Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Improving Biomass Productivity of Freshwater microalga, Parachlorella sp. by Controlling Gas Supply Rate and Light Intensity in a Bubble Column Photobioreactor

가스공급속도 및 광도조절을 이용한 담수미세조류 Parachlorella sp.의 바이오매스 생산성 향상

  • Z-Hun Kim (Huevergreenpharm Inc.) ;
  • Kyung Jun Yim (Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Seong-Joo Hong (Department of Biological Engineering & Industry-Academia Interactive R&E Center for Bioprocess Innovation, Inha University) ;
  • Huisoo Jang (Industrial Science and Technology Research Institute, Inha University) ;
  • Hyun-Jin Jang (Department of Food Science and Nutrition, Jeju National University) ;
  • Suk Min Yun (Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Seung Hwan Lee (Department of Biotechnology and Bioengineering, Chonnam National University) ;
  • Choul-Gyun Lee (Department of Biological Engineering & Industry-Academia Interactive R&E Center for Bioprocess Innovation, Inha University) ;
  • Chang Soo Lee (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
  • 김지훈 ((주)휴에버그린팜) ;
  • 임경준 (국립낙동강생물자원관 미생물연구실) ;
  • 홍성주 (인하대학교 생명공학과 & 산학융합 인터랙티브 바이오공정 혁신 교육연구단) ;
  • 장희수 (인하대학교 산업과학기술연구소) ;
  • 장현진 (제주대학교 식품영양학과) ;
  • 윤석민 (국립낙동강생물자원관 미생물연구실) ;
  • 이승환 (전남대학교 생물공학과) ;
  • 이철균 (인하대학교 생명공학과 & 산학융합 인터랙티브 바이오공정 혁신 교육연구단) ;
  • 이창수 (국립낙동강생물자원관 미생물연구실)
  • Received : 2023.05.10
  • Accepted : 2023.08.08
  • Published : 2023.12.31
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Abstract

The objective of the present study was to improve the biomass productivity of newly isolated freshwater green microalga Parachlorella sp. This was accomplished by culture conditions optimization, including CO2 concentration, superficial gas velocity, and light intensity, in 0.5 L bubble column photobioreactors. The supplied CO2 concentration and gas velocity varied from 0.032% (air) to 10% and 0.02 m/s - 0.11 m/s, respectively, to evaluate their effects on growth kinetics. Next, to maximize the production rate of Parachlorella sp., a lumostatic operation based on a specific light uptake rate (qe) was applied. From these results, the optimal CO2 concentration in the supplied gas and the gas velocity were determined to be 5% and 0.064 m/s, respectively. For the lumostatic operation at 10.2 µmol/g/s, biomass productivity and photon yield showed significant increases of 83% and 66%, respectively, relative to cultures under constant light intensity. These results indicate that the biomass productivity of Parachlorella sp. can be improved by optimizing gas properties and light control as cell concentrations vary over time.

Keywords

Acknowledgement

이 연구는 환경부의 재원으로 국립낙동강생물자원관에서 지원(NNIBR202303113)과 과학기술정보통신부의 재원으로 한국연구재단의 지원을 받아 수행된 연구입니다(NRF-2021R1A2C2005148).

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