무에 대해 매몰하는 병관리를 실시해 왔다. 방제방법의 안전성을 입증하기 위해 매몰한 이병식물과 이병식물 주변의 토양을 조사하였다. 매몰토양 미생물군 조사과정에서 Bisifusarium allantoides, Botryotrichum Domesticum, Microascus verrucosus, Paraphoma pye, Phaeosphaeria culmorum, Ramophialophora globispora, Sordaria tomentoalba, Striaticonidium brachysporum 등이 국내 미기록종으로 확인되었다. 이에 이들 8종 진균에 대한 형태학적, 분자유전학적 특성을 보고한다.

Fire blight, a plant disease caused by Erwinia amylovora, continues to cause considerable damage to fruit-bearing trees belonging to the Rosaceae family, such as apples and pears. In Korea, to hinder its rapid spread diseased apple and pear trees have been buried since its first occurrence. To determine the safety of this control method, the buried plant materials and adjacent soil have been investigated. In the process of investigating the microbiota of the buried soil, Bisifusarium allantoides, Botryotrichum domesticum, Microascus verrucosus, Paraphoma pye, Phaeosphaeria culmorum, Ramophialophora globispora, Sordaria tomentoalba, and Striaticonidium brachysporum were identified as unrecorded species in Korea. To report the above eight species as Korean unrecorded species, we report their morphological and molecular features in this study.

키워드

과제정보

This work was supported by grants from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE), the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ016242); RDA, and the Animal and Plant Quarantine Agency of the Republic of Korea.

참고문헌

  1. Zwet T, Keil HL. Fire blight: a bacterial disease of Rosaceous plants. Handbook 510. Washington, D.C.: United States Department of Agriculture; 1979. 
  2. Cho JW, Roh E, Lee YH, Kim SH. Fire blight management status in partial control applied orchards. Res Plant Dis 2023;29:316-20.  https://doi.org/10.5423/RPD.2023.29.3.316
  3. Gusberti M, Klemm U, Meier M, Maurhofer M, Hunger-Glaser I. Fire blight control: the struggle goes on. A comparison of different fire blight control methods in Switzerland with respect to biosafety, efficacy and durability. Int J Environ Res Public Health 2015;12:11422-47.  https://doi.org/10.3390/ijerph120911422
  4. Kim YE, Kim JY, Noh HJ, Lee DH, Kim SS, Kim SH. Investigating survival of Erwinia amylovora from fire blight-diseased apple and pear trees buried in soil as control measure. Korean J Environ Agric 2019;38:269-72.  https://doi.org/10.5338/KJEA.2019.38.4.36
  5. Yun YH, Hyun MW, Suh DY, Kim YM, Kim SH. Identification and characterization of Eurotium rubrum isolated from Meju in Korea. Mycobiology 2009;37:251-7.  https://doi.org/10.4489/MYCO.2009.37.4.251
  6. White TJ, Bruns T, Lee SJ, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: a Guide to Methods and Applications 1990;18:315- 22.  https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  7. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018;35:1547. 
  8. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673-80.  https://doi.org/10.1093/nar/22.22.4673
  9. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Bio Evol 1987;4:406-25. 
  10. Savary O, Coton M, Frisvad JC, Nodet P, Ropars J, Coton E, Jany JL. Unexpected Nectriaceae species diversity in cheese, description of Bisifusarium allantoides sp. nov., Bisifusarium penicilloides sp. nov., Longinectria gen. nov. lagenoides sp. nov. and Longinectria verticilliforme sp. nov. Mycosphere J 2021;12:1077-100.  https://doi.org/10.5943/mycosphere/12/1/13
  11. Schultes NP, Strzalkowski N, Li DW. Botryotrichum domesticum sp. nov., a new hyphomycete from an indoor environment. Botany 2019;97:311-19.  https://doi.org/10.1139/cjb-2018-0196
  12. Marchal E. Champignons coprophiles de Belgique, IVIV. Bull Soc R Bot Belge 1885;24:57-77. 
  13. Sandoval-Denis M, Gene J, Sutton DA, Cano-Lira JF, De Hoog GS, Decock CA, Wiederhold NP, Guarro J. Redefining Microascus, Scopulariopsis and allied genera. Pers: Mol Phylogeny Evol Fungi 2016;36:1-36.  https://doi.org/10.3767/003158516X688027
  14. Marques AR, Kwon-Chung KJ, Holland SM, Turner ML, Gallin JI. Suppurative cutaneous granulomata caused by Microascus cinereus in a patient with chronic granulomatous disease. Clin Infect Dis 1995;20:110-4.  https://doi.org/10.1093/clinids/20.1.110
  15. Huang L, Chen W, Guo L, Zhao L, Cao B, Liu Y, Lu B, Li B, Chen J, Wang C. Scopulariopsis microascus isolation in lung transplant recipients: a report of three cases and a review of the literature. Mycoses 2019;62:883-92.  https://doi.org/10.1111/myc.12952
  16. Moslemi A, Ades PK, Crous PW, Groom T, Scott JB, Nicolas ME, Taylor PW. Paraphoma chlamydocopiosa sp. nov. and Paraphoma pye sp. nov., two new species associated with leaf and crown infection of pyrethrum. Plant Pathol 2018;67:124-35.  https://doi.org/10.1111/ppa.12719
  17. Irinyi L, Gade AK, Ingle AP, Kovics GJ, Rai MK, Sandor E. Morphology and molecular biology of Phoma. Curr Res Environ Appl Mycol 2009;2009:171-203. 
  18. Leuchtmann A. uber Phaeosphaeria Miyake und andere bitunicate Ascomyceten mit mehrfach querseptierten Ascosporen [dissertation]. Zurich: ETH Zurich; 1984. 
  19. Zhang ZF, Liu F, Zhou X, Liu XZ, Liu SJ, Cai L. Culturable mycobiota from Karst caves in China, with descriptions of 20 new species. Pers: Mol Phylogeny Evol Fungi 2017;39:1-31.  https://doi.org/10.3767/persoonia.2017.39.01
  20. El-Demerdash A. Chemical diversity and biological activities of Phaeosphaeria fungi genus: a systematic review. J Fungi 2018;4:130. 
  21. Calduch M, Gene J, Stchigel AM, Cano JF, Guarro J. Ramophialophora, a new anamorphic genus of Sordariales. Stud Mycol 2004;50:83-8. 
  22. Kirk PM, Cannon PF, Minter DW, Stalpers JA. Dictionary of the fungi 10th ed Wallingford: CABI; 2008. p. 643-44. 
  23. Cailleux R. Champignons stercoraux de Republique Centrafricaine. Recherches sur la mycoflore coprophile centrafricaine. Les genres Sordaria, Gelasinospora, Bombardia. Bull Trimest Soc Mycol Fr 1971;87:461-626. 
  24. Lombard L, Houbraken J, Decock C, Samson RA, Meijer M, Reblova M, Groenewald JZ, Crous PW. Generic hyper-diversity in Stachybotriaceae. Persoonia 2016;36:156-246.  https://doi.org/10.3767/003158516X691582
  25. Nicot J, Olivry C. Contribution a l'etude du genre Myrothecium Tode. 1. Les especes a spores striees. Rev Gen Bot 1961;68:672-85. 
  26. Azizi R, Ghosta Y, Ahmadpour A. New fungal canker pathogens of apple trees in Iran. J Crop Prot 2020;9:669-81.